Searchable abstracts of presentations at key conferences in endocrinology

ea0044ec1.2 | Early Career Symposium: Launching your career whatever it may be | SFEBES2016

A career in professions allied to science and medicine

Cabrera-Sharp Victoria

It’s crowded at the top of the academic pyramid. A large percentage of doctoral graduates and research staff will not gain tenure in academic research positions. Universities now provide career advice for graduate students and post-doctoral researchers, usually regarding careers outside of academia, be that in research or otherwise. What, then, for those of us that are still passionate about science and working within academia? A career in science administration may not b...

ea0015p296 | Reproduction | SFEBES2008

Hormonal regulation of 11β-hydroxysteroid dehydrogenase (11βHSD) enzyme activities in boar test is and caput epididymidis

Sharp Victoria , Michael Tony , Fowkes Rob

Cortisol is metabolised by two 11βHSD isoenzymes. Recently, we reported that 11βHSD1 and 11βHSD2 mRNA and protein are co-expressed in boar testis and throughout the male reproductive tract. However, enzyme activities were only detectable in boar testis, caput epididymidis, bulbourethral glands and penile urethra. The objective of the present study was to assess whether cortisol–cortisone metabolism in the boar testis and caput epididymidis could be regulate...

ea0034p328 | Reproduction | SFEBES2014

Hypoxia induces terminal differentiation of primary trophoblast cells in vitro

Cabrera-Sharp Victoria , Read Jordan , Mukherjee Abir , de Mestre Amanda

Equine chorionic gonadotrophin (eCG) is not only essential for the maintenance of early equine pregnancy but is also used therapeutically for synchronisation of farm and laboratory animals. Currently it is extracted from serum of farmed pregnant mares. We have recently reported that under normoxic conditions, BMP4 treatment of equine trophoblast resulted in a dose, and developmental stage-dependent increase in total number of differentiated binucleate cells that secrete eCG. O...

ea0028p291 | Reproduction | SFEBES2012

Activation of the cAMP/GCM1 pathway alters differentiation of equine chorionic gonadotrophin-secreting trophoblast cells

Cabrera-Sharp Victoria , Martyn Abbe , Antczak Douglas , de Mestre Amanda

Chorionic Gonadotrophin (CG) is secreted by both horse and human placenta and is critical to maintenance of early pregnancy. Production of CG is dependent on the differentiation of specialised CG-secreting cells, binucleate (horse) and syncytiotrophoblast (human). We recently showed the transcription factor Glial Cells Missing 1 (GCM1) is rapidly induced in vivo during differentiation of equine binucleate trophoblast. Studies in human syncytiotrophoblast have shown cAMP...

ea0038p373 | Reproduction | SFEBES2015

Identification of novel transcription factors that may regulate transcription of the equine chorionic gonadotrophin beta subunit

Read Jordan , Cabrera-Sharp Victoria , Mirczuk Samantha , Fowkes Robert , de Mestre Amanda

Equine Chorionic Gonadotrophin (eCG) is a heterodimeric glycoprotein produced by terminally differentiated, bi/multi-nucleate trophoblast cells in the placenta of horses and humans. It is responsible for the maintenance of early pregnancy via rescue of the corpus luteum and subsequently promotion of progesterone production. The beta subunit of eCG is expressed at levels ten-fold lower than that of the alpha subunit and confers the glycoproteins specificity to the placenta. Ver...

ea0034p327 | Reproduction | SFEBES2014

Glial cells missing 1 transactivates the equine chorionic gonadotrophin beta promoter

Cabrera-Sharp Victoria , Read Jordan , Kitscha Phoebe , Geddis Amelie , Cartwright Judith , de Mestre Amanda

Placental chorionic gonadotrophin (CG) hormone secretion, critical for maintenance of early pregnancy, is dependent on differentiation of specialised CG-secreting binucleate trophoblast (horse) and syncytiotrophoblast (human). The most abundant genes expressed by binucleate cells are the α and β subunits of equine CG. We recently showed the transcription factor glial cells missing 1 (GCM1) is rapidly induced during differentiation of binucleate trophoblast and mRNA e...

ea0025p271 | Reproduction | SFEBES2011

Increased epididymal Rnase9 expression in Rnase10 (−/−) mice

Sharp Victoria , Krutskikh Anton , Dacheux Jean-Louis , Poutanen Matti , Huhtaniemi Ilpo

Spermatozoa are dependent on the proximal epididymis environment to complete their maturation. However, no single specific factor crucial for this process has been identified. Rnase9 and Rnase10 are expressed specifically in the murine proximal epididymis. Located on chromosome 14C1 only 28 kb apart, they do not show high homology with each other. An orthologue of both genes exist in the rat and human, and ‘Train A’ (porcine orthologue) is the most abun...