Searchable abstracts of presentations at key conferences in endocrinology

ea0020p565 | Neuroendocrinology, Pituitary and Behaviour | ECE2009

Expression and regulation of HIF-1α and RSUME in murine and human pituitary tumor cells

Shan Bing , Theodoropoulou Marily , Buchfelder Michael , Rachinger Walter , Losa Marco , Stalla Gunter , Arzt Eduardo , Renner Ulrich

Hypoxia inducible factor-1alpha (HIF-1α) is a transcription factor regulating expression of many genes related to oxygen homeostasis in response to hypoxic stress. RSUME is a sumoylation enhancer, which was recently cloned from a pituitary cell line with an increased tumorigenic and angiogenic potential. It has been shown that RSMUE is induced by hypoxia and enhances the sumoylation of HIF-1α. Although many studies demonstrated that the level of HIF-1α is remark...

ea0041ep858 | Pituitary - Basic | ECE2016

Temozolomide has no direct effect on the normal and pathological hormone production in the anterior pituitary and in pituitary tumors

Korthower Daniela , Monteserin-Garcia Jose , Wu Yonghe , Stalla Johanna , Buchfelder Michael , Rachinger Walter , Stalla Gunter K. , Renner Ulrich

The chemotherapeutic agent temozolomide (TMZ) has been applied for the treatment of pituitary carcinomas, atypical pituitary adenomas and prolactinomas resistant to dopamine agonist treatment with considerable success. Both shrinkage of the tumors and a drop of excessive hormone production were observed in TMZ-responsive patients. In order to clarify whether the TMZ-induced suppression of hormone secretion was a consequence of tumor shrinkage or caused by direct effects on hor...

ea0056gp197 | Pituitary Basic | ECE2018

Efficacy of pharmacological USP8 inhibition in human Cushing’s disease tumours in vitro

Theodoropoulou Marily , Perez-Rivas Luis , Albani Adriana , Stalla Gunter , Buchfelder Michael , Flitsch Joerg , Honegger Juergen , Rachinger Walter , Reincke Martin

The tumorigenesis of Cushing’s disease is characterized by somatic mutations in the USP8 gene in almost half of the cases. USP8 encodes for ubiquitin specific protease 8, a deubiquitinase that rescues proteins involved in the regulation of ACTH synthesis in corticotroph cells. In the present study we tested the antisecretory and antiproliferative efficacy of a commercially available specific USP8 inhibitor (IC50 3.1 μM USP8; >90 μM USP7) in immortalized muri...

ea0073oc3.6 | Oral Communications 3: Pituitary and Neuroendocrinology | ECE2021

Expression and possible role of integrins in corticotroph tumours

Chen Xiao , Simon Julia , Luis Perez-Rivas , Jörg Flitsch , Honegger Jürgen , Rachinger Walter , Stalla Günter , Reincke Martin , Theodoropoulou Marily

IntroductionIntegrins are heterodimeric transmembrane proteins composed of alpha and beta subunits that mediate cell-cell and cell-extracellular matrix (ECM) interactions. Several integrins are overexpressed in human cancers and their ECM recognition motif, arginine-glycine-aspartate (RGD), is being utilized for tumour imaging and targeting.AimTo explore the expression and function of RGD-binding integrins in...

ea0073oc8.1 | Oral Communications 8: Pituitary and Neuroendocrinology | ECE2021

Expression and putative role of 14–3-3 proteins in corticotroph tumours

Tang Sicheng , Perez-Rivas Luis Gustavo , Albani Adriana , Rotermund Roman , Flitsch Jörg , Honegger Jürgen , Rachinger Walter , Sigrun Röber , Herms Jochen , Reincke Martin , Theodoropoulou Marily

IntroductionSomatic USP8 mutations are found in around 50% of Cushing’s disease tumours and are located in a single mutational hotspot that contains the recognition site for 14–3-3. These proteins bind to phosphoserine recognition motifs to alter the function and location of their target proteins, and are deregulated in several cancers. AimTo explore the expression and function of 14–3-3 prot...

ea0073oc13.1 | Oral Communications 13: Pituitary and Neuroendocrinology | ECE2021

TP53 mutations in functional corticotroph tumours: prevalence and clinical relevance

Perez-Rivas Luis Gustavo , Simon Julia , Albani Adriana , Rotermund Roman , Hermus Perez-Rivas , Guillaume Assié , Gadelha Monica , Deutschbein Timo , Flitsch Jörg , Honegger Jürgen , Rachinger Walter , Stalla Günter , Reincke Martin , Theodoropoulou Marily

IntroductionTP53 mutations have been rarely reported in pituitary tumours. Recently two exploratory exome sequencing studies have identified somatic TP53 mutations in a small number of functional corticotroph tumours (6/18 and 4/10) with USP8 wild type (wt) status, suggesting that they may be more frequent than previously thought. Nevertheless, the clinical impact of those mutations is still unknown.AimTo det...