Searchable abstracts of presentations at key conferences in endocrinology

ea0059p109 | Diabetes & cardiovascular | SFEBES2018

Activation of the adhesion GPCR GPR56 by a synthetic tethered agonist improvesislet β-cell function

Olaniru Oladapo Edward , Atanes Patricio , Persaud Shanta

GPR56 is an adhesion G-protein coupled receptor (GPCR), which we have shown to be the most abundant GPCR in mouse and human islets. The extracellular N-terminal domain of adhesion GPCRs contains a tethered agonist, buried within the GPCR autoproteolysis-inducing domain. Synthetic peptides mimicking tethered agonist sequences can activate a variety of adhesion GPCRs including GPR56. Here we investigated the effect of a GPR56 tethered agonist peptide, P7, on β-cell function...

ea0077p177 | Metabolism, Obesity and Diabetes | SFEBES2021

Obesity-induced upregulation of chemokine Ccl4 in mouse visceral adipose tissue: effects on β-cell function

Ashik Tanyel , Atanes Patricio , Lee Vivian , Persaud Shanta

Introduction: Adipose tissue-derived peptides, known as adipokines, act as key regulators of metabolic homeostasis, but little information is available on adipokine-mediated cross-talk with β-cells via islet GPCR interactions, nor whether this is altered in obesity. The expression profile of islet GPCR peptide ligand mRNAs in visceral adipose tissue from lean and diet-induced obese mice was therefore defined and the functional effects of Ccl4 on β-cells were characte...

ea0065p166 | Metabolism and Obesity | SFEBES2019

Obesity-induced changes in hepatocyte and skeletal myocyte expression of mRNAs encoding islet GPCR peptide ligands

Atanes Patricio , Smith Lorna , Bowe James , Persaud Shanta

Introduction: Insulin-sensitive tissues such as liver and skeletal muscle modify their gene expression under conditions of obesity-induced insulin resistance, and some of these gene products may be released to maintain glucose homeostasis. This study aimed to identify mRNAs encoding liver and skeletal muscle peptides that have the potential to regulate β-cell function by binding to islet GPCRs, and to quantify changes in expression of these liver and muscle mRNAs in obese...

ea0077p188 | Metabolism, Obesity and Diabetes | SFEBES2021

Exploring the translational potential of the NPY Y4 receptor for treating Type 1 Diabetes

Haq Naila , Toczyska Klaudia , Olaniru Oladapo , Atanes Patricio , Beck-Sickinger Annette , Bewick Gavin

Type 1 diabetes (T1D) is an autoimmune, heterogenous disease caused by immune-mediated destruction of insulin-producing β-cells in the pancreas. The only approved treatment strategies are exogenous insulin replacement therapy and islet transplantation. Leading experimental approaches have focussed on suppression and/or modulation of the immune system. However, efforts to increase β-cell survival are also of great interest. Recent studies in our lab have identified ne...

ea0065p234 | Metabolism and Obesity | SFEBES2019

Adhesion G-protein coupled receptors as novel players in islet development

Olaniru Oladapo Edward , Atanes Patricio , Toczyska Klaudia , Piao Xianhua , Persaud Shanta

Adhesion GPCRs (aGPCRs) have been implicated in developmental processes and deletion of some aGPCRs in neonatal mice results in decreased β-cell differentiation capacity, leading to glucose intolerance in adult life. Here, we investigated the expression of aGPCR mRNAs in mouse islets and determined the expression and function of GPR56, the most abundant aGPCR in islets, in developing mouse pancreas. Quantitative PCR indicated that mouse islets expressed mRNAs encoding 26 ...