Searchable abstracts of presentations at key conferences in endocrinology

ea0026p124 | Female reproduction | ECE2011

Two hormone for one receptors: dissecting out LH and hCG activity with an in vitro approach.

Casarini Livio , La Marca Antonio , Pignatti Elisa , Simoni Manuela

Introduction: LH and hCG act on the same receptor (LHCGR), have different half-lives and in vivo biopotency. It is not known whether they elicit the same cellular and molecular response. The aim of this study was to compare the kinetics of cAMP response to recombinant LH and hCG.Design: In COS-7 cells permanently expressing the human LHCGR (COS-7/LHCGR) we evaluated LH and hCG dose-response curves, by measuring total cAMP after 3 h of incubation. ...

ea0032p586 | Female reproduction | ECE2013

The combination of genetic variants in the FSHR and FSHB genes affect serum FSH in women of reproductive age

Moriondo Valeria , La Marca Antonio , Papaleo Enrico , Alviggi Carlo , Ruvolo Gianni , Giuseppe De Placido , Candiani Massimo , Cittadini Ettore , De Michele Francesca , Catellani Valeria , Volpe Annibale , Simoni Manuela

Introduction: The relationship between SNPs of the FSHR gene and serum FSH has not been completely clarified. Genetic variants of the FSHB gene have been associated to variation in gene transcription and to serum FSH levels in men. An interesting joint effect of both FSHB −211G>T and FSHR 2039 A>G on male reproductive parameters has been recently observed. No data have been published on the effect of the FSHB −211G>T in combination with the FSHR 2039 A&...

ea0063p644 | Interdisciplinary Endocrinology 1 | ECE2019

Dominance of ovarian follicles is determined by follicle-stimulating hormone receptor (FSHR) and G protein-coupled estrogen receptor (GPER) heteromers

Casarini Livio , Lazzaretti Clara , Paradiso Elia , Riccetti Laura , Sperduti Samantha , Marcozzi Serena , Sayers Niamh , Brigante Giulia , Poti Francesco , La Marca Antonio , Valli Barbara , Falbo Angela , Villani Maria Teresa , Klinger Francesca Gioia , Fane Francesca , Hanyaloglu Aylin Carla , Simoni Manuela

Mechanisms regulating the selection of antral ovarian follicles are poorly understood and supposed to rely on low estrogen levels, decline of follicle-stimulating hormone (FSH) levels and receptor (FSHR) expression on the surface of granulosa cells. These concepts are challenged in-vitro, where apoptosis of human granulosa cells (hGLC) and transfected cell lines is induced by high doses of FSH or FSHR overexpression, while estrogens induce anti-apoptotic signals via n...