Searchable abstracts of presentations at key conferences in endocrinology
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Society for Endocrinology BES 2015

Edinburgh, UK
02 Nov 2015 - 04 Nov 2015

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2-4 November 2015, Edinburgh, UK Further information

Poster Presentations


ea0038p347 | Reproduction | SFEBES2015

Analysis of the human foetal gonadal proteome at 13–14 weeks of gestation

Matthews Rebecca , Filis Panagiotis , O'Shaughnessy Peter , Fowler Paul

Introduction: The human masculinisation programming window (8–14 weeks of gestation) sees testis-derived androgen drive the foetus towards a male phenotype. However, there are few systematic studies of human foetal gonad development.Aim: To conduct a pilot analysis of the foetal gonadal proteome at the end of the masculinisation programming window (13–14 weeks of gestation).Methods: Twenty-eight electively terminated foet...

ea0038p348 | Reproduction | SFEBES2015

Development of a single-injection non-surgical sterilant via modification of measles virus pseudotyped particles

Roesl Cornelia , Jeffery Nathan , Smith Sarah , Milne Laura , Brown Pamela , Smith Lee

Feral cat numbers in the UK are thought to number around one million and the Dogs Trust estimates it dealt with just over 110 000 stray and abandoned dogs in 2013. This scenario is common across the world and in the developing world in particular the control of feral populations is an ongoing challenge as well as a huge welfare issue. The only options currently available are expensive surgical neutering, or euthanasia of otherwise healthy animals. There is an urgent need to de...

ea0038p349 | Reproduction | SFEBES2015

Decoding gonadotrophin receptor signalling via spatial regulation of the LH receptor

Sposini Silvia , Jean-Alphonse Frederic , Hanyaloglu Aylin

The LH receptor (LHR) is essential for mediating multiple functions in reproduction and pregnancy. LHR belongs to the superfamily of G-protein-coupled receptors (GPCRs) that impacts nearly every aspect of human physiology and pathophysiology. Membrane trafficking is a critical mechanism for cells to decode complex signalling networks into specific downstream responses, including the signalling pathways activated by GPCRs. A detailed molecular description of GPCR trafficking is...

ea0038p350 | Reproduction | SFEBES2015

IGF1 action in trophoblast involves endocytic and post-endocytic pathways

Karolczak-Bayatti Magda , Horn James , Case Patrick , Harris Lynda , Aplin John , Westwood Melissa

IGF1 plays a central role in placental growth. IGF delivered from maternal circulation binds to the IGF1 receptor (IGF1R) in syncytium resulting in the activation of Akt; inhibition of this pathway affects cytotrophoblast turnover. However, the route(s) by which IGF1 enters placenta and how its signal is delivered into the two trophoblast compartments is unknown. We have utilised quantum dots (QDs) to deliver/track IGF1 binding and internalisation in first trimester placenta (...

ea0038p351 | Reproduction | SFEBES2015

Intracrine androgens enhance decidualisation and modulate expression of human endometrial receptivity genes

Gibson Douglas , Simitsidellis Ioannis , Critchley Hilary , Saunders Philippa

During the establishment of pregnancy, the endometrium undergoes dynamic remodelling in order to establish a ‘receptive’ microenvironment. Decidualisation, a key part of this process, is characterised by differentiation of endometrial stromal fibroblasts which secrete factors that regulate implantation and placental development. Recent studies in our laboratory have revealed that decidualisation results in altered expression of enzymes that regulate biosynthesis and ...

ea0038p352 | Reproduction | SFEBES2015

I.v. and s.c. infusions of kisspeptin-54 stimulate gonadotrophin release similarly in healthy women

Narayanaswamy Shakunthala , Jayasena Channa , Ng Noel , Ratnasabapathy Risheka , Papadopoulou Deborah , Prague Julia , Abbara Ali , Comninos Alexander , Bloom Stephen , Dhillo Waljit

Background: Kisspeptin stimulates hypothalamic GnRH secretion resulting in gonadotrophin release and has the potential as a future therapeutic for infertility. Previous studies have observed that kisspeptin increases LH and to a lesser degree FSH when administered to healthy women, which may limit its therapeutic potential. However, studies in women with hypothalamic amenorrhoea show that i.v. infusions of kisspeptin-54 stimulated both LH and FSH equally. Chronic s.c. infusion...

ea0038p353 | Reproduction | SFEBES2015

Assessment of reproductive parameters and oxidative stress following treatment with alcohol and/or cannabinol in male Wistar rats

Akintayo Christopher , Ayodele Moyo , Karga Shimile

The male gonads have been exposed to various degrees of toxicants over the years and these substances affect fertility success rate. The present study investigates the effects of alcohol and/or cannabinol administration on reproductive parameters and biochemical profile in male Wistar rats. Twenty-five Wistar male rats were divided into five groups of five rats each. Group 1 served as the control, group 2 was administered methanol, 5 mg/kg BW (vehicle for cannabinol), group 3 ...

ea0038p354 | Reproduction | SFEBES2015

Increased expression of circulating miRNA-93 in women with polycystic ovary syndrome may represent a novel, non-invasive biomarker for diagnosis

Sathyapalan Thozhukat , David Rhiannon , Gooderham Nigel J , Atkin Stephen L

Introduction: MicroRNAs (miRNA) are a novel class of small noncoding single-stranded RNA molecules 18–24 nucleotides long that regulate gene expression at the post-transcriptional level. There is increasing evidence of their importance in polycystic ovary syndrome (PCOS), with their differential expression depending on obesity. There is recent evidence that miRNA-93 and mi-RNA-223 may have a role to play in the modulation of insulin resistance, through GLUT4 modulation in...

ea0038p355 | Reproduction | SFEBES2015

Vitamin D regulates extravillous trophoblast migration by inhibiting sphingosine-1-phosphate (S1P) signalling via S1P receptor 2

Johnstone Edward , Al-Saghir Khiria , Tan Cherlyn , Adlam Daman , Westwood Melissa

Failure of trophoblast invasion and remodelling of maternal blood vessels leads to the pregnancy complication pre-eclampsia (PE). Metabolomic profiling of placentas from such pregnancies has identified deranged sphingolipid metabolism as one of the pathways altered in PE. In other systems, the bioactive sphingolipid, sphingosine-1-phosphate (S1P) controls cell migration therefore this study aimed to determine its effect on extravillous trophoblast (EVT) function.<p class="...

ea0038p356 | Reproduction | SFEBES2015

Visceral fat drives 5α-reductase activity independent of BMI in women with polycystic ovarian syndrome

Kempegowda Punith , O'Reilly Michael W , Crabtree Nicola J , Taylor Angela E , Hughes Beverly A , Tomlinson Jeremy W , Arlt Wiebke

Context: Androgen excess, obesity and hyperinsulinaemia are the cardinal features of polycystic ovarian syndrome (PCOS). While several studies have addressed the relationship between androgen excess and hyperinsulinaemia, the link between androgen excess and fat distribution remains largely undefined. Recent work has highlighted the importance of adipose tissue as an organ of androgen activation. Here, we evaluated the relationship between visceral fat and androgen excess in w...

ea0038p357 | Reproduction | SFEBES2015

Impact of male cholestasis on the sperm epigenome and consequences for the health of the offspring

Pataia Vanessa , Papacleovoulou Georgia , Poston Lucilla , Williamson Catherine

Introduction: Accumulating evidence has shown that not only maternal health during pregnancy, but also the paternal metabolic status at the time of conception have an impact on the subsequent health of the offspring. Cholestatic liver diseases are metabolic conditions characterised by increased circulating serum bile acid and lipid levels. A previous study has shown that long-term cholestasis results in destruction of the blood–testis barrier and germ cell apoptosis. We h...

ea0038p358 | Reproduction | SFEBES2015

Isoform-specific knockdown of PKC isoforms reveals PKCβ2 is required for epidermal growth factor stimulated expression of the prostaglandin synthase COX2 in human myometrium

Hudson Claire , Bernal Andres Lopez

Intrauterine prostaglandin (PG) synthesis and release are key components of human labour at term and pre-term, resulting in synchronised membrane rupture, cervical dilation and myometrial contractility. A key mediator of uterine PG production is the highly inducible enzyme cyclooxygenase 2 (COX2 or PTGS2). Epidermal growth factor (EGF) is released by the foetus during pregnancy and amniotic fluid levels rise rapidly during late gestation, moreover EGF stimulates expression of ...

ea0038p359 | Reproduction | SFEBES2015

Information transfer in GnRH signalling: ERK-mediated feedback loops control hormone sensing

Garner Kathryn , Perrett Rebecca , Voliotis Margaritis , Pham Thanh , Tsaneva-Atanasova Krasimira , McArdle Craig

Single cell measurements of signalling proteins typically reveal high cell–cell variability raising questions about how reliably individual cells sense their environment in order to make decisions. Information theoretic approaches can be used to explore such sensing, treating cell signalling pathways as ‘noisy’ communication channels. Mutual information (MI) can be calculated between system inputs and outputs as a statistical measure of the reliability of sensin...

ea0038p360 | Reproduction | SFEBES2015

Whole exome sequencing in congenital hypogonadotropic hypogonadism

Izatt Louise , Carroll Paul , Lillis Suzanne , Brodd Lina , Stone Kristina , Ryan Kevin , Ahn JooWook , Simpson Michael , Yau Michael

Congenital hypogonadotropic hypogonadism (CHH (MIM161110)) due to GnRH deficiency is a rare genetic disorder (affects ~1/30 000) characterised by abnormal pubertal development and infertility. Over 60% cases have anosmia (Kallmann syndrome) and some exhibit additional phenotypes. CHH is a genetically heterogeneous developmental disease. Most cases present sporadically, although familial forms (AD, AR, and X-linked) with incomplete penetrance and variable expressivity occur. Re...

ea0038p361 | Reproduction | SFEBES2015

Implantation and pregnancy outcome of rats fed with low and high salt diet

Bolanle Iranloye , Gabriel Oludare

Minerals and micronutrients deficiency have been reported to impair reproductive function. We investigated the influence of low salt diet and high salt diet on implantation and pregnancy in Sprague-Dawley rats. One hundred and forty-four rats were acclimatized and divided into three groups consisting of control/normal salt diet (0.3% salt), low salt diet (0.14% salt) and high salt diet (8% salt). All the rats were fed with respective diets for 6 weeks. The oestrous cycle was t...

ea0038p362 | Reproduction | SFEBES2015

Vitamin D metabolic profiling across pregnancy

Susarla Radhika , Jenkinson Carl , Tamblyn Jennifer , Keevil Brian , Chan Shiao-Yng , Kilby Mark , Hewison Martin

Vitamin D-deficiency during pregnancy has been associated with increased complications of pregnancy including a high risk of pre-eclampsia (PET). Current analysis of vitamin D ‘status’ is based exclusively on analysis of maternal serum 25-hydroxyvitamin D3 (25(OH)D3), the circulating precursor form of vitamin D. We hypothesised that comprehensive profiling of vitamin D metabolites may provide a more accurate determination of vitamin D function i...

ea0038p363 | Reproduction | SFEBES2015

Vitamin D and foetomaternal immunity: effects on uterine natural killer cells

Tamblyn Jennifer , Jeffery Louisa , Susarla Radhika , Moss Paul , Hewison Martin , Kilby Mark

Vitamin D deficiency is prevalent in pregnant women. Active vitamin D (1,25(OH)2D3) exerts important non-classical immune-regulatory effects, and the maternal placenta (decidua) is a potential target for this. CD56+ -uterine natural killer (uNK) cells are the most prominent cell type in the decidua during early pregnancy. Given their critical role in foetal implantation and placentation, we hypothesised that uNK cells are a pivotal immunomodula...

ea0038p364 | Reproduction | SFEBES2015

Use of an animal model to identify the origin and validity of the testicular dysgenesis syndrome hypothesis in humans

van den Driesche Sander , Kilcoyne Karen , Wagner Ida , Boyle Ashley , McKinnell Chris , Macpherson Sheila , Mitchell Rod , Sharpe Richard

From human epidemiological and related studies, there is strong (indirect) evidence that common male reproductive disorders that manifest at birth (cryptorchidism, hypospadias) or in adulthood (low sperm count, low testosterone, primary hypogonadism) may have a common origin in foetal life due to impaired androgen (testosterone) production or action; the so-called testicular dysgenesis syndrome (TDS) hypothesis. Whilst the foetal origin of cryptorchidism and hypospadias is sel...

ea0038p365 | Reproduction | SFEBES2015

Investigation into the effects of glucocorticoids in a mouse model of induced menstruation

Murray Alison A , Armstrong Gregory M , Murgai Reena , Gray-Renfrew Alexandra E , Vere Rebecca E A , Chapman Karen E , Critchley Hilary O D

Introduction: Heavy menstrual bleeding (HMB) affects over 1 million women in the UK. At menses, progesterone (P4) withdrawal drives inflammation, tissue break-down and repair of the endometrium. Glucocorticoids are mediators of inflammation and angiogenesis. Our previous studies have demonstrated that differential endometrial expression of the glucocorticoid-metabolising enzymes, 11β-HSD-1 and -2, may play a role in HMB. We hypothesise that aberrant lo...

ea0038p366 | Reproduction | SFEBES2015

Structured education programme to improve cardiovascular risk in women with polycystic ovary syndrome: SUCCESS-RCT improved physical activity and illness perception

Mani Hamidreza , Khunti Kamlesh , Levy Miles , Bodicoat Danielle , Gray Laura , Barnett Janette , Daly Heather , Howlett Trevor , Davies Melanie

Background: Structured education programmes (SEP) have proved effective in changing illness perception and increasing physical activity (PA) in those with or at risk of diabetes. The SUCCESS–RCT was designed to test such a programme in women with polycystic ovary syndrome (PCOS).Methods: This was a single centre, randomised controlled trial in overweight and obese women with PCOS aimed at increasing their walking activity by 2000 steps/day after 12 ...

ea0038p367 | Reproduction | SFEBES2015

Characterisation of a GnRH-type signalling system in an echinoderm

Tian Shi , Zandawala Meet , Egertova Michaela , Beets Isabel , Slade Susan E , Scrivens James H , Elphick Maurice R

Gonadotropin-releasing hormone (GnRH) regulates secretion of the reproductive hormones luteinising hormone (LH) and follicle-stimulating hormone (FSH) in mammals. Homologs of GnRH have been identified in protostomian and deuterostomian invertebrates, and functional studies suggest an ancient role in regulation of reproductive physiology. The aim of this study was to characterise for the first time a GnRH-type signalling system in an echinoderm, using the starfish Asterias ...

ea0038p368 | Reproduction | SFEBES2015

Low-dose gonadotrophin therapy for induction of ovulation: comparison of results in women with PCOS or hypothalamic amenorrhoea

Lovelock Suzie , Hardy Kate , White Davinia , Franks Stephen

We have reviewed the outcome of a low-dose step-up regimen for induction of ovulation in clomiphene-unresponsive women with PCOS and in women with hypothalamic hypogonadism (HH), treated at a single centre. Data from 366 women with PCOS and 80 with HH (1165 records) were entered into a FileMakerPro database, refined and constrained to allow stratification and analysis of relevant data. 85% of cycles were ovulatory in both PCOS and HH. PCOS patients were more likely to have cyc...

ea0038p369 | Reproduction | SFEBES2015

Characterisation of bile acid pathways in steroidogenic tissues

Jarvis Sheba , Gadaleta Raffaela M , Want Elizabeth J , Gray Nicola , Abu-Hayyeh Shadi , Winston Lord Robert , Williamson Catherine , Bevan Charlotte L

Background: Bile acids (BAs) are end-products of cholesterol catabolism, which act as signalling molecules to regulate glucose, lipid and energy metabolism. BAs activate several receptors including the ligand sensitive transcription factor Farnesoid X receptor (FXR) and the membrane G-protein coupled receptor, TGR5. Besides the organs physiologically in contact with BAs, like the gut and liver, BA-receptors are also expressed in cholesterol-rich steroidogenic tissues, such as ...

ea0038p370 | Reproduction | SFEBES2015

Food, drink and medical plants and their molecules can affect female hormones and reproductive functions

Sirotkin Alexander V , Kadasi Attila , Stochmalova Aneta , Alexa Richard , Balazi Andrej , Harrath Abdel Halim

The aim of our studies was to examine the effect of some food, drink and medical plants and their molecules on female ovarian endocrine and other functions in vitro and in vivo. We analysed the effects of plant (green tea, rooibos, ginkgo, flaxseed, chia, yukka) extracts and plant substances (green tea polyphenols, green tea epigallocatechin-3-gallate, curcumin, resveratrol, diadzein and diosgenin) on the release of hormones and markers of proliferation, apop...

ea0038p371 | Reproduction | SFEBES2015

Regulation of Cyp17a1 expression in mouse ovarian theca cells in vivo and in vitro

Kakuta Hanako , Sato Tomomi

Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in reproductive-aged women. Its most typical form is the association of hyperandrogenism with chronic anovulation. Theca cells (TC) of large antral and ovulatory follicles are the main source of ovarian androgens and express P450c17α, encoded by Cyp17a1, which converts progesterone to 17α-hydroxyprogesterone and androstenedione. It is known that Cyp17a1 expression in TC f...

ea0038p372 | Reproduction | SFEBES2015

Maternal insulin-like growth factor-1 and transforming growth factor beta-1 levels during pregnancy in rats fed with low and high salt

Oludare Gabriel , Iranloye Bolanle

Low birth weight has been reported in the offspring of rats fed with low salt diet. This study estimated the levels of maternal growth factors of pregnant rats fed with low salt and high salt diet. Seventy-two female Sprague–Dawley rats were acclimatized and divided into three groups consisting of control/normal salt diet (0.3% salt), low salt diet (0.14% salt) and high salt diet (8% salt). Rats were fed for six weeks and the cycles of the rats were observed in the 6th we...

ea0038p373 | Reproduction | SFEBES2015

Identification of novel transcription factors that may regulate transcription of the equine chorionic gonadotrophin beta subunit

Read Jordan , Cabrera-Sharp Victoria , Mirczuk Samantha , Fowkes Robert , de Mestre Amanda

Equine Chorionic Gonadotrophin (eCG) is a heterodimeric glycoprotein produced by terminally differentiated, bi/multi-nucleate trophoblast cells in the placenta of horses and humans. It is responsible for the maintenance of early pregnancy via rescue of the corpus luteum and subsequently promotion of progesterone production. The beta subunit of eCG is expressed at levels ten-fold lower than that of the alpha subunit and confers the glycoproteins specificity to the placenta. Ver...

ea0038p374 | Reproduction | SFEBES2015

A rare form genetic abnormality in Turner syndrome

Costache-Outas Mariana-Cristina , Procopiuc Camelia , Popa Oana , Vintila Madalina-Aura , Costache Cosmina-Raluca , Stambouli Danai

Turner Syndrome (TS) is defined as the combination of characteristic physical features in phenotypic females and complete or partial absence of the second sex chromosome. Short stature is a constant clinical finding in patients with TS. We report the case of a 18 year old female with TS and normal stature. Primary amenorrhea was the reason for the first clinical presentation. Laboratory evaluation showed hypergonadotropic hypogonadism, low oestrogens and testosterone. A pelvic...

ea0038p375 | Reproduction | SFEBES2015

Long-term monitoring of hypogonadal men receiving intramuscular testosterone replacement: a retrospective audit

Rys Andrzej , Candan Jonathan , Cox Sue , Smith Jamie

Background: Intramuscular long acting testosterone injections are widely used, convenient form of androgen replacement in patients with hypogonadism.Aims: To evaluate the long term effects of parenteral long acting testosterone replacement on patients commenced on treatment in years 2006–2014 and adherence to monitoring undertaken by primary care after discharge from specialist service.Method: We reviewed the results of 64 pat...

ea0038p376 | Reproduction | SFEBES2015

Prevalence and correlates of premature ejaculation among Nigerians in a tertiary health centre: a preliminary cross-sectional study

Olamoyegun Michael , Akinlade Akinyele , Ajani David , Aransiola Clement , Enang Ofem , Gbadegesin Babajide , Olopade Rotimi , Ojo Olujide

Background: Premature ejaculation (PE) is believed to be one of the most common male sexual disorders across all age groups. However its prevalence and risk factors has been rarely investigated among Nigerians. Hence, the study determined the prevalence and risk factors for erectile dysfunction (ED) especially PE among this populace.Subjects and methods: This cross-sectional survey was conducted on seventy-three (73) males aged 18-75years who were sexual...

ea0038p377 | Reproduction | SFEBES2015

Effects of oral, injectable, and implant hormonal contraception on serum levels of iron, ferritin, copper, ceruloplasmin and vitamin E

Samuel Titilola , Morakinyo Olufemi , Balogun Adepeju , Olamijulo Jo , Magbagbeola Olubunmi

Recently there has been reports that the administration of hormonal contraceptives alter the metabolic processes and micronutrients levels. Meanwhile some of these micronutrients are cofactors and or coenzymes of enzymes and are involved in important metabolic pathways. We compared the influence of three contraceptive methods (oral contraceptive, injectables and implants) on serum levels of Iron, ferritin, copper, ceruloplasmin and Vitamin E, using standard methods. Blood samp...

ea0038p378 | Reproduction | SFEBES2015

Importance of social support and implications of gender reassignment in congenital adrenal hyperplasia due to 3-β hydroxysteroid deficiency

Kandaswamy Leelavathy , Raghavan Rajeev , Cherukuri Vijay Nandini , Buch Harit

Introduction: 3-β-hydroxysteroid dehydrogenase (3BHSD) deficiency is a rare genetic disorder of steroid biosynthesis that results in decreased production of all three groups of adrenal steroids, which include mineralocorticoids, glucocorticoids, and sex steroids.Case presentation: An infant was born with ambiguous genitalia in 1975 to a conservative Muslim family and was registered as male. The child was hospitalised with addisonian crisis on the el...

ea0038p379 | Reproduction | SFEBES2015

Testicular regression syndrome and severe psychiatric disorder – a rare association preventing the optimal management of the endocrine condition

Capatina Cristina , Ghinea Adela , Deciu Diana , Poiana Catalina

Introduction: Testicular regression syndrome (TRS) or vanishing testis syndrome is a rare condition defined as the absence of testicular tissue in a genetic and phenotypic male. Rudimentary accessory structures can be present; in that case, the removal of all remnants is recommended.Case report: Male patient, 39 years old, had initially been evaluated at the age of 8 for persistent bilateral cryptorchidism. The medical documents are lacking except for a ...

ea0038p380 | Reproduction | SFEBES2015

Self medication in transpeople is not associated with deterioration in cardiovascular risk factors but is associated with reduced vitamin D levels and antidepressant use

Seal Leighton , Khaleque Fahmin , Barrett James

Objective: This is a prospective audit looking at cardiovascular parameters in transpeople comparing those who have self medicated verses those who have not.Methods: Patients attending a workshop for new patients were questioned about cardiovascular risk factors including diabetes hypertension and smoking status. Measurements were made of lipid profile, glucose, blood pressure, waist, height and weight measurement. Those that were self medicating (SM) we...

ea0038p381 | Reproduction | SFEBES2015

A pragmatic review of patient satisfaction and testosterone replacement therapy

Smith Adam , Dickinson Sarah , Arber Michael

Introduction: In addition to safety and efficacy, patient satisfaction with treatment is potentially a key factor in maintaining therapy adherence. Testosterone replacement therapy (TRT) is provided using a variety of products with different features and routes of administration. These differing product characteristics may impact on patient satisfaction and adherence with a concomitant impact on well-being and health-related quality of life. The aim of this study was to explor...

ea0038p382 | Reproduction | SFEBES2015

Spontaneous resolution of hypothalamic amenorrhea post diagnostic GnRH test

Dimitriadis G K , Weickert M O , Barber T M , Randeva H S

Introduction/background: Hypothalamic Amenorrhea (HA), or stress-induced anovulation, is one of the most common causes of secondary amenorrhea (1,2) and accounts for the reproductive dysfunction seen in under nutrition, excessive exercise, sever emotional stress and chronic disease. From a teleological standpoint, in the face of nutritional or physical stress, it is adaptive for an organism to allocate energy resources for its own survival rather than the costly process of rep...