Searchable abstracts of presentations at key conferences in endocrinology

ea0032oc5.2 | Reproduction | ECE2013

The (TAAAA)n polymorphism in the SHBG gene is related to prenatal androgenization of female fetus: possible implications of the developmental origin of metabolic disorders

Pamporaki Christina , Xita Nectaria , Lazaros Leandros , Makridimas George , Georgiou Ioannis , kolios George , Plachouras Nikolaos , Tsatsoulis Agathocles

Introduction: The aim of this study was to examine whether the distribution of SHBG (TAAAA)n repeat variants contributes to the exposure of the female fetus to androgen excess, by influencing the, in utero, androgen availability.Methods: The study population consisted of 100 pregnant women that carried female fetuses and underwent the procedure of amniocentesis due to age (older than 35). Blood samples and amniotic fluid samples were dr...

ea0032p512 | Endocrine tumours and neoplasia | ECE2013

Biochemical diagnosis of pheochromocytoma using plasma free normetanephrine, metanephrine and methoxytyramine: importance of supine sampling under fasting conditions

Darr Roland , Peitzsch Mirko , Pamporaki Christina , Prejbisz Aleksander , Fassnacht Martin , Beuschlein Felix , Neumann Hartmut , Januszewicz Andrzej , Lenders Jacques , Eisenhofer Graeme

Objective: To document influences of sampling of blood under supine fasting versus seated non-fasting conditions on diagnosis of pheochromocytoma using plasma concentrations of normetanephrine, metanephrine and methoxytyramine (P-NMN/MN/MTY).Design and methods: P-NMN/MN/MTY were measured by liquid chromatography with tandem mass spectrometry in 695 patients at five centers, two of which complied with requirements for supine sampling after an overnight fa...

ea0099p223 | Adrenal and Cardiovascular Endocrinology | ECE2024

Medication-associated false positive results for plasma normetanephrine, metanephrine and methoxytyramine

Knigge Lara , Potthoff Kristin , Constantinescu Georgiana , Alessi Francesco , Fuld Sybille , Zawadzka Karolina , Bornstein Stefan , Lenders Jacques , Eisenhofer Graeme , Pamporaki Christina

Background: Measurements of plasma metanephrines provide a sensitive test for diagnosis of pheochromocytoma/paraganglioma (PPGL). However, false positive results can pose a diagnostic dilemma for clinicians. The aim of the study was to determine whether commonly prescribed drugs with potential action on the sympathetic nervous system can falsely elevate plasma free normetanephrine, metanephrine and methoxytyramine.Methods: This retrospective study includ...

ea0093oc2 | Oral communication 1: Adrenal Diseases | EYES2023

Morbidity in patients with chronic adrenal insufficiency – cardiovascular risk factors and hospitalization rate compared to population based controls

Chifu Irina , Quinkler Marcus , Altieri Barbara , Hannemann Anke , Volzke Henry , Lang Katharina , Reisch Nicole , Pamporaki Christina , Willenberg Holger S. , Beuschlein Felix , Burger-Stritt Stephanie , Hahner Stefanie

Objective: Patients with adrenal insufficiency (AI) have been found to have increased cardiovascular morbidity, partly associated with nonphysiologic glucocorticoid replacement.Design: We included two separate cohorts (cohort 1 and 2) of patients with chronic primary and secondary AI under standard replacement therapy and compared them to two age- and sex-matched population-based studies (SHIP-TREND/DEGS). Patient cohort 1 comprised 389 individuals asses...

ea0099p14 | Adrenal and Cardiovascular Endocrinology | ECE2024

Improved selectivity of adrenal venous sampling with the use of alternative steroids to cortisol

Alessi Francesco , Costantinescu Georgiana , Peitzsch Mirko , Remde Hanna , Kurzinger Lydia , Schulze Manuel , Lee Myron , Yang Jun , Reinke Martin , Beuschlein Felix , Bornstein Stefan , Lenders Jacques , Pamporaki Christina , Eisenhofer Graeme

Background: Current guidelines recommend adrenal vein sampling (AVS) for the identification of surgically treatable unilateral disease among patients with primary aldosteronism (PA). The cortisol-derived selectivity index (SI) is currently used to assess the success (or selectivity) of adrenal vein catheterization during AVS. However, AVS studies can be non-selective in many cases.Aim: To examine whether the use of SI derived either from 11-deoxycortisol...

ea0099ep252 | Adrenal and Cardiovascular Endocrinology | ECE2024

Biochemical diagnosis of pheochromocytoma/paraganglioma in children and adolescents

Potthoff Kristin , Prodanov Tamara , Knigge Lara , Talvacchio Sara , Hubner Angela , Bornstein Stefan , Lenders Jacques , Peitzsch Mirko , Pacak Karel , Eisenhofer Graeme , Pamporaki Christina

Introduction: Currently, it is unclear whether plasma free or 24-hour urinary metanephrines are preferable for diagnosis of pheochromocytoma/paraganglioma (PPGL) in children.Objectives: To investigate whether measurements of plasma free or 24-hour urinary fractionated metanephrines is a reliable test for screening for PPGL in children.Methods: This retrospective study included data from 60 children with and 78 without PPGL. Data in...

ea0081oc6.1 | Oral Communications 6: Endocrine-Related Cancer | ECE2022

Recurrent disease in patients with sporadic pheochromocytoma and paraganglioma

Li Minghao , Prodanov Tamara , Meuter Leah , Kerstens Michiel , Bechmann Nicole , Prejbisz Aleksander , Fassnacht Martin , Timmers Henri , Beuschlein Felix , Fliedner Stephanie , Robledo Mercedes , Lenders Jacques , Pacak Karel , Eisenhofer Graeme , Pamporaki Christina

Background: It is well established that life-long follow-up is required for patients with hereditary pheochromocytomas and paragangliomas (PPGLs), due to the potential of developing recurrent disease. However, whether follow-up of patients with sporadic PPGLs is necessary, remains unclear.Aims: To examine the prevalence and predictors of recurrent disease in patients with sporadic PPGLs.Materials and method: This multicenter study included retrospective ...

ea0099rc3.5 | Rapid Communications 3: Adrenal and Cardiovascular Endocrinology | Part I | ECE2024

The saline infusion test with mass spectrometric measurements of aldosterone in patients tested for primary aldosteronism

Pamporaki Christina , Remde Hanna , Constantinescu Georgiana , Kuerzinger Lydia , Fuss Carmina Teresa , Peitzsch Mirko , Schulze Manuel , Lee Myron , Yang Jun , Mueller Lisa , Williams Tracy Ann , Reincke Martin , Gruber Sven , Beuschlein Felix , Lenders Jacques , Eisenhofer Graeme

Background: Confirmation of primary aldosteronism (PA) with the saline infusion test (SIT) requires accurate measurements of plasma aldosterone, best achieved by mass spectrometry. Performance of the test and appropriate cut-offs remain inadequately defined.Design and methods: This prospective multicenter cohort study involved 451 patients with suspected PA who underwent a seated SIT. Among these, there were 90 and 76 in whom PA was respectively confirmed and excluded based on...

ea0099p16 | Adrenal and Cardiovascular Endocrinology | ECE2024

False-positive and false-negative results during screening, confirmatory testing and subtyping for suspected primary aldosteronism: lessons from Prosaldo

Constantinescu Andreea Georgiana , Pamporaki Christina , Alessi Francesco , Passauer Jens , Remde Hanna , Kuerzinger Lydia , Fuss CarminaTeresa , Schulze Manuel , Peitzsch Mirko , Horvath Andrea , Yang Jun , Bruedgam Denise , Williams TracyAnn , Reincke Martin , Gruber Sven , Beuschlein Felix , Lenders Jacques , Eisenhofer Graeme

Background: Diagnostic stratification of patients with suspected primary aldosteronism (PA) is a multistep process reliant on tests that are not infallible. Only through prospective studies can diagnostic accuracy be appropriately assessed.Methods: The PROSALDO trial enrolled 819 patients between 2019 and 2023 to assess steroid profiles against routine tests for diagnostic stratification. A combination of these tests and outcome assessments, including me...

ea0081oc13.6 | Oral Communications 13: Adrenal and Cardiovascular Endocrinology 2 | ECE2022

Machine Learning models for the accurate prediction of malignant pheochromocytomas and paragangliomas

Pamporaki Christina , Berends Annika MA , Filippatos Angelos , Prodanov Tamara , Meuter Leah , Prejbisz Aleksander , Beuschlein Felix , Fassnacht Martin , Timmers Henri , Noelting Svenja , Abhyankar Kaushik Ganesh , Contsantinescu Georgiana , Kunath Carola , Wang Katharina , Remde Hanna , Januszewicz Andrzej , Robledo Mercedes , Lenders Jacques , Kerstens Michiel , Pacak Karel , Eisenhofer Graeme

Introduction: Pheochromocytomas and paragangliomas (PPGLs) exhibit an up to 20% malignancy rate. Various clinical, genetic, and pathological features have been proposed as predictors of malignancy. However, until present there are no robust indices to reliably predict metastatic PPGLs.Aim: The aim of the present study was to prospectively validate the value of methoxytyramine as risk marker of metastatic disease and establish a machine learning (ML) mode...