Searchable abstracts of presentations at key conferences in endocrinology

ea0022p51 | Adrenal | ECE2010

In vitro effect of serotonin (5-HT) on cortisol secretion in primary pigmented nodular adrenal disease

Louiset Estelle , Duparc Celine , Perraudin Veronique , Renouf Sylvie , Libe Rossella , Stratakis Constantine A , Bertherat Jerome , Lefebvre Herve

Illegitimate membrane receptors are known to play a role in cortisol secretion in adrenal adenomas and ACTH-independent macronodular adrenal hyperplasia (AIMAH) causing Cushing’s syndrome. Conversely, illegitimate receptors have never been described in primary pigmented nodular adrenal disease (PPNAD). In the normal adrenal gland, serotonin (5-HT) has been shown to stimulate cortisol secretion through activation of 5-HT receptor type 4 (5-HT4) whereas, in some AIMAH tissu...

ea0016p60 | Adrenal | ECE2008

Intraadrenal production of ACTH in macronodular bilateral adrenal hyperplasia causing Cushing's syndrome: its role in the physiopathology of hypercortisolism

Louiset Estelle , Duparc Celine , Groussin Lionel , Bertherat Jerome , Bertagna Xavier , Kuhn Jean-Marc , Lefebvre Herve

Illicit expression of membrane receptors for circulating regulatory factors, such as GIP and LH receptors, has been well documented in ACTH-independent macronodular adrenal hyperplasias (AIMAHs) causing Cushing’s syndrome. In addition, we have observed an abnormal expression of serotonin, arginine vasopressin and ACTH in a subpopulation of steroidogenic cells in two AIMAH tissues. The aim of the present study was: (i) to investigate the presence of ACTH by immunohistochem...

ea0099p359 | Reproductive and Developmental Endocrinology | ECE2024

Expression of the serotonin signaling pathway in a testicular adrenal rest tumor in a patient with 21-hydroxylase deficiency

Menager Lola , Rives-Feraille Aurelie , Moutard Laura , Rives Nathalie , Lefebvre Herve , Louiset Estelle

Testicular adrenal rest tumors (TART) are frequently detected in male patients with 21-hydroxylase (21-OH) deficiency. Increase in plasma ACTH levels, due to cortisol deficit, is responsible for TART growth. Indeed, glucocorticoid substitution reduces both plasma ACTH and TART development in the majority of patients. Nevertheless, some patients with TART are resistant to the glucocorticoid treatment, suggesting the existence of other factors stimulating TART growth. The aim of...

ea0026p16 | Adrenal cortex | ECE2011

Potential role of mast cells in the physiopathology of aldosterone-producing adenoma

Duparc Celine , Moreau Lucile , Perraudin Veronique , Gobet Francoise , Meatchi Tchao , Plouin Pierre-Francois , Zennaro Maria-Christina , Louiset Estelle , Lefebvre Herve

We have shown that 5-hydroxytryptamine (5-HT) receptor type 4 (5-HT4R) agonists stimulate aldosterone production in patients with aldosterone-producing adenoma (APA). Moreover, 5-HT-positive cells were observed in APA tissues and 5-HT4R mRNAs were markedly overexpressed in APAs in comparison with normal adrenals. All these results suggested that 5-HT produced by adrenal mast cells could be involved in the physiopathology of APAs. The aim of this work was to investigate the rol...

ea0016p61 | Adrenal | ECE2008

A case of renin-producing adrenocortical carcinoma with Cushing's syndrome: in vitro effect of serotonin on renin and cortisol secretions

Louiset Estelle , Isvi Karen , Gasc Jean-Marie , Duparc Celine , Cauliez Bruno , Laquerriere Annie , Kuhn Jean-Marc , Lefebvre Herve

A 48-year-old woman was referred for ACTH-independent Cushing’s syndrome associated with a left adrenal tumor. Contrasting with hypercortisolism-related hypertension and hypokalemia, renin and aldosterone levels were paradoxically increased. The tumor was surgically removed and pathological examination of the tissue established the diagnosis of corticosurrenaloma. Fragments of the tumor were obtained for in situ hybridization, immunohistochemical and cell incubatio...

ea0099ep13 | Adrenal and Cardiovascular Endocrinology | ECE2024

Implication of serotonin in the physiopathology of aldosterone-producing adenoma

Duparc Celine , D'Agostino Margot , Lopez Antoine-Guy , Renouf Sylvie , Manceau Gilles , Meatchi Tchao , Amar Laurence , Louiset Estelle , Lefebvre Herve

In the human adrenal gland, serotonin (5-HT), released by subcapsular mast cells strongly stimulates aldosterone production via activation of the type 4 serotonin receptor (5-HT4R). Consistently, plasma aldosterone levels increase after administration of 5-HT4R agonists like cisapride to healthy volunteers. Previous studies performed in aldosterone producing adenomas (APA) have shown an increase in mast cells density and an overexpression of HTR4 gene, encoding 5-HT4R...

ea0090s7.3 | Aberrant/illicit expression of receptors in adrenal lesions | ECE2023

LHCG Receptor in pheochromocytoma

Lopez Antoine-Guy , Duparc Celine , Renouf Sylvie , Sabourin Jean-Christophe , Buffet Alexandre , Gimenez-Roqueplo Anne-Paule , Dubessy Christophe , Louiset Estelle , Lefebvre Herve

Pheochromocytomas/paragangliomas (PPGL) are catecholamine-producing neuroendocrine tumors that account for less than 1% of all hypertension cases. Despite their rarity, these tumors must be early detected and treated to prevent potentially life-threatening adrenergic crises. Unfortunately, PPGL are frequently unrecognized during pregnancy, leading to a high risk of either maternal or fetal complications. In return, pregnancy can trigger catecholamine secretory discharges in pa...

ea0063p814 | Adrenal and Neuroendocrine Tumours 3 | ECE2019

Abnormal serotonin regulatory loop in adrenals of patients with Cushing’s syndrome and 21-hydroxylase deficiency

Le Mestre Julie , Duparc Celine , Reznik Yves , Bonnet-Serrano Fideline , Touraine Philippe , Chabre Olivier , Young Jacques , Sibony Mathilde , Gobet Francoise , Raverot Gerald , Bertherat Jerome , Louiset Estelle , Lefebvre Herve

In the human adrenal gland, serotonin (5-HT), released by subcapsular mast cells stimulates corticosteroid secretion through activation of type 4 serotonin receptors (5-HT4R) positively coupled to cAMP/proteine kinase A (PKA) signaling pathway and calcium influx. The 5-HT4R is principally expressed in zona glomerulosa cells explaining why 5-HT strongly stimulates aldosterone production but only exerts a modest stimulatory action on cortisol. Interestingly, in primary pigmented...

ea0049gp12 | Adrenal 2 | ECE2017

Activation of the cAMP/PKA transduction system triggers abnormal expression of the serotonin signaling pathway in human adrenocortical cells

Mestre Julie Le , Duparc Celine , Bram Zakariae , Reznik Yves , Bertherat Jerome , Touraine Philippe , Young Jacques , Chabre Olivier , Stratakis Constantine A. , Lefebvre Herve , Louiset Estelle

In human adrenals, serotonin (5-HT), released by subcapsular mast cells, increases aldosterone secretion through activation of type 4 serotonin receptors (5-HT4R) but only exerts a modest control on cortisol production. Interestingly, illicit synthesis of 5-HT in adrenocortical cells as well as overexpression of the 5-HT4R and ectopic expression of the type 7 receptor (5-HT7R) have been observed in bilateral macronodular adrenal hyperplasia res...

ea0037ep1214 | Clinical Cases–Pituitary/Adrenal | ECE2015

Aberrant expression of serotonin receptors in an aldosterone- and cortisol-producing adenoma

Lytrivi Maria , Brisbois Denis , Corvilain Bernard , Demetter Pieter , Garcia Camilo , Lucidi Valerio , Moreno-Reyes Rodrigo , Vudu Stella , Duparc Celine , Wils Julien , Lefebvre Herve , Louiset Estelle , Driessens Natacha

Introduction: Aberrant expression of serotonin receptors has been described to be involved in the pathophysiology of both aldosterone-producing and cortisol-producing adrenal adenomas.Case report: A 46-year-old woman was referred for evaluation of severe hypertension associated with hypokalaemia. Primary aldosteronism with concurrent subclinical Cushing’s syndrome was diagnosed. A CT-scan identified a lesion of 4 cm in the right adrenal gland and a ...