Searchable abstracts of presentations at key conferences in endocrinology
Endocrine Abstracts (2021) 73 PEP3.3 | DOI: 10.1530/endoabs.73.PEP3.3

ECE2021 Presented Eposters Presented ePosters 3: Pituitary and Neuroendocrinology (8 abstracts)

Insulinoma-associated protein 1 (INSM1) may serve as a sensitive and specific immunohistochemical and molecular biomarker of neuroendocrine differentiation in pancreatic neuroendocrine tumors (PNETs)

Darko Katalinic 1 , Ivan Aleric 1 , Aleksandar Vcev 1 & Elke Kattner 2

1Faculty of Dental Medicine and Health and Faculty of Medicine, Department of Clinical Medicine and Department of Internal Medicine, Osijek, Croatia; 2Herford Teaching Hospital, Department of Hematology and Medical Oncology, Herford, Germany


Insulinoma-associated-1 (INSM1), encoded by the INSM1 gene is key factor in pancreatic endocrine, sympatho-adrenal and pan-neurogenic development. It has also been identified in multiple tumors of neuroendocrine origin but not thoroughly investigated as a potential neoplastic biomarker. The aim of the study was to evaluated INSM1 as a semiquantitative immunohistochemical biomarker as well as quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) biomarker for pancreatic neuroendocrine tumors (PNETs).

Material and Methods

Metastatic PNETs (liver metastases) specimens (n = 24) matched control (normal) tissue were surgically collected and INSM1 expression for tumor and control tissue was analysed by immunohistochemistry. Additionally, mRNA expression was determined by qRT-PCR.


INSM1 expression was significantly increased in neoplastic vs nonneoplastic tissue. Using immunohistrochemistry, we found that INSM1 was not detected in any control, nonneoplastic, non-neuroendocrine tissue. In PNETs tissue, INSM1 was detectable by immunohistochemistry and qRT-PCR in 22 of total 24 specimens (91.66%). Compared to normal tissue specimens, the INSM1 showed significant overexpression in metastatic PNETs.


Our findings highlight that INSM1 may serve as a promissing, novel, neuroendocrine, immunohistochemical and molecular biomarker in patient diagnosed with PNETs.

Volume 73

European Congress of Endocrinology 2021

22 May 2021 - 26 May 2021

European Society of Endocrinology 

Browse other volumes

Article tools

My recent searches

No recent searches.