Searchable abstracts of presentations at key conferences in endocrinology

ea0035p827 | Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2014

Effect of ubiquitin/proteosome inhibition on acth secretion by rat corticotropes

Sesta Antonella , Cassarino Maria Francesca , Cavagnini Francesco , Giraldi Francesca Pecori

ACTH is the product of proteolytic processing of its precursor, proopiomelanocortin (POMC) and, in turn, is subject to proteolytic degradation. Regulation of proteolysis is crucial to several intracellular processes, including protein activation and inactivation, and thus contributes to active peptide concentrations. The proteosome 26S/ubiquitin system (UPS) is the main, non-lisosomial intracellular proteolytic pathway and alterations to this system have been observed in both ...

ea0037ep15 | Adrenal cortex | ECE2015

The effect of retinoic acid on human adrenal corticosteroid secretion in vitro

Sesta Antonella , Tapella Laura , Cassarino Maria Francesca , Castelli Luigi , Cavagnini Francesco , Giraldi Francesca Pecori

Retinoic acid, a derivative of vitamin A, has recently yielded promising results in the treatment of Cushing’s disease (Pecori Giraldi et al. JCEM 2012). Its main site of action appears to be the tumoural corticotrope as retinoic acid inhibits POMC transcription and corticotrope proliferation (Paez-Pereda et al. JCI 2001). Studies on tumoural adrenal cell lines have revealed an additional inhibitory effect on cell proliferation and stimulated co...

ea0037ep657 | Pituitary: basic and neuroendocrinology | ECE2015

Effect of phenols and phthalates on rat and human pituitary cell proliferation

Tapella Laura , Sesta Antonella , Losa Marco , Cavagnini Francesco , Giraldi Francesca Pecori

An increased prevalence of acromegaly and pituitary tumors has recently been observed in high industrial density areas (Cannavò et al. J Endocrinol 2010) or after dioxin exposure (Pesatori et al. Eur J Endocrinol 2008). These data as well as vitro studies on endocrine disruptors (EDs) suggest that environmental pollutants may affect hormonal secretion and pituitary cell proliferation (Blake et al. Proc Soc Exp Biol...

ea0022p182 | Clinical case reports and clinical practice | ECE2010

Occult ACTH-secreting pheochromocytoma

De Martin Martina , Giraldi Francesca Pecori , Pagliardini Luca , Cassarino Francesca , Ambrogio Alberto G , Cavagnini Francesco

A 69-year-old woman developed hypertension, polyuria and hyperglycemia over the course of several months. The initial biochemical investigation revealed ACTH-dependent hypercortisolism (UFC 409.7 μg/24 h, NR 10–80; ACTH 43.3 pg/ml, NR 8–50; OST 28 μg/dl) and moderately elevated urinary catecholamines (E 80.5 μg/24 h, NR 2.5–33.6; norE 235.8 μg/24 h, NR 18.1–128.2). Further investigations disclosed absent ACTH and cortisol responses to CR...

ea0022p562 | Neuroendocrinology and Pituitary (<emphasis role="italic">Generously supported by Novartis</emphasis>) | ECE2010

Assessment of biochemical control of acromegaly during treatment with somatostatin analogues: is there still a role for glucose oral load?

Carzaniga Chiara , Scacchi Massimo , Vitale Giovanni , Fatti Maria Letizia , Giraldi Francesca Pecori , Andrioli Massimiliano , Cavagnini Francesco

Biochemical control of acromegaly is currently defined by the achievement of GH suppression after oral glucose tolerance test (OGTT) and of normal age- and gender-matched IGF1 levels. OGTT is believed to inhibit somatotropin secretion by enhancing central somatostatinergic tone; thus, the use of this test in evaluating biochemical control in acromegalic patients on somatostatin analogues (SSA) is questionable. To gain further insights into this topic, we analyzed basal and nad...

ea0020p39 | Adrenal | ECE2009

Anemia in male patients with cushing’s syndrome before and after cure

Giraldi Francesca Pecori , Ascoli Paola , De Martin Martina , Polli Nicoletta , Cavagnini Francesco

Glucocorticoids are known to exert a stimulatory action on white blood cell precursor proliferation but little is known on erythrocyte counts in patients with Cushing’s syndrome.Aim: Of the present study was to assess red and white blood cell parametres in a large series of patients with Cushing’s syndrome and follow the blood cell changes after postsurgical remission or persistence of hypercortisolism.Methods: Of 84 pati...

ea0016p108 | Clinical cases | ECE2008

Prolonged QT interval on the electrocardiogram in hypogonadal men

Filippini Barbara , Giraldi Francesca Pecori , Toja Paola Maria , Michalidis Jorgo , Badiale Marco Stramba , Cavagnini Francesco

Background: QT interval reflects cardiac ventricular repolarization and, if prolonged, increases the risk of malignant arrhythmias such as torsade de pointes. QT interval duration is similar in boys and girls during childhood but shortens in males after puberty. In fact, reference limits for QTc in adults reflect this difference, i.e. lower than 460 ms in women and lower than 440 ms in men. Experimental studies suggest that testosterone is the major contributor to shortening o...

ea0016p429 | Neuroendocrinology | ECE2008

Variable effects of preproTRH(178–199) on ACTH secretion by human corticotrope tumors

Giraldi Francesca Pecori , Pesce Samantha , Maroni Paola , Losa Marco , Cavagnini Francesco

PreproTRH (178–199), a 22-aminoacid cleavage product of the TRH prohormone, has been postulated to act as an ACTH-releasing inhibitor. Indeed, in vitro evidence indicates that this peptide may inhibit basal and stimulated ACTH secretion in rodent anterior pituitary primary cultures and cell lines (Redei 1995, Revskoy 2001), although not all studies concur (Nicholson 1996). Aim of the present study was to test the effect of preproTRH(178–199) in human tumoral c...

ea0073aep15 | Adrenal and Cardiovascular Endocrinology | ECE2021

Effect of retinoic acid on adrenal primary cultures from patients with Cushing’s disease

Giraldi Francesca Pecori , Sesta Antonella , Tapella Laura , Cassarino Maria Francesca , Castelli Luigi

Retinoic acid, a major modulator of adrenal development and differentiation, has also been shown to inhibit ACTH secretion by tumoral corticotropes. Recent clinical trials in patients with Cushing’s disease revealed that retinoic acid exerts beneficial effects in these patients (1, 2). Of note, the decrease in cortisol secretion during retinoic acid administration was more pronounced than the change in ACTH levels (1) suggesting a direct action at adrenal level. Aim of th...

ea0041ep857 | Pituitary - Basic | ECE2016

Combined clinical and gene expression profiling in human ACTH-secreting pituitary tumors

Cassarino Maria Francesca , Cassarino Andrea , Terreni Mariarosa , Ambrogio Alberto Giacinto , Gentilini Davide , Losa Marco , Cavagnini Francesco , Giraldi Francesca Pecori

Introduction: We have previously reported on the considerable variability in ACTH-secreting pituitary adenomas in terms of responses to major modulators in vitro (Pecori Giraldi et al J. Neuroendocrinol. 2011). Further studies revealed also differences in gene expression profiles in specimens analyzed by microarray analysis. Aim of this study is to correlate transcriptome expression pattern in archival human ACTH-secreting adenomas with clinical features of patients p...