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Endocrine Abstracts (2015) 38 P362 | DOI: 10.1530/endoabs.38.P362

1University of Birmingham, Birmingham, UK; 2University Hospital of South Manchester NHS Foundation, Manchester, UK.


Vitamin D-deficiency during pregnancy has been associated with increased complications of pregnancy including a high risk of pre-eclampsia (PET). Current analysis of vitamin D ‘status’ is based exclusively on analysis of maternal serum 25-hydroxyvitamin D3 (25(OH)D3), the circulating precursor form of vitamin D. We hypothesised that comprehensive profiling of vitamin D metabolites may provide a more accurate determination of vitamin D function in pregnancy. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to separate multiple vitamin D metabolites from serum samples obtained from: normal pregnant women (NP) at 1st trimester (n=22) and 3rd trimester (n=21); women with PET (n=21); non-pregnant female controls (n=20). Data (see table, Mean±S.E.M.) demonstrated that active (1α,25(OH)2D3, P<0.001), catabolic (24,25(OH)2D3, P<0.05) and inactive (Epi-25(OH)D3, P<0.01) are increased in serum from 3rd trimester and PET pregnancies relative to NP women. Epi-25(OH)D3 was elevated in all women across trimesters (P<0.01). By contrast, conventionally measured 25(OH)D3 showed no significant change in any of the groups. Ratio of 24,25(OH)2D3/1α,25(OH)2D3 was 100.8±2 in normal 3rd trimester, and 258.9±39 in PET (P<0.05). Ratio of Epi-25(OH)D3/1α,25(OH)2D3 was 77.4±7 in normal 3rd trimester, and 172.3±29 in PET (P<0.01). These data, suggests a shift towards catabolic pathway of vitamin D metabolism in PET. The albumin (decreases) and DBP (increases) levels inversely correlated with gestation (Table 1).

Table 1
25(OH)D3 (nM)Epi-25(OH)D3 (nM)lα,25(OH)2D3 (pM)24,25(OH)D3 (nM)DBP (μm)Albumin (μm)
Non-pregnant35.18±3.85.04±0.442.04±4.14.66±0.951.69±0.21557.04±28.86
1st trimester34.7±4.347.98±0.3752.36±5.172.84±0.652.32±0.25554.43±25.85
3rd Trimester44.61±5.047.64±0.41108.34±6.6710.6±2.122.48±0.31330.06±21.07
PET38.45±5.949.49±0.7677.84±9.2715.96±2.562.38±0.31409.26±20.15

Measurement of multiple vitamin D metabolites and metabolites ratios may improve the interpretation of vitamin D status in pregnancy, possibly as an additional marker of adverse pregnancy events such as PET.

Volume 38

Society for Endocrinology BES 2015

Edinburgh, UK
02 Nov 2015 - 04 Nov 2015

Society for Endocrinology 

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