Searchable abstracts of presentations at key conferences in endocrinology

ea0065p243 | Metabolism and Obesity | SFEBES2019

Mass spectrometric characterisation of circulating proinsulin-derived peptides in insulin autoimmune syndrome

Church David , Halsall David , Gribble Fiona , Reimann Frank , Semple Robert , Kay Richard

Autoimmune immunoglobulins directed against peptide hormones are well-described. These are often clinically benign, but may cause a deleterious effect on the accuracy of clinical immunoassays. A notable exception is Insulin Autoimmune Syndrome (IAS, also known as Hirata disease), where antibody-binding has a direct effect on insulin kinetics resulting in aberrant glucose control. Current methods to diagnose IAS rely on crude immune-subtraction techniques such as polyethylene g...

ea0025oc3.1 | Pituitary and thyroid | SFEBES2011

Development of a novel mass spectroscopy-based method for determining serum IGF1: assessment in a cohort of newly diagnosed subjects with acromegaly

Halsall David , Kay Richard , Taylor Kevin , Annamalai Anand , Kandasamy Narayanan , Wark Gwen , Pleasance Steve , Gurnell Mark

Background: The recently published ‘Consensus on Criteria for Cure of Acromegaly’ (Giustina et al. JCEM, 2010) highlighted concerns regarding the quality of currently available insulin-like growth factor 1 (IGF1) immunoassays which may contribute, at least in part, to the discordance between GH and IGF1 that is observed in up to 30% of patients with acromegaly after treatment. The development of mass spectroscopy (MS)-based technology has been proposed ...

ea0086oc5.6 | Metabolism, Obesity and Diabetes | SFEBES2022

Labelling and characterisation of somatostatin secreting D-Cells in primary human duodenal organoids culture

Bany Bakar Rula , Sayda Mariwan H. , Smith Christopher A. , Guccio Nunzio , Kay Richard , Gribble Fiona M. , Reimann Frank

Backgrounds and aims: Enteroendocrine cells (EECs) are hormone-secreting cells within the intestinal epithelium that play an important role in regulating food absorption, insulin secretion and appetite. The somatostatin (SST)-producing D-cell is an EEC of particular interest due to the profound inhibition exerted by SST over other EECs, highlighting D-cells as critical regulator of the enteroendocrine axis. The aim of this study was to profile the transcriptom...

ea0077oc3.6 | Metabolism, Obesity and Diabetes | SFEBES2021

Stimulation of motilin secretion by bile, free fatty acids and acidification in human duodenal organoids

Miedzybrodzka Emily L , Foreman Rachel E , Lu Van B , George Amy L , Smith Christopher A , Larraufie Pierre , Kay Richard G , Goldspink Deborah A , Reimann Frank , Gribble Fiona M

Objective: Motilin is a proximal small intestinal hormone with roles in gastrointestinal motility, gallbladder emptying and hunger initiation. The molecular mechanisms underlying motilin release in response to fats, bile and duodenal acidification are poorly understood, in part due a lack of suitable cellular and rodent models. We therefore generated a novel human intestinal organoid model with fluorescently labelled motilin-expressing M-cells, which we used to establish the k...