Searchable abstracts of presentations at key conferences in endocrinology

ea0081rc1.6 | Rapid Communications 1: Diabetes, Obesity, Metabolism and Nutrition 1 | ECE2022

Evidence for three superimposed components, via which metformin affects blood glucose in obese mice

Kaplanian Mairam , Philippe Cecile , Beghini Marianna , Metz Matthaeus , Abu Eid Sameer , Kautzky-Willer Alexandra , Scherer Thomas , Furnsinn Clemens

Introduction: Despite a plethora of suggested targets and pathways, the mechanism of anti-hyperglycaemic metformin action is still unknown. The present study critically re-analysed protocols broadly applied in preclinical rodent studies.Methods: Obese male C57BL/6J mice on high fat diet were treated with metformin in the form of a single dose, daily intraperitoneal injections, admixture to drinking water, or continuous infusion via intraperitoneal minipu...

ea0081p96 | Diabetes, Obesity, Metabolism and Nutrition | ECE2022

Increased haematocrit mediates lowering of blood glucose in mice exposed to hypoxia or treated with erythropoetin

Scherer Thomas , Metz Matthaeus , Beghini Marianna , Durr Sabine , Corina Luca Andreea , Kaplanian Mairam , Abu Eid Sameer , Kautzky-Willer Alexandra , Furnsinn Clemens

Introduction: Mountain dwellers show a lower prevalence of diabetes, which among countless other factors could be due to lower partial oxygen pressure at high altitude. The present study was to investigate in an experimental setting, if and how reduced oxygen availability affects blood glucose.Methods: Male obese mice on high fat diet were continuously maintained under a normobaric hypoxic atmosphere (10% oxygen) for three months. To exclude indirect eff...

ea0081ep362 | Diabetes, Obesity, Metabolism and Nutrition | ECE2022

Imidazole-osmium reduces elution of lipids from cryofixed rat hepatic tissue for ultrastructural analysis

Durr Sabine , Reipert Siegfried , Krššak Martin , Philippe Cecile , Ehret Viktoria , Luft Matthias , Schintlmeister Arno , Scherer Thomas , Furnsinn Clemens

Introduction: Transmission electron microscopy (TEM) is the main tool for studying the ultrastructural properties of metabolically diseased tissue. For impactful micrographs as well as for interpretation of TEM specimens, the integrity of cellular components is crucial. Elution of substances is a major problem in TEM preparation with no universal solution found so far. Investigating fatty liver disease in a rat model, an important factor is the depiction of undamaged lipid dro...

ea0081p67 | Diabetes, Obesity, Metabolism and Nutrition | ECE2022

Leptin increases VLDL triglyceride secretion and reduces hepatic lipid content in lean male subjects

Metz Matthaeus , Beghini Marianna , Pfleger Lorenz , Wolf Peter , Bastian Magdalena , Harreiter Jurgen , Baumgartner-Parzer Sabina , Marculescu Rodrig , Krebs Michael , Hackl Martina , Trauner Michael , Krssak Martin , Stangl Herbert , Kautzky-Willer Alexandra , Furnsinn Clemens , Scherer Thomas

Background: Leptin reduces hepatic lipid content in lipodystrophic and overweight, relatively hypoleptinemic NAFLD patients. However, the underlying mechanism is unknown. In rodents, the anti-steatotic action of leptin is mediated by an increase in VLDL secretion and depends on an intact vagal innervation of the liver.Methods: In this randomized, placebo-controlled, crossover trial, we study the effects of a single metreleptin injection (0.1 mg/kg body w...

ea0081p352 | Diabetes, Obesity, Metabolism and Nutrition | ECE2022

The effects of an acute metreleptin injection on hepatic lipid metabolism in patients with lipodystrophy

Beghini Marianna , Metz Matthaeus , Wolf Peter , Bastian Magdalena , Hackl Martina , Baumgartner-Parzer Sabina , Kautzky-Willer Alexandra , Trauner Michael , Marculescu Rodrig , Krebs Michael , Harreiter Jurgen , Wabitsch Martin , von Schnurbein Julia , Brandt-Huenemann Stephanie , Stumvoll Michael , Miehle Konstanze , Santini Ferruccio , Ceccarini Giovanni , Magno Silvia , Pelosini Caterina , Krssak Martin , Pfleger Lorenz , Stangl Herbert , Furnsinn Clemens , Scherer Thomas

Objective: Treatment with metreleptin ameliorates hepatic steatosis in patients with lipodystrophy. The anti-steatotic effect of metreleptin is partially independent of its anorexic action, which suggests a direct effect of metreleptin on hepatic lipid metabolism. However, this mechanism is unknown. Based on previous findings in rodents, we hypothesized that metreleptin reduces hepatic lipid content by stimulating very-low density lipoprotein triglyceride (VLDL1-TG) secretion,...