Searchable abstracts of presentations at key conferences in endocrinology
Previous issue | Volume 49 | ECE2017 | Next issue

19th European Congress of Endocrinology

Lisbon, Portugal
20 May 2017 - 23 May 2017

Card image cap
20-23 May 2017, Lisbon, Portugal Further information

ea0049ep1095 | Female Reproduction | ECE2017

Administration of first line anti-tuberculosis drugs induces ovarian and uterine oxidative stress and disruption of endocrine balance in rats

Adaramoye Oluwatosin , Adebayo Olayinka , Adesanoye Omolola , Abolaji Amos , Kehinde Aderemi

The first line anti-tuberculosis (anti-TB) drugs; isoniazid (INH), rifampicin (RIF), ethambutol (EMB) and pyrazinamide (PZA) were effective in the treatment of pulmonary tuberculosis. However, the toxicity of these drugs has been of great concern in clinical settings. This study was designed to evaluate the toxic effects of anti-TB drugs on reproductive system in female rats. Thirty-five female Wistar rats were assigned into five groups of seven animals each. The control recei...

ea0049ep1096 | Female Reproduction | ECE2017

Development of hepatic steatosis and inflammation by chronic insulin and hCG exposure in female rats: possible implications in PCOS patients with NAFLD

Shao Ruijin , Zhang Yuehui , Hu Min , Billig Hakan

Accumulating clinical data suggest that women with polycystic ovary syndrome (PCOS) are at high risk for nonalcoholic fatty liver disease (NAFLD). However, it is not clear whether hyperinsulinemia and hyperandrogenism act concomitantly or independently to induce hepatic steatosis and inflammation, and the molecular mechanisms behind the interactions between insulin resistance and hyperandrogenism in the female liver remain largely unexplored. To achieve hyperinsulinemia, insul...

ea0049ep1097 | Female Reproduction | ECE2017

Frequency of nodular goiter and autoimmune thyroid disease and association of these disorders with insulin resistance in polycystic ovary syndrome

Karakose Mellia , Hepsen Sema , Cakal Erman , Arslan Muyesser , Tutal Esra , Akin Safak , Unsal Ilknur , Ozbek Mustafa

Background and aim: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorder in women with reproductive age. Nodular goiter and autoimmune thyroid disease (AITD) are frequently seen endocrinological abnormalities. The aim of this study was to identify the prevalence of AITD and nodular goiter in PCOS patients and investigate whether PCOS related hormones and metabolic factors affect these thyroid disorders.Methods: Ninety-seven with ...

ea0049ep1098 | Female Reproduction | ECE2017

Association of basal and post-synacthen stimulated 17-hydroxyprogesterone levels with insulin resistance in polycystic ovary syndrome

Kandaraki Eleni , Papadakis Georgios , Tsirona Sofia , Asimakopoulou Athina , Chiotinis Nikolaos , Chronopoulou Genovefa , Kassi Georgia , Diamanti-Kandarakis Evanthia

Objective: 17-alpha-hydroxyprogesterone caproate therapy during pregnancy has been associated with insulin resistance. The aim of this study was to highlight any possible association of 17OHP with insulin resistance in women with PCOS.Design: Forty-five women with PCOS underwent 75 g OGTT test and short Synachten test with sampling of stimulated 17OHP. Women were divided into two subgroups according to basal 17OHP cut-off level of 0.8 ng/ml.<p class=...

ea0049ep1099 | Female Reproduction | ECE2017

Serum sclerostin and dickkopf-1 levels in polycystic ovary syndrome patients

Bilem Ogun , Bilem Yildiz , Eroglu Mustafa , Turkon Hakan , Akdeniz Yasemin Sefika , Asik Mehmet

Introduction: Polycystic ovary syndrome (PCOS) is the most common endocrine pathology in premenopausal women and it is a complex syndrome affecting various systems. Numerous studies have been made about how the PCOS effects on bone metabolism, but currently there is no clear information. Wnt pathway plays important role in the regulation of bone metabolism. The sclerostin(Scl) and Dickkopf-1 (DKK1) inhibit this pathway and they has recently become a therapeutic target of osteo...

ea0049ep1100 | Female Reproduction | ECE2017

Phthalates induce ovarian failure through disturbance in folliculogenesis and steroidogenesis

Tran Dinh Nam , Lee Jae Hwan , Ahn Changhwan , An Jin Yong , Park Seon Young , Jeung Eui-Bae

Introduction: Phthalates are chemicals used to improve the plasticity of industrial polymers and used in commercial products such as toys, paints, packaging materials, medicals devices and personal care items. Phthalates, endocrine disrupting chemicals, have been documented to cause adverse effects to the human health such as breast cancer in female, reduced uroogenital distance and changed the expression of steroidogenesis and folliculogenessis. In this study, we investigated...

ea0049ep1101 | Female Reproduction | ECE2017

Efficacy comparison of oral rosuvastatin versus oral progesterone and bevacizumab on regression of surgically endometriotic implants in rats

Kebapcilar Ayse Gul , Ilhan Tolga Tuyan , Dursunoglu Duygu , Kurt Kubra , Ipekci Suleyman Hilmi , Baldane Suleyman , Ucar Mustafa Gazi , Kirac Cem Onur , Kebapcilar Levent , Celik Cetin

Aim: Thirty female Wistar albino rats with surgically endometriotic implants were randomly randomized into three treatment groups: oral rosuvastatin (20 mg kg per day; oral rosuvastatin group 1; n=10), oral progesterone (Dienogest group 2; n=10), and intraperitoneal bevacizumab (2.5 mg/kg of single intaperitoneal injection of bevacizumab; group 3; n=10) respectively, for 10 day and post-treatment variables were compared.Results...

ea0049ep1102 | Female Reproduction | ECE2017

The relation between sex hormones and sexual function in women with Turner syndrome

Kriksciuniene Ruta , Zilaitiene Birute , Sematonyte Justina , Verkauskiene Rasa

Objectives: To evaluate sexual function in women with Turner syndrome (TS) in the relation with sex hormones.Methods: The study was undertaken in Lithuanian University of Health Sciences. 65 women with genetically confirmed TS (18–45 years) were enrolled and compared with 65 age-matched healthy controls. Sexual function was evaluated using The Female Sexual Function Index (FSFI). Risk for sexual dysfunction was defined as FSFI score ≤ 26.55. B...

ea0049ep1103 | Female Reproduction | ECE2017

BMP8 sustains expansion and survival of cumulus cells in vitro

Wu Fang-Ju , Luo Ching-Wei

Cumulus cells are a group of specialized granulosa cells that surrounds oocytes and are critical for female fertility. During ovulation, cumulus cells need to be expanded systematically in order to support the maturation, ovulation and fertilization of oocytes, whereas their apoptosis will result in the decline of fertilization rate and pregnancy outcome. However, the molecules as well as their signalings involved in the control of cumulus cell-oocyte complex (COC) expansion a...

ea0049ep1104 | Female Reproduction | ECE2017

Serum concentrations of betatrophin and its association with indirect indices of insulin resistance and beta cell function in women with polycystic ovary syndrome

Adamska Agnieszka , Lebkowska Agnieszka , Jacewicz Malgorzata , Krentowska Anna , Hryniewicka Justyna , Wolczynski Slawomir , Gorska Maria , Kowalska Irina

The accumulated data underline the role of betatrophin in glucose homeostasis. Polycystic ovary syndrome (PCOS) is characterized by insulin resistance and high risk of developing prediabetes and diabetes. The aim of our study was to investigate the relationship of serum betatrophin concentrations with indirect indices of insulin resistance and insulin secretion in women with PCOS, comparing to the control group. The study group comprised 43 women with PCOS and 16 controls matc...

ea0049ep1105 | Female Reproduction | ECE2017

Short term intervention with liraglutide and metformin increased fertility potential in a subset of obese PCOS proceeding IVF

Šalamon Vesna , Jensterle Mojca , Janež Andrej , Bokal Eda Vrtačnik

Objective: Obese PCOS have poor IVF outcomes. The aim of this study was to evaluate the impact of weight reduction with metformin alone or in adjunct with liraglutide on oocyte maturity and embryo quality in infertile obese PCOS population.Design/participants/main outcome measure: A 12-week prospective randomized study was conducted with 40 infertile PCOS (30.77±3.742 aged years, BMI 36.69±3.51 kg/m2, mean ± SD) who had been pre...

ea0049ep1106 | Female Reproduction | ECE2017

The functional state of hypophysis – gonad axis in patients with polycystic ovary syndrome (PCOS)

Urmanova Yulduz , Khodgaeva Feruza , Alieva Dinara , Sodikov Salim , Rikhsieva Nazira , Abduvakhabova Maryam

The aim: To study the functional state of axis of hypophysis-gonads for women with PCOS.Material and methods of investigation: Under our supervision in the department of neuroendocrinology of The Center of Endocrinology of PHM of Republic of Uzbekistan ambulatory in a period from September 2015 for July, 2016 120 patients of fertile age were inspected with polycystic ovary syndrome (PCOS). Middle age of patients – 25.5±4.3 years. The remoteness...

ea0049ep1107 | Female Reproduction | ECE2017

Efficacy of FSH alone, FSH + LH, hMG or FSH + hCG on ART outcomes in the ‘personalized’ medicine era: a meta-analysis

Santi Daniele , Casarini Livio , Alviggi Carlo , Simoni Manuela

Background: Luteinizing hormone (LH) and human chorionic gonadotropin (hCG) act on the same receptor, activating different signal transduction pathways. The role of LH or hCG addition to follicle stimulating hormone (FSH) as well as menopausal gonadotropins (hMG) in controlled ovarian stimulation (COS) is debated.Aim: To compare FSH+LH, or FSH+hCG or hMG vs FSH alone on COS outcomes.Design: A meta-analysis according to PRISMA state...

ea0049ep1108 | Female Reproduction | ECE2017

May thiol/disulfide homeostasis predict adult PCOS?

Sevimli Hakan , Koca Cemile , Senyurt Mahmut , Erel Ozcan , Laloglu Esra , Carlioglu Ayse

Purpose: The purpose of this study was to evaluate the relation polycystic ovarian syndome (PCOS) and the thiol/disulfide balance, used as a marker of oxidative stress, by measuring that exchange using a novel technique.Material/methods: Fourty nine subjects diagnosed with PCOS and 44 healthy were included in the study. Thiol/disulfide homeostasis concentrations were measured by a newly developed method. After native thiol, total thiol and disulfide leve...

ea0049ep1109 | Female Reproduction | ECE2017

Effects of cigarette smoke extracts on proliferation, migration, and hCG-β protein expression of JEG-3 human placental cancer cells

Kim Cho-Won , Lee Hae-Miru , Choi Kyung-Chul

Maternal smoking during pregnancy is known to be related to adverse pregnancy results associated with trophoblast proliferation and cell cycle progression. Moreover, many previous studies have shown that cigarette smoke is correlated with human chorionic gonadotropin beta (hCG-β) subunit produced from syncytiotrophoblasts during pregnancy. Thus, we further investigated whether cigarette smoke extract (CSE) affects the cell proliferation, migration and endocrine hormone ac...

ea0049ep1110 | Female Reproduction | ECE2017

Hyperandrogenism and overweight/obesity, independently and interactively, increase the risk of metabolic syndrome and type 2 diabetes in women – a Prospective, Population-based Cohort Study

Ollila Meri-Maija , Kaikkonen Kari , Keinanen-Kiukaanniemi Sirkka , Puukka Katri , Ruokonen Aimo , Jarvelin Marjo-Riitta , Tapanainen Juha , Franks Steve , Piltonen Terhi , Morin-Papunen Laure

The significance of hyperandrogenism (HA) as a metabolic risk factor in women is controversial. HA can be defined as either clinical (hirsutism) or biochemical (elevated androgen levels). We aimed to investigate whether HA at age 31 associates with metabolic syndrome (MetS), type 2 diabetes mellitus (DM2) or carotid intima media thickness (CIMT) by age 46. In a prospective, general population-based follow-up birth cohort (n=5889 women) blood samples were collected at ...

ea0049ep1111 | Female Reproduction | ECE2017

Natural history of the Swyer Syndrome

Doroszewska Katarzyna , Milewicz Tomasz , Kialka Marta , Mrozinska Sandra

We present a case of a eighteen-year-old woman admitted to the Gynecological and Endocrinological Department because of primary amenorrhea. Physical examination showed excessive pubic hair and clitoral hypertrophy. Laboratory tests showed the increased level of foliculotropina (FSH) 90 mIU/ml (normal range 3.5–12.5 mIU/ml) and the estradiol level below 10 pmol/l (normal range 46–607 pmol/l). An ultrasound-imaging of the abdomen and pelvis showed the uterus of normal ...

ea0049ep1112 | Female Reproduction | ECE2017

Biological activity mediated by sfingosine-1-phospate receptors in human primary granulosa cells and immortalized granulosa cell line in vitro

Casarini Livio , Fornari Giulia , Simoni Manuela , Poti Francesco

Introduction: Sphingosine-1-phosphate (S1P) is a sphingolipid mediating proliferative signals in human ovarian follicles, acting through five receptors (S1PR1-5). We characterized the effects of S1PR1 and S1PR3 in vitro, as those mostly expressed in human primary granulosa cells (hGLC) and immortalized, tumor-like granulosa cell line (hGL5).Methods: The S1P-mediated signaling, as well as the activity of S1PR1- and S1PR3-specific agonists (SEW281...

ea0049ep1113 | Female Reproduction | ECE2017

Polycystic ovary syndrome and euthyroid hashimoto’s thyroiditis: possible influence of thyroid autoantibodies

Utlu Mustafa , Timur Ozge , Carlioglu Ayse

Thyroid disorders and polycystic ovary syndrome (PCOS) are two of the most common endocrine disorders in general population. Autoimmune thyroid diseases are common autoimmune disorders that affect about 5% to 20% of women in childbearing age. In this study, we evaluate the comorbidity rate of euthyroid Hashimoto’s Thyroiditis (HT) in PCOS patients and possible influence of thyroid autoantibodies on clinical and laboratory findings of PCOS. This study includes 285 PCOS pat...

ea0049ep1114 | Female Reproduction | ECE2017

Novel insulin resistance index using C-peptide is reliable predictor of insulin resistance in women with PCOS

Bozic-Antic Ivana , Ilic Dusan , Bjekic-Macut Jelica , Vojnovic-Milutinovic Danijela , Ognjanovic Sanja , Bogavac Tamara , Popovic Bojana , Isailovic Tatjana , Elezovic Valentina , Stanojlovic Olivera , Macut Djuro

Introduction: Recently, it was suggested that insulin resistance index using C-peptide (CIR) could be a better marker of insulin resistance than homeostasis model assessment (HOMA-IR) in patients with type 2 diabetes mellitus. The aim of this study was to investigate performance of CIR index in PCOS subjects.Methods: We evaluated 187 PCOS women (PCOS: 25.38±6.32 kg/m2; 26.01±5.16 years) diagnosed using ESHRE/ASRM criteria and 42 heal...

ea0049ep1115 | Female Reproduction | ECE2017

Omentin-1, a potential link between chronic low grade inflammation, metabolic and reproductive features of polycystic ovary syndrome

Shorakae Soulmaz , Abell Sally , de Courten Barbora , Lambert Gavin , Hiam Danielle , Stepto Nigel , Jona Eveline , Moran Lisa , Teede Helena

Background: Polycystic ovary syndrome (PCOS) is associated with features linked to metabolic syndrome including visceral adiposity, dyslipidemia and impaired glucose homeostasis. Adipose tissue produces adipocytokines which contribute to regulation of insulin sensitivity and reproduction. Omentin-1 is an adipokine with anti-inflammatory and insulin sensitizing roles produced by adipocytes. The exact role of mentin-1 in PCOS remains unclear as studies have reported reduced or u...

ea0049ep1116 | Female Reproduction | ECE2017

Hirsutism in reproductive aged Korean women

Sung Yeon Ah , Baik Sei Hyun , Kim Dong Sun

Hirsutism, excessive terminal hair growth in a male-like pattern as a marker of hyperandrogenism, affects 5–10% of reproductive-age women worldwide. The frequency of hirsutism seems to decrease in Asians and the lower cut-off modified hirsutism score is suggested. We performed the study to estimate the frequency, bio-metabolic characteristics, correlates and determinants of hirsutism in reproductive aged Korean women. A total of 2682 female volunteers of reproductive age ...

ea0049ep1117 | Female Reproduction | ECE2017

Pharmacokinetics and bioavailability of non-polar phytocomponents of Aloe vera gel and their role as an endocrine modulator in letrozole induced PCOS rat model

Dey Arpi , Vasoya Shweta , Nampoothiri Laxmipriya

Background and aim: Polycystic ovary syndrome (PCOS), the most common endocrine disorder in women of reproductive age with an estimated prevalence of 5–10%, is one of the most common causes of female infertility. The therapeutic options for PCOS are limited. Thereby, traditional knowledge of Ayurveda has been exploited to identify an herbal therapeutic target for PCOS. Pharmacokinetic (PK) studies on bioactive constituents of herbal drugs provide valuable information on b...

ea0049ep1118 | Female Reproduction | ECE2017

Influence of peroxisome proliferator-activated receptor (PPAR)-γ exon 2 (Pro12Ala) and exon 6 (His447His) and Gly972Arg insulin receptor substrate (IRS)-1 polymorphisms on insulin resistance (IR) and beta cell function in Southern Mediterranean women with polycystic ovary syndrome (PCOS)

Russo Giuseppina T , Pappalardo Maria Angela , Giandalia Annalisa , Romeo Elisabetta L , Alibrandi Angela , Bari Flavia Di , Vita Roberto , Cucinotta Domenico , Benvenga Salvatore

The Pro12Ala and His447His polymorphisms of PPAR-γ, and Gly972Arg polymorphism of IRS-1 have been implicated in insulin resistance (IR) and adiposity. In this study, we investigated the possible influence of these polymorphisms on metabolic features of 53 PCOS women compared with 26 healthy women (controls). All women underwent a clinical, anthropometric and biochemical evaluation, including a 75-g oral glucose tolerance test; insulin secretion and sensitivity indices wer...

ea0049ep1119 | Female Reproduction | ECE2017

Hormonal disturbances and adipokines levels in girls with oligomenorrhea

Skrzypulec-Plinta Violetta , Drosdzol-Cop Agnieszka , Sajdak Dominika

Aim: The aim of this study was to evaluate hormonal disturbances and adipokines levels in girls with oligomenorrhea.Materials and methods: The study comprised a group of 46 girls, aged 16–18 years, who were diagnosed with oligomenorrhea, and a control group with 37 healthy girls, aged 16–18 years, with no diagnosed menstrual disorders. In the first stage of the study, all girls had their medical history assessed. Subsequently, anthropometric me...

ea0049ep1120 | Female Reproduction | ECE2017

Global methylation pattern and endocrine-metabolic profile during early infancy and puberty in sons born to women with polycystic ovary syndrome (PCOS)

Sir-Petermann Teresa , Crisosto Nicolas , Echiburu Barbara , Maliqueo Manuel , Perez-Bravo Francisco , Sandoval Daniel , Recabarren Sergio

Prenatal and postnatal environment can regulate gene expression through multiple epigenetic mechanisms, being DNA methylation among the most relevant. Metabolic and reproductive function may be modified by this mechanism. Sons born to women with PCOS show altered markers of metabolic and reproductive function during childhood and adulthood. However, these functions have not been studied during puberty. The aim of the present study was to assess the global methylation pattern a...

ea0049ep1121 | Female Reproduction | ECE2017

Natural history of autoimmune primary ovarian insufficiency progresses through several stages from normal ovary function to clinically overt ovary dysfunction

Bellastella Giuseppe , Casciano Ofelia , Barrasso Mariluce , Maiorino Maria Ida , Giugliano Dario , Esposito Katherine , Scappaticcio Lorenzo , Bellis Annamaria De

Context: Women with autoimmune Addison’s disease with normally ovulatory cycles but positive for steroid cells antibodies (StCA) have been considered at risk of premature ovarian insufficiency (POI).Design: Thirty-three women younger than 40 years, with subclinical-clinical Addison’s disease but with normally ovulatory menses, were followed-up for 10 years to evaluate the long-term time-related variations of StCA, ovarian function and follicula...

ea0049ep1122 | Female Reproduction | ECE2017

Relationship between anabolic hormones and benign lesions in different organs in premenopousal women

Tekin Zeynep Nilufer , Aycicek Bercem , Demirbaş Tuna

Objective: We aim to detect co-occurence of uterine myomas, thyroid nodules and breast lesions in premenoposual women with menstruel disorders and investigate association between these benign tumors with anabolic hormon levels.Design: Records of 8008 premenopausal women were analyzed who had been admitted to the hospital for routine breast image investigation which was performed by the same radiologist in between 2010 and 2015.Stud...

ea0049ep1123 | Female Reproduction | ECE2017

Heterogeneous hCG and hMG commercial preparations result in biased intracellular signaling but induce similar progesterone response in vitro

Riccetti Laura , Klett Daniele , Ayoub Mohammed Akli , Boulo Thomas , Mariot Julie , Pignatti Elisa , Tagliavini Simonetta , Varani Manuela , Tranti Tommaso , Nicoli Alessia , Sala Giovanni Battista La , Combarnous Yves , Reiter Eric , Simoni Manuela , Casarini Livio

Human chorionic gonadotropin (hCG) is the pregnancy hormone marketed as a drug for assisted reproduction technologies to support follicle-stimulating hormone action. Several hCG formulations are commercially available, differing in source, purification methods and biochemical composition. In this study, we investigated the molecular features and the intracellular signaling mediated by five urinary and recombinant hCGs. The drug comparisons were performed by quantifying the hor...

ea0049ep1124 | Female Reproduction | ECE2017

Etiopathogenetical aspects of endocrine infertility

Xilola Gulyamova , Kamilla Ibragimova

Urgency: According to WHO the frequency of infertile unions is high amount 25–30% in developed countries. There are following reasons of infertility by women: 1) tubal infertility; 2) endocrine infertility-it takes the 2nd place among the reasons of infertility; 3) anomalies of the vagina and uterus; 4) immunological reasons, that are caused by sensibilisation of female organism, also there are patients, the reason of infertility of that is not ‘explainabl...

ea0049ep1125 | Female Reproduction | ECE2017

Prenatal androgen exposure alters gene expression and promoter methylation of two ovarian follicle maturation factors

Jahromi Marziyeh Salehi , Tehrani Fahimeh Ramezani , Hill Jennifer W

Background: Follicles imaturation, as the hallmark of polycystic ovarian syndrome caused by prenatal androgen exposure, is suspected to be effected through expression changes of related genes. The aim of this study was to evaluate gene expression and promoter methylation of GATA6 and Follistatin (FST), two factors involved in follicle maturation, in adult female rats prenatally exposed to androgen excess by comparing them with non-treated rats.Material a...

ea0049ep1126 | Female Reproduction | ECE2017

Graduated response to pulsatile GnRH therapy in hypothalamic amenorrhea

Germain Natacha , Fauconnier Anais , Klein Jean-Philippe , Wargny Amelie , Khalfallah Yadh , Papastathi-Boureau Chrysoula , Estour Bruno , Galusca Bogdan

Introduction: Pulsatile GnRH therapy is currently used to restore LH pulse and induce physiological ovulation, with effectiveness demonstrated in all types of hypothalamic amenorrhea (HA). Anorexia nervosa (AN) is characterized by self-starvation-induced undernutrition leading to functional hypothalamic amenorrhea (HA). Weight recovery does not always restore menses despite no apparent clinical and biological undernutrition residual signs. Only few specific studies on persiste...

ea0049ep1127 | Female Reproduction | ECE2017

Relationship between steroid hormones and metabolic profile in women with polycystic ovary syndrome

Lazurova Zora , Figurova Jana , Ujhazi Simona , Mašlankova Jana , Toporcerova Silvia , Lazurova Ivica

Background: There is evidence that polycystic ovary syndrome (PCOS) is commonly associated with higher cardiometabolic risk. Aim of the study was to compare various sex streoids in PCOS women with overweight or obesity (BMI >27) and those with lower BMI. Second aim of the study was to determine the relationship of various sexual setroids to cardiovascular risk in PCOS women.Subjects and methods: Study included 64 Caucasian women with PCOS. F...

ea0049ep1128 | Female Reproduction | ECE2017

Secondary amenorrhea after bone marrow transplantation and adjuvant chemotherapy misdiagnosed as disorder of sex development: a case report

Elleuch Mouna , Ammar Mouna , Kacem Faten Hadj , Ghorbel Dorra , Rekik Nabila , Charfi Nadia , Kalthoum Mahdi , Mnif Mouna , Abid Mohamed

Introduction: Disorders of sex development (DSD) is a congenital condition in which the development of chromosomal, gonadal or genital sex is atypical. A female appearance patient with secondary amenorrhea and 46 XY karyotype seems to be solid evidences to diagnose Y-chromosome-related DSD diseases, while it is not necessarily the accurate diagnosis. We here report a case of a 16- year-old girl with secondary amenorrhea and 46 XY karyotype after bone marrow transplantation (BM...

ea0049ep1129 | Female Reproduction | ECE2017

NCAH prevalance with novel CYP21A2 and CYP11B1 mutations in hirsut turkish women

Polat Seher , Karaburgu Sulbiye , Unluhizarci Kursad , Dundar Munis , Ozkul Yusuf , Karaca Zuleyha , Kelestimur Fahrettin

Introduction: Hirsutism affects 5–8% of the whole female population. In most patients, hirsutism is associated with hyperandrogenemia and the most common cause of androgen excess is polycystic ovary syndrome; however, the clinical presentation of non-classical congenital adrenal hyperplasia(NCAH) in females is often indistinguishable from other hyperandrogenic disorders because of common clinical signs with hirsutism and poly cystic ovary syndrome(PCOS).<p class="abst...

ea0049ep1130 | Female Reproduction | ECE2017

Effects of photoperiod on estrous cycle in the Mongolian gerbil (Meriones unguiculatus)

Ortega Nayeli , Arteaga Marcela , Duran Pilar

Gerbil (Meriones unguiculatus) a non traditional laboratory model with reproductive state influenced by photoperiod, was used to analyze those photoperiodic mechanisms involving reproductive regulation. The 14:10 photoperiod has been widely used for reproductive research since 1950 (Everett). As is well known gerbils locomotor activity cycle has been reported to be crepuscular and reproductive cycle presents two annual peaks. As for the estrous cycle (EC), it has been...

ea0049ep1131 | Female Reproduction | ECE2017

The regulation of LHCGR-dependent signaling is linked to circadian gene expression

Gilioli Lisa , Marino Marco , Simoni Manuela , Casarini Livio

Introduction: Reproduction exhibit a regular cyclicity and is regulated by complex interactions between circadian signals, gonadotropins and sex steroid hormones. Peripheral circadian rhythm is generated upon cyclic transcription of clock genes expressed in several cells, e.g. granulosa cells in the ovary. Here we investigated whether cyclic intracellular events occurring in response to gonadotropins are linked to expression of clock genes.Methods: Prima...

ea0049ep1132 | Female Reproduction | ECE2017

High dose metformin effect on weightloss, androgen levels and thyroid function in obese hypothyroid patients with PCOS

Ranetti Aurelian-Emil , Cucu Anca-Pati

Objective: to evaluate the effects of metformin use on weight loss, improvement of ovarian and thyroid function in obese women with polycystic ovarian syndrome.Material and Methods: 30 obese (BMI 30–34.99 kg/m2) hypothyroid (TSH 6–10 mIU/ml) women of age 25–35 years old were included. The patients received appropriate levothyroxine treatment according to TSH levels and body weight. A number of 15 patients added metformin gradual...

ea0049ep1133 | Female Reproduction | ECE2017

Influence of thyroid stimulating hormone (TSH) level in in-vitro fertilization (IVF) success

Faria Carolina , Gomes Ana , Nunes Joaquim , Sousa Sandra , Ana Aguiar , Catia Rodrigues , Joaquim Sampaio , Carlos Calhaz-Jorge

Introduction: Prevalence of hypothyroidism is 2–5% in women in the reprodutive age group. Hypothyroidism can affect fertility due to anovulatory cycles, luteal phase defects, hyperprolactinemia and sex hormones imbalance. Some data also report that TSH levels are inversely proportional to the fertilization rate at ART (assisted reproductive technologies).Objective: To evaluate if a relationship exists between pregnancy rates in women undergoing ...

ea0049ep1134 | Female Reproduction | ECE2017

The efficiency of metformin in polycystic ovarian syndrome is not necessarily attributable to the improvement of insulin resistance

Szanto Zsuzsanna , Micheu Adelina

Insulin resistance has a pivotal role in the pathogenesis of polycystic ovarian syndrome (PCOS), thus insulin sensitizer therapy is beneficial. Purpose. Our purpose was to study the effectiveness of metformin in PCOS women, including those without insulin resistance. Matherials and methods. This study was conducted on 24 pacients (12 obese or overweight and 12 lean women), aged 21–31 years, diagnosed and treated with PCOS at the Clinical Department of Endocrinology Targu-...

ea0049ep1135 | Female Reproduction | ECE2017

Serum Vitamin D status in women with the polycystic ovary syndrome

Kuliczkowska-Plaksej Justyna , Milewicz Andrzej , Pasquali Renato , Lenarcik-Kabzda Agnieszka , Laczmanski Lukasz , Lwow Felicja , Jedrzejuk Diana , Bolanowski Marek

Objective: To measure the levels of 25-hydroxyvitamin D (25(OH)D) and vitamin D binding protein (VDBP) and assess their relationships with metabolic features in patients with the polycystic ovary syndrome (PCOS).Methods: 267 women, aged 20–35 years (24.7±4.9): 167 with PCOS, 100 healthy women as a control group were studied. Study groups were divided according to body mass index (BMI). Biochemical and hormonal parameters, 25(OH)D, VDBP were mea...

ea0049ep1136 | Female Reproduction | ECE2017

Abstract withdrawn....

ea0049ep1137 | Female Reproduction | ECE2017

Oxidative status imbalance in patients with polycystic ovary syndrome

Nicolae Alina Crenguta , Dragoi Cristina Manuela , Arsene Andreea Letitia , Burlacu Nicoleta Petruta , Filisan Crina , Albu Alice , Barbu Carmen Gabriela

Polycystic ovary syndrome (PCOS) is one of the most frequently encountered endocrine pathologies in women of reproductive age, being regarded as a chronic systemic disease instead of the simple local one. It is frequently associated with insulin resistance, hyperandrogenemia and chronic inflammation. PCOS reunites multiple and diversified pathological combinations like: ovulatory functional disorders directly impacting on the reproductive system, carbohydrate and lipid metabol...

ea0049ep1138 | Female Reproduction | ECE2017

Utility of oral glucose tolerance test (OGTT) in patients with polycystic ovary syndrome (PCOS)

Petre Oana Alexandra , Gheorghe Ivona , Nicolaescu Irina , Albu Dragos , Albu Alice

Background: PCOS patients associate an increased risk of glucose metabolism abnormalities. Although Hb A1c was suggested as a good diagnostic test for prediabetes/diabetes, in PCOS patients its diagnostic value could be inferior to OGTT as previously suggested. Aim of the study was to evaluate which are the clinical and paraclinical parameters associated with impaired glucose tolerance (IGT) in order to identify the profile of the patients that will benefit from OGTT.<p cl...

ea0049ep1139 | Female Reproduction | ECE2017

Diagnostic performance of non-invasive markers for the presence of nonalcoholic fatty liver disease in premenopausal women with and without polycystic ovary syndrome

Vassilatou Evangeline , Lafoyianni Sophia , Vassiliadi Dimitra-Argyro , Ioannidis Dimitrios , Paschou Stavroula , Mizamtzidi Maria , Vryonidou Andromachi

Introduction: Several markers based on anthropometric and biochemical parameters like fatty liver index (FLI), lipid accumulation product (LAP) index and visceral adiposity index (VAI), have been proposed for the detection of nonalcoholic fatty liver disease (NAFLD).Objective: To evaluate the diagnostic performance of FLI, LAP and VAI as markers of NAFLD in premenopausal women with and without polycystic ovary syndrome (PCOS), assessed for hepatic steato...

ea0049ep1140 | Female Reproduction | ECE2017

Complementary effect of Vitamin D supplementation and lifestyle modification on anthropometric and metabolic parameters in young polycystic ovary syndrome women with Vitamin D deficiency: a 3-month prospective interventional study

Jayanthy Ramesh , Belgundi Prasanna

Background: PCOS women usually have metabolic disturbances particularly insulin resistance. Previous studies suggest that vitamin D deficiency may contribute to the development of insulin resistance. Our aim is to study the effect of vitamin D supplementation on clinical and biochemical parameters in vitamin D deficient PCOS women.Methods: At baseline, 50 PCOS women were enrolled (37 vitamin D deficiency and 13 vitamin D sufficiency). Thirty-seven PCOS w...

ea0049ep1141 | Female Reproduction | ECE2017

Epigenetics of female hypogonadotropic hypogonadism: analysis of mRNA expression of engaged in IHH genes

Loktionova Anna , Ilovayskaya Irena , Eneva Natela , Khusniyarova Karina , Nefedova Lidia , Kim Alexander , Dreval Alexander

Hypogonadotropic hypogonadism is a syndrome which may be manifested clinically by low sex steroids because of low (or inappropriately normal) gonadotropins. To date, more than 30 genes were reported as possible reason for this condition, but genetic basis is still unknown for ~60% of HH cases. Due to this fact we decided to analyze mRNA expression of several HH-related genes which can be found in leukocytes of peripheral blood: GNRHR and GNRH1 (which are necessary for adequate...

ea0049ep1142 | Female Reproduction | ECE2017

How far is possible to make a diagnosis of infertility

Dias Daniela , Simoes-Pereira Joana , Donato Sara , Pereira Conceicao

Introduction: Chemotherapy may be an important cause of primary infertility, especially in girls submitted to this therapy at very young ages.Clinical case: A 20-year-old female was diagnosed with acute lymphoblastic leukaemia at the age of 1. She was submitted to chemotherapy: intrathecal methotrexate and cyclophosphamide and i.v. doxorubicin, vincristine, mercaptopurine, asparaginase and bone marrow transplantation at the age of 2. She was ref...

ea0049ep1143 | Female Reproduction | ECE2017

Physiological changes of adipokines and selected steroids during the menstrual cycle

Šramkova Monika , Duškova Michaela , Vitků Jana , Včelak Josef , Matucha Petr , Bradnova Olga , Cordeiro Jose , Starka Luboslav

Context: The cyclical effects of hormones during the menstrual cycle (MC) are responsible for driving ovulation. The information about roles of adipokines within the scope of MC are not definite. Leptin plays a role in sexual function and regulating the onset of puberty. Thin girls often fail to ovulate or release an egg from an ovary during menstruation cycles. Leptin also acts on specific receptors in the hypothalamus to inhibit appetite. Levels of leptin are increased in wo...

ea0049ep1144 | Female Reproduction | ECE2017

Serum activities of dipeptidyl peptidase-4 and adenosine deaminase and parameters of oxidative stress in polycystic ovary syndrome: association with obesity

Kahraman Seda , Altinova Alev Eroglu , Ulkar Serenay Elgun , Yalcin Mehmet Muhittin , Yilmaz Banu Aktas , Ozkan Cigdem , Akturk Mujde , Toruner Fusun Balos

Dipeptidyl peptidase 4 (DPP-4) is thought to play a role in the pathophysiology of metabolic and inflammatory diseases. Increased ADA activity has been suggested to induce insulin resistance and inflammation. We investigated DPP-4 and ADA activities, serum nitric oxide (NO) level and nitric oxide synthase (NOS) activity which are the oxidative stress parameters in patients with PCOS. Fifty two women with PCOS and 41 healthy women were included in this study. Serum ADA activity...

ea0049ep1145 | Female Reproduction | ECE2017

Oocyte donation in women with Turner’s syndrome: successful outcome can be achieved with a specialist multidisciplinary approach

Calanchini Matilde , Baker Kathy , Fabbri Andrea , Grossman Ashley , Orchard Elizabeth , Child Tim , Turner Helen

Introduction: Although oocyte donation (OD) is increasingly utilised in women with Turner’s syndrome (TS) few data are available. Reported clinical pregnancy rates following OD range from 17 to 40%. Complications of 2% death from aortic dissection and severe hypertension are reported.Aim: To analyse the OD-pregnancy success rate and materno-fetal outcomes in women followed in a TS-dedicated centre.Methods: A retrospective stud...

ea0049ep1146 | Female Reproduction | ECE2017

The features of young women reproductive health with type 1 diabetes

Kondratiuk Kateryna , Kobyliak Nazarii , Nikitina Iryna , Kondratiuk Valentyna , Bodnar Petro

Background: Leading position among endocrine pathology of adolescence and young women is type 1 diabetes (T1D), which in recent years had been proved to have a strong association with reproductive dysfunction among such patients. The purpose was to investigate the features of reproductive health in 120 young women (18–22 years old) with T1D.Methods: Retrospectively we studied the features of puberty and prospectively examined level of blood glucose ...

ea0049ep1147 | Female Reproduction | ECE2017

The determinants of thyroid autoimmunity in patients with polycystic ovary syndrome

Albu Alice , Petre Oana , Nicolaescu Irina , Gheorghe Ivona , Albu Dragos

It was suggested that polycystic ovary syndrome (PCOS) patients have an increased risk for autoimmune thyroid disease. However the mechanisms underlying this association are incompletely clarified. The aim of the present study is to evaluate the factors associated with thyroid autoimmunity in patients with PCOS. We performed a retrospective study which included 126 PCOS patients (mean age 24±4.5 years, mean body mass index, BMI 27±7.33 kg/sqm) diagnosed based on Rott...

ea0049ep1148 | Female Reproduction | ECE2017

Metformin treatment decreased free androgen index among PCOS women

Mrozinska Sandra , Wajda Anna , Czekanska Patrycja , Zdzierak Barbara , Kialka Marta , Milewicz Tomasz

: Hyperinsulinemia is one of biochemical features of polycystic ovary syndrome (PCOS). Metformin is a common drug used in PCOS. Recent studies have shown that metformin has impact on clinical and biochemical parameters in PCOS patients. The aim of our study was to assess retrospectively the influence of metformin on total testosterone, sex hormone binding globulin (SHBG) and free androgen index (FAI) in women with PCOS in polish cohort. A total of 54 patients with PCOS and irr...

ea0049ep1149 | Female Reproduction | ECE2017

Abstract withdrawn....

ea0049ep1150 | Female Reproduction | ECE2017

Biochemical and clinical characteristics of polysystic ovarian syndrome (PCOS) in women with and without type 1 diabetes (TID)

Gunness Anjuli , Pazderska Agnieska , Ahmed Mohamed , Phelan Niamh , Boran Gerard , Taylor AE , O'Reilly MW , Arlt Wiebke , Moore Kevin , Behan Lucy-Ann , Sherlock Mark , Gibney James

Abstract: PCOS prevalence is reported to be increased in reproductive-age women with type-1 diabetes (T1DM) but measurement of androgens, crucial for diagnosis, has been with inaccurate immunoassays. No studies have been reported using liquid-chromatography-mass-spectrometry (LCMS). Reproductive-age T1DM women attending a single centre were evaluated for PCOS (NIH criteria). Women with T1DM and PCOS (T1/PCOS) were compared to T1DM women without hyperandrogenism (T1/no HA), and...

ea0049ep1151 | Female Reproduction | ECE2017

A case of polycystic ovary syndrome (PCOS)

Kljajic Babic Kristina , Majic Milotic Dubravka , Peros Kristijan , Smircic Duvnjak Lea

: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in women of reproductive age. It’s a heterogeneous functional disorder of unclear etiology. The features of PCOS are disorders of ovulation, androgen excess, polycystic ovaries; it’s associated with presence of associated risk factors for cardiovascular disease (obesity, glucose intolerance, dyslipidemia). The diagnosis of PCOS is made using the Rotterdam 2003 criteria. 23yo female patie...

ea0049ep1152 | Female Reproduction | ECE2017

Does radioactive ıodine therapy affect fertility?

Evranos Ogmen Berna , Faki Sevgul , Burcak Polat Sefika , Bestepe Nagihan , Ersoy Reyhan , Cakir Bekir

Introduction: Thyroid carcinoma is common in young women. Radioactive iodine (RAI) therapy has been confirmed as a useful treatment in the management of differentiated thyroid carcinoma (DTC). For women with DTC, the effect of RAI therapy on gonadal and reproductive function is an important consideration. We aimed to evaluate effects of RAI therapy on ovarian function.Method: Women younger than 40 years old and diagnosed with thyroid cancer that required...

ea0049ep1153 | Female Reproduction | ECE2017

Androgen profiling by liquid chromatography-mass spectrometry (LC-MS) in reproductive-age women with and without diabetes

Gunness Anjuli , Pazderska Agnieska , Ahmed Mohamed , Phelan Niamh , Boran Gerard , Taylor AE , O'Reilly MW , Arlt Wiebke , Moore Kevin , Behan Lucy-Ann , Sherlock Mark , Gibney James

Abstract: The prevalence of hyperandrogenism has been reported to be increased in reproductive-age women with type-1 diabetes (T1DM). This observation however is based on findings using inaccurate immunoassays. No studies have been reported in diabetes using liquid-chromatography-mass-spectrometry (LCMS). We compared LCMS-measured androgens in T1DM-women with age-/ BMI-matched normal women, and compared findings with those in women with type-2 diabetes (T2DM) also compared to ...

ea0049ep1154 | Female Reproduction | ECE2017

Anti-mullerian hormone a marker for metformin therapy efficacy in PCO: a pilot study on Egyptian population

Sheriba Nermin , Mohamed Abdelsalam Mona , Mourad Bassem , Mamdouh Madiha , Samia Eltohamy

Background: Polycystic ovarian syndrome (PCO) is the most common endocrinopathy in adult women, and is emerging as a common cause of menstrual disturbance in the adolescent population. Insulin resistance, which is considered one of its underlying causes, has increased substantially in the past decade, putting more adolescent girls at risk for PCOS and its complications. Anti – Mullerian hormone (AMH) is secreted by the granulose...

ea0049ep1155 | Female Reproduction | ECE2017

Influence of BMI in response to ovarian stimulation and live birth in IVF

Tavares Patricia , Machado Catarina , Calejo Lucinda , Rocha Gustavo , Margarida Povoa Ana , Soares Sandra , Sousa Sonia , Joao Oliveira Maria

Introduction: The deleterious effects of obesity on spontaneous reproduction are well recognized, although the literature on obesity and assisted reproductive technology outcome remains heterogeneous and inconsistent.Objective: To estimate the effect of BMI on ovarian stimulation response and live birth in women underwent in vitro fertilization (IVF).Methods: Retrospective observational study that included women submitted ...

ea0049ep1156 | Female Reproduction | ECE2017

Anti-Mullerian hormone level is independently affected only by the ovarian factor among clinical and endocrinological factors in polycystic ovary syndrome

Matsuzaki Toshiya , Munkhzaya Munkhsaikhan , Iwasa Takeshi , Tungalagsuvd Altankhuu , Yano Kiyohito , Mayila Yiliyasi , Irahara Minoru

: Polycystic ovary syndrome (PCOS) is an endocrine disorder that affects 6–10% of women. We evaluated the correlations between the serum AMH level and various endocrine and metabolic features in PCOS. Serum AMH level was compared between 114 PCOS patient (PCOS group) and 95 normal menstrual cycle women (Control group). Correlations between serum AMH level and various endocrine and metabolic factors were analysed in PCOS group. The serum AMH level was significantly higher ...