Searchable abstracts of presentations at key conferences in endocrinology

ea0014p273 | (1) | ECE2007

Gs-dependent receptor endocytosis of melanocortin-4 receptors

Breit Andreas , Heling Dominik , Gudermann Thomas

Melanocortin receptors (MCR), which belong to the superfamiliy of G protein-coupled receptors (GPCR), are preferentially coupled to Gs proteins and play a major role in the regulation of energy homeostasis. In line with this notion, mutations in the MC4R gene are the most frequent monogenic cause of severe obesity in human beings. Recently it has been shown that the MC4R receptor undergoes, similar to most GPCR, GPCR kinase (GRK) and arrestin-mediated ligand-promoted receptor ...

ea0014p332 | (1) | ECE2007

Gq/11-dependent signaling of the tyhrotropin receptor regulates metallothionein 1 expression in human thyroid carcinoma cells

Buech Thomas , Baeck Christer , Nowak Nicola , Gudermann Thomas

Metallothioneins (MT) are cystein-rich intracellular proteins which exert anti-apoptotic effects by protecting cells against oxidative stress and DNA damage. Previously, expression of MT in normal and neoplastic thyroid tissue has been demonstrated. However, the thyroidal regulation of MT expression is widely unsettled. Thus, we investigated the expression of MT isoform 1 in human thyroid carcinoma cells (FTC-133-TSHR) upon stimulation with thyrotropin (TSH). Using quantitativ...

ea0016p336 | Endocrine tumours | ECE2008

G12/13-dependent growth of small cell lung cancer cells in vitro and in vivo

Buch Thomas , Grzelinski Marius , Pinkenburg Olaf , Gold Maike , Aigner Achim , Gudermann Thomas

The malignant phenotype of small cell lung cancer (SCLC) cells critically relies on the autocrine stimulation of G12/13- and Gq/11-coupled neuropeptide receptors. By this means, G12/13- and Gq/11-dependent signalling pathways are constitutively activated in these cells. While Gq/11-dependent signalling has been shown to promote proliferation of SCLC cells, the role of signalling pathways via G12/13 is still elus...

ea0014p636 | (1) | ECE2007

The multi-PDZ domain protein MUPP1: a scaffolding protein controlling the acrosome reaction in mammalian spermatozoa

Ackermann Frauke , Meyer Dorke , Heydecke Diana , Wilhelm Beate , Gudermann Thomas , Boekhoff Ingrid

Upon adhesion to the zona pellucida, mammalian sperm undergo regulated exocytosis of the acrosome. Despite the difference in size, some parallels can be drawn concerning the signal transduction processes controlling the sperm acrosome reaction and synaptic vesicle exocytosis. Since components of signal transduction pathways are often organized in multiprotein signalling complexes, attempts were made to identify scaffolding proteins expressed in the acrosomal region of mammalia...

ea0014p637 | (1) | ECE2007

Expression of the G-protein α-subunit gustducin in mammalian spermatozoa

Meyer Dorke , Fehr Johanna , Borth Heike , Widmayer Patricia , Wilhelm Beate , Gudermann Thomas , Boekhoff Ingrid

The G protein subunit α-gustducin is generally accepted as a marker for chemosensitive cells. Since chemosensation is especially important for the navigation of sperm towards the egg, attempts were made to explore whether α-gustducin might also be expressed in spermatozoa. RT-PCR experiments revealed that a gustducin PCR specific RNA fragment with the predicted size could be amplified from total mouse and rat testis. To identify the testicular cell type in which &#94...

ea0070aep1002 | Hot topics (including COVID-19) | ECE2020

Intact endothelial epoxyeicosatrienoic acids pathway in primary aldosteronism – the route to new treatment strategies?

Fruehbuss Laura , Meng Yao , Sun Jing , Gonzalez Marques Jair , Koletzko Berthold , Mederos Y Schnitzler Michael , Gudermann Thomas , Beuschlein Felix , Ann Williams Tracy , Heinrich Daniel , Adolf Christian , Reincke Martin , Schneider Holger

Rationale: Endothelial dysfunction (ED) is a hallmark of primary aldosteronism and paves the way for subsequent atherosclerotic disease. Past research has confirmed that one factor involved in ED is disturbed nitric oxide (NO) signalling. Since defects in NO release alone cannot explain the whole effect, we set out to address the role of endothelial CYP-expoygenase products (epoxyeicosatrienoic acids, EETs) in aldosterone-mediated endothelial dysfunction.<p class="abstext"...