Endocrine Abstracts

Mitochondria play an important role in generating energy, and they are essential to cell survival. Mitochondria have varieties of functions, such as regulating intracellular calcium concentration and signal transduction, controlling hormone synthesis, inflammatory responses, and free radicals. In particular, this research is interested in the mitochondria in kidney tubular cell. Since mitochondria in tubular cells play an important role in supplying energy, removal of waste and reabsorption of nutrients in the blood, control of blood pressure, and maintenance of homeostasis. Mitochondrial damage may occur by drugs or related chemicals for the treatment of various diseases. Therefore, it may be related to mitochondrial dysfunction. Following experiment was conducted to investigate the relation between kidney and mitochondrial dysfunction. First, five drugs known to be toxic and two non-toxic chemicals to mitochondria were selected. After treatment with mouse kidney stem cells, the specific concentration of cell viability (IC₅₀) was selected for each drug. The results of doxorubicin, a representative mitochondria toxicant, treated on the cell, confirmed that the expression of Mn-SOD increased according to the increasing concentration of doxorubicin. Result of performing an analysis using MitoSOX red staining to confirm the relationship between mitochondrial toxicity and reactive oxygen species (ROS), it was found that the level of ROS according to the concentration of a toxic drug was relatively increased compared to that of a non-toxic drug.

Acknowledgement: This research was supported by grants (20183MFDS525) from the Ministry of Food and Drug Safety in 2021.