Searchable abstracts of presentations at key conferences in endocrinology

ea0063p637 | Interdisciplinary Endocrinology 1 | ECE2019

Polyphenol health effects on cardiovascular and neurodegenerative disorders: a meta-analysis

Spaggiari Giorgia , Poti Francesco , Zimetti Francesca , Zanotti Ilaria , Santi Daniele

Background: Polyphenols are proposed as protective factors against the occurrence of degenerative diseases affecting several systems, such as cardiovascular and neurocognitive ones. The beneficial effect of polyphenol-enriched diets is assumed to be multifactorial, including direct antioxidant and anti-inflammatory properties, as well as the polyphenol-related capability to modulate lipid metabolism and gut microbiota function. However, the prediction of polyphenolsÂ’ impa...

ea0049ep1112 | Female Reproduction | ECE2017

Biological activity mediated by sfingosine-1-phospate receptors in human primary granulosa cells and immortalized granulosa cell line in vitro

Casarini Livio , Fornari Giulia , Simoni Manuela , Poti Francesco

Introduction: Sphingosine-1-phosphate (S1P) is a sphingolipid mediating proliferative signals in human ovarian follicles, acting through five receptors (S1PR1-5). We characterized the effects of S1PR1 and S1PR3 in vitro, as those mostly expressed in human primary granulosa cells (hGLC) and immortalized, tumor-like granulosa cell line (hGL5).Methods: The S1P-mediated signaling, as well as the activity of S1PR1- and S1PR3-specific agonists (SEW281...

ea0041gp176 | Receptors & Signalling | ECE2016

Luteinizing hormone and human chorionic gonadotropin (hCG) action on the same receptor results in different in vitro intracellular signaling in mouse primary Leydig cells

De Pascali Francesco , Riccetti Laura , Poti Francesco , Tagliavini Simonetta , Trenti Tommaso , Simoni Manuela , Casarini Livio

Luteinizing hormone (LH) and human chorionic gonadotropin (hCG) are two glycoprotein hormones regulating development and reproduction. Despite binding the same receptor (LHCGR), they elicit different intracellular signaling. In vitro non-equivalence of hCG and LH was previously demonstrated in human primary granulosa cells. The aim of this study is to compare the effects of LH and hCG in mouse primary Leydig cells in vitro, naturally expressing the murine rec...

ea0063p645 | Interdisciplinary Endocrinology 1 | ECE2019

Demonstration of follicle-stimulating hormone receptor (FSHR) and G protein-coupled estrogen receptor (GPER) heterodimerization by bioluminescence resonance energy transfer (BRET)

Lazzaretti Clara , Paradiso Elia , Riccetti Laura , Sperduti Samantha , Brigante Giulia , Poti Francesco , Fanelli Francesca , Simoni Manuela , Hanyaloglu Aylin Carla , Casarini Livio

During the menstrual cycle, selection of the dominant follicle is characterized by changes of gonadotropin and steroid hormone receptor expression. We aim to evaluate whether co-existing follicle-stimulating hormone receptor (FSHR) and G protein-coupled estrogen receptor (GPER) form heterodimers, suggesting their role in ovarian follicle selection. FSHR-GPER dimerization was evaluated in transfected HEK293 cells in vitro by bioluminescence resonance energy transfer (B...

ea0063p646 | Interdisciplinary Endocrinology 1 | ECE2019

Spingosine-1-phosphate induces cAMP/PKA-independent pCREB activation, but not steroidogenesis, in human granulosa cells

Paradiso Elia , Lazzaretti Clara , Riccetti Laura , Sperduti Samantha , Antoniani Francesco , Brigante Giulia , Feo Gaetano De , Morini Daria , Iannotti Francesca , Simoni Manuela , Poti Francesco , Casarini Livio

Sphingosine-1 phosphate (S1P) is a lysosphingolipid present in the ovarian follicular fluid at the nanomolar concentration. The physiological functions of S1P are mediated through five specific G protein-coupled receptors (GPCRs), known as S1PR1-5. S1PR1 and S1PR3 are expressed in human primary granulosa lutein cells (hGLC), as well as in the immortalized human primary granulosa cell line hGL5. S1PRs are activated at nanomolar concentrations inducing specific activation or inh...

ea0063p644 | Interdisciplinary Endocrinology 1 | ECE2019

Dominance of ovarian follicles is determined by follicle-stimulating hormone receptor (FSHR) and G protein-coupled estrogen receptor (GPER) heteromers

Casarini Livio , Lazzaretti Clara , Paradiso Elia , Riccetti Laura , Sperduti Samantha , Marcozzi Serena , Sayers Niamh , Brigante Giulia , Poti Francesco , La Marca Antonio , Valli Barbara , Falbo Angela , Villani Maria Teresa , Klinger Francesca Gioia , Fane Francesca , Hanyaloglu Aylin Carla , Simoni Manuela

Mechanisms regulating the selection of antral ovarian follicles are poorly understood and supposed to rely on low estrogen levels, decline of follicle-stimulating hormone (FSH) levels and receptor (FSHR) expression on the surface of granulosa cells. These concepts are challenged in-vitro, where apoptosis of human granulosa cells (hGLC) and transfected cell lines is induced by high doses of FSH or FSHR overexpression, while estrogens induce anti-apoptotic signals via n...