Searchable abstracts of presentations at key conferences in endocrinology
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Society for Endocrinology BES 2018

Glasgow, UK
19 Nov 2018 - 21 Nov 2018

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The Society for Endocrinology BES will take place 19-21 Nov 2018 in Glasgow. Come and exchange knowledge, share experiences and strengthen collaborations across our global community of endocrinologists.

Oral Communications

Obesity & diabetes

ea0059oc3.1 | Obesity & diabetes | SFEBES2018

Kisspeptin stimulates insulin secretion and modulates serum metabolites in humans

Izzi Engbeaya Chioma , Comninos Alexander N , Clarke Sophie A , Jomard Anne , Yang Lisa , Jones Sophie , Abbara Ali , Narayanaswamy Shakunthala , Chia Eng Pei , Papadopoulou Deborah , Prague Julia K , Bech Paul , Godsland Ian F , Bassett Paul , Sands Caroline , Camuzeaux Stephane , Gomez Romero Maria , Pearce Jake TM , Lewis Matthew R , Holmes Elaine , Nicholson Jeremy , Tan Tricia , Ratnasabapathy Risheka , Hu Ming , Carrat Gaelle , Piemonti Lorenzo , Bugliani Marco , Marchetti Piero , Johnson Paul R , Hughes Stephen J , James Shapiro AM , Rutter Guy A , Dhillo Waljit S

Background: Limited data exists on the hormonal mediators connecting metabolism and reproduction. Animal studies show that the reproductive hormone, kisspeptin, may also be important in metabolism. We investigated the effects of kisspeptin on human metabolism for the first time, to explore possible kisspeptin-mediated links between reproduction and metabolism.Methods: We performed intravenous glucose tolerance tests (IVGTTs) in 15 healthy men (age 25&#17...

ea0059oc3.2 | Obesity & diabetes | SFEBES2018

Glucose regulates pancreatic β cell Ca2+ dynamics and connectivity in vivo in the anterior chamber of the mouse eye

Salem Victoria , Suba Kinga , Martin-Alonso Aldara , Fernando Delgadillo Silva Luis , Akhtar Nadeem , Mousavy Neda , Georgiadou Eleni , Gaboriau David , Rothery Stephen , Stylianides Theodorus , Marchett Piero , Briant Linford , Ninov Nikolay , Hodson David , Distaso Walter , Rutter Guy

Background and Aims: β cell connectivity is a feature of pancreatic islets in vitro but its existence in vivo, when innervated and continuously perfused with blood, has not yet been demonstrated. We imaged islets engrafted in the anterior chamber of the mouse eye (ACE) to explore this question.Methods: Mouse (C57BL6, Ins1Cre::GCaM6mf/f) or human islets infected with adenovirus to express GCaMP6m, were engrafted and Ca<su...

ea0059oc3.3 | Obesity &amp; diabetes | SFEBES2018

Staging of non-alcoholic fatty liver disease through LC-MS/MS analysis of the urinary steroid metabolome

Moolla Ahmad , Taylor Angela , Gilligan Lorna , Boer Jasper De , Amin Amin , Pavlov David , Hughes Beverly , Hassan-Smith Zaki , Armstrong Matt , Newsome Philip , Shah Tahir , Van Gaal Luc , Verrijken An , Francque Sven , Grove Jane , Guha Neil , Aithal Guruprasad , Barnes Ellie , Arlt Wiebke , Biehl Michael , Tomlinson Jeremy

Introduction: The development of accurate non-invasive markers to diagnose and stage non-alcoholic fatty liver disease (NAFLD) is of high importance to reduce the need for an invasive liver biopsy. These markers help to stratify patients at highest risk of hepatic and cardio-metabolic complications and allow tracking of disease progression and treatment response. We have previously described alterations in glucocorticoid metabolism that are differentially regulated across the ...

ea0059oc3.4 | Obesity &amp; diabetes | SFEBES2018

Knockout of glucocorticoid receptor on AgRP/NPY/GABA (ANG) neurons identifies a potential role for this neuronal population in mediating glucocorticoid–induced insulin resistance in female mice

Harno Erika , Davies Alison , Allen Tiffany-Jayne , Sefton Charlotte , Wray Jonathan R , Coll Anthony P , White Anne

Glucocorticoids (Gcs) are used in the treatment of inflammatory disorders including asthma and rheumatoid arthritis. However, long-term use can cause metabolic side-effects including obesity and diabetes. Previous studies have shown that Gcs increase Agrp expression and that AgRP/NPY/GABA (ANG) neurons can regulate appetite and insulin sensitivity. To investigate the effects of chronic Gc treatment directly on ANG neurons, we crossed AgRP-IRES-Cre with GR...

ea0059oc3.5 | Obesity &amp; diabetes | SFEBES2018

Hypothalamic arcuate glucokinase and its downstream pathways are critical in glucose homeostasis

Ratnasabapathy Risheka , Ma Yue , Izzi-Engbeaya Chioma , Nguyen-Tu Marie-Sophie , Richardson Errol , Hussain Sufyan , De Backer Ivan , Holton Christopher , Norton Mariana , Carrat Gaelle , Schwappach Blanche , Rutter Guy A , Dhillo Waljit S , Gardiner James V

As the nation gets fatter, the incidence of diabetes is also rising. The brain is now emerging as a critical mediator of blood sugar control, re-directing focus away from the traditional pancreas-centred model. The enzyme glucokinase (GK) acts as a glucose sensor in many tissues including glucose-sensitive neurones within the hypothalamic arcuate nucleus. However, the role of GK here is unclear. We investigated the role of arcuate GK in glucose homeostasis in both healthy and ...

ea0059oc3.6 | Obesity &amp; diabetes | SFEBES2018

5β-reductase (AKR1D1) is a potent regulator of hepatic insulin sensitivity, carbohydrate and lipid metabolism in vitro and in vivo

Nikolaou Nikolaos , Gathercole Laura , Marchand Lea , Althari Sara , Green Charlotte , McNeil Catriona , Harris Shelley , van de Bunt Martijn , Arlt Wiebke , Hodson Leanne , Tomlinson Jeremy

Steroid hormones and BAs are established regulators of metabolic phenotype. 5β-reductase (AKR1D1) is highly expressed in the liver where it inactivates steroid hormones and catalyses a fundamental step in bile acid (BA) synthesis. We have hypothesised that AKR1D1 plays a crucial regulatory role in hepatic metabolic homeostasis. Genetic manipulation of AKR1D1 was performed in human liver HepG2 and Huh7 cells. Expression changes were confirmed by qPCR and western blotting, ...