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Endocrine Abstracts (2023) 90 P35 | DOI: 10.1530/endoabs.90.P35

ECE2023 Poster Presentations Calcium and Bone (83 abstracts)

Impact of a single oral bolus of 120,000 IU of cholecalciferol on vitamin D metabolites in the elderly: the results of randomized open study

Dorota Leszczynska 1 , Alicja Szatko 1 , Konrad Kowalski 2 , Dorota Raczkiewicz 3 , Magdalena Ostrowska 1 , Waldemar Misiorowski 1 , Wojciech Zgliczyński 1 & Piotr Glinicki 1

1Centre of Postgraduate Medical Education, Department of Endocrinology, Warsaw, Poland; 2Masdiag Laboratory, Warsaw, Poland; 3Centre of Postgraduate Medical Education, Department of Medical Statistics, School of Public Health, Warsaw, Poland

Background: Vitamin D is a dietary micronutrient responsible for calcium and phosphorus metabolism and multiple extraskeletal actions. The assessment of vitamin D status is commonly based on measurement of 25(OH)D total concentration in serum. However, the usage of LC-MS/MS analytical technique allows to reliably assess a panel of vitamin D metabolites in serum or plasma, which may help to investigate the metabolic paths of vitamin D, especially in populations at risk of deficiency.

Methods: A randomized, two-arms, open study was conducted on 58 patients (28 female and 30 male; aged from 61 to 96 years old). The primary aim was to assess the effects of a single, high, oral dose of vitamin D3 (120.000 IU) on serum 25(OH)D3, 25(OH)D2, 24,25(OH)2D3, 3-epi-25(OH)D3 and 1,25(OH)2D3 concentrations (measured by LC-MS/MS), calculated 24,25(OH)2D3/25(OH)D3 ratio and 25(OH)D3/3-epi-25(OH)D3 ratio at baseline, 3 days and 7 days after administration, compared to control group. The secondary aim was assessment of influence of percentage of fat tissue on serum metabolites of vitamin D and their changes after bolus dose.

Results: 56.6% study group attained a serum 25(OH)D3 concentration >30 ng/ml. All subjects, except for one patient achieved a serum 25(OH)D3 concentration >20 ng/ml after administration. No one exceed reference value of vitamin D (30–50 ng/ml). Among participants who received vitamin D3 there were significant increase in 25(OH)D3, 3-epi-25(OH)D3, 1,25(OH)2D3, 24,25(OH)2D3 on 3rd day after administration. 24,25(OH)2D3 concentration gradually grew, achieving the highest concentration on 7th day. The percentage increase of 25(OH)D3 was negatively correlated with baseline 25(OH)D3 (r=-0.688, P = 0.001). Positive correlation between percentage increase in 25(OH)D3 and a percentage increase serum concentration of 24,25(OH)2D3 (r=0.954, P< 0.001), 3-epi-25(OH)D3 (r=8.03, P<0.001) and 1,25(OH)2D3 (r=0.789, P<0.001) were found. None of the study participants developed hypercalcemia. The baseline concentration of analyzed metabolites of vitamin D in serum and their percentage increase were neither dependent on BMI nor percentage of fat tissue.

Conclusions: High dose of vitamin D rapidly increases 25(OH)D3 concentration in the elderly patients. The response to the bolus of vitamin D includes activation of 3-epimerase, followed by production of 24,25(OH)2D3, which protects from excessive increase of active form of vitamin D.

Volume 90

25th European Congress of Endocrinology

Istanbul, Turkey
13 May 2023 - 16 May 2023

European Society of Endocrinology 

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