Endocrine Abstracts

Background

Oxidative stress in hyperlipidemic conditions can affect superoxide dismutase (SOD), a natural antioxidant in the body. One of the organs affected by increased oxidative stress due to hyperlipidemic conditions is the testicular organ. Increased oxidative stress in the testicular organ can damage to sperm cell and increase the risk of infertility. Soy milk is a beverage product made from soybeans which are widely consumed as an alternative to cow’s milk. Fermented soy products can improve nutritional quality and have beneficial effects on health.

Aim

The aim of this study was to determine SOD inhibition rate in the testicular organs of hyperlipidemic rat after intervention with fermented soy milk.

Method

The subjects are three-month-old rats (Rattus norvegicus) male Wistar strain with body weight 200–300 grams divided into 3 groups (Negative Control (NC), Positive Control (PC), and Intervention group). PC and intervention group were given quail egg yolk for 2 weeks with a dose of 5 ml for hyperlipidemic induction while group of NC were only given fed ad libitum. After hyperlipidemic induction, the intervention group was given fermented soy milk (5 ml) from Lactobacillus bulgaricus and soybean. All rats then terminated to taken the testicular organ to measure the level of SOD inhibition rate. All data were statistically analyzed with One-Way ANOVA and Bonferroni Post-Hoc test. Values were considered significant at P<0.05.

Results

Mean of SOD inhibition rate (%) in rats was 82.03±1.62 in NC group, 17.97±2.06 in PC group, and 70.31±2.38 in intervention group. The One-Way ANOVA test showed significant differences activity between group with P <0.001 and the Bonferroni Post-Hoc test P <0.001.

Conclusion

Intervention with fermented soy milk can increase superoxide dismutase inhibition rate in the testicular organs of hyperlipidemic rats.

Keywords: fermentation food, hyperlipidemia, soy milk, superoxide dismutase, testiscular organ.