Searchable abstracts of presentations at key conferences in endocrinology

ea0032p6 | Adrenal cortex | ECE2013

Activation of EGFR promotes ACC cell proliferation by inducing VEGF autocrine secretion

Gagliano Teresa , Mole Daniela , Gentilin Erica , Bellio Mariaenrica , degli Uberti Ettore , Zatelli Maria Chiara

Adrenocortical cancer (ACC) is a rare and aggressive malignancy. Currently the main therapeutic option is surgery, but due to difficult and delayed diagnosis and to the onset of metastases, medical therapy is often tried. ACC treatment is mainly represented by Mitotane alone or in association with chemotherapy, with variable results. Understanding the molecular mechanisms that regulate ACC proliferation could be useful to identify new therapeutic options. Aim of our study is t...

ea0032p539 | Endocrine tumours and neoplasia | ECE2013

Involvement of PKCβ and PKCδ isoforms in TSH signaling pathway in thyroid cancer cell lines

Mole Daniela , Gentilin Erica , Gagliano Teresa , Tagliati Federico , degli Uberti Ettore , Zatelli Maria Chiara

It is well established that most TSH effects on the thyroid gland, including stimulation of proliferation, thyroid hormone synthesis and expression of thyroid-specific genes, are transmitted mainly by the adenylate cyclise pathway. However, in human follicular cells and in rat FRTL-5 cells, TSH can also stimulate the β-isoforms of PLC that catalyzes the hydrolysis of phosphatidyl-inositol 4,5-phosphate, yielding the second messengers DAG and inositol 1,4,5-phosphate facil...

ea0032p549 | Endocrine tumours and neoplasia | ECE2013

Epidermal growth factor pathway as a possible target in the medical therapy of bronchial carcinoids

Gagliano Teresa , Bellio Mariaenrica , Minoia Mariella , Benfini Katiuscia , degli Uberti Ettore , Zatelli Maria Chiara

Bronchial carcinoids (BC) are rare tumors originating from endocrine cells dispersed in the respiratory epithelium. Currently, the main BC treatment is surgery, that can be curative in most of the cases, but is not feasible for large, infiltrating and metastatic disease. In these settings, medical therapy is often tried, being mainly represented by chemotherapy and radiation in the attempt to reduce tumor mass, while somatostatin analogues are employed for symptomatic control....

ea0020p209 | Endocrine tumours and neoplasia | ECE2009

R171Q MEN1 polymorphism in patients presenting with hyperparathyroidism

Zatelli Maria Chiara , Filieri Carlo , Tagliati Federico , Ambrosio Maria Rosaria , degli Uberti Ettore

The change of aminoacid Arginine (CGG) to Glutamine (CAG) at position 171 (R171Q) in the MEN1 gene has been occasionally reported in MEN1 carriers, but also in 1.4 to 5% subjects among the general population, therefore it is still unclear whether it might represent a polymorphism and/or it has a role in tumourigenesis. The aim of our study was to evaluate the presence of the R171Q polymorphism in patients with MEN1-related states presenting for MEN1 genetic screening. F...

ea0020p592 | Neuroendocrinology, Pituitary and Behaviour | ECE2009

Glucose metabolism alterations in acromegaly

Ambrosio Maria Rosaria , Malaspina Alessandra , De Paola Grazia , Bondanelli Marta , Zatelli Maria Chiara , degli Uberti Ettore

Glucose metabolism alterations are frequently observed in acromegalic patients. Somatostatin analogues (SSTA) are the most widely used drugs to treat acromegaly, since they inhibit GH and IGF-1 levels, reduce pituitary mass, but can affect glucose metabolism. Aim of our study was to evaluate glucose metabolism alterations in acromegalic patients cured after surgery and in patients with active disease during treatment with SSTA. We studied 10 patients (group A, 5F, 55.66±1...

ea0016p316 | Endocrine tumours | ECE2008

Effects of pasireotide on bronchial carcinoids in primary culture

Zatelli Maria Chiara , Tagliati Federico , Luchin Andrea , Bondanelli Marta , Rea Federico , degli Uberti Ettore

Bronchopulmonary endocrine tumors represent 25–30% of lung neoplasms. Surgery provides good survival for differentiated tumors (typical and atypical carcinoids), but is not useful for aggressive poorly differentiated forms. Somatostatin (SRIF) analogs can be used as medical therapy, prolonging patient survival. However, the compounds employed so far did not display antiproliferative effects. Recently, a new stable SRIF analog, pasireotide (SOM230), which activates SSTR1, ...

ea0049ep789 | Endocrine tumours and neoplasia | ECE2017

Magmas modulates chemoresistance in endocrine-related cancers

Riva Eleonora , Tagliati Federico , Gagliano Teresa , Trapella Claudio , Missiroli Sonia , Pinton Paolo , Bertolucci Cristiano , degli Uberti Ettore , Chiara Zatelli Maria

: Magmas, a gene encoding for the mitochondrial import inner membrane translocase subunit, Tim16, protects different cell lines from the antitumoral effects of several pro-apoptotic stimuli (i.e. chemicals and chemotherapeutic agents). The synthetic Compound 5 is capable of sensitizing chemoresistant tumor cells overexpressing Magmas to proapoptotic stimuli, indicating that this molecule may be useful to overcome tumor chemoresistance. The aim of our study was to evaluate whet...

ea0041ep612 | Endocrine tumours and neoplasia | ECE2016

Human endometrial adenocarcinoma: growth hormone involvement in cancer chemoresistance

Gentilin Erica , Di Pasquale Carmelina , Benfini Katiuscia , Falletta Simona , Riva Eleonora , degli Uberti Ettore , Zatelli Maria Chiara

Introduction: It is known that growth hormone (GH) may influence neoplastic development of endometrial epithelium. GH is produced by normal and neoplastic endometrial cells and elevated levels have been observed in endometrial epithelium of patients with endometriosis and endometrial adenocarcinoma (EA). Moreover, endometrium cancer is one of the most occurring tumors in acromegalic patients. Furthermore, autocrine GH increases the oncogenicity of EA cells and is considered fu...

ea0041ep865 | Pituitary - Basic | ECE2016

mTOR pathway: its role in regulating GH secretion in a rat pituitary adenoma cell line

Di Pasquale Carmelina , Gentilin Erica , Bellio Mariaenrica , Benfini Katiuscia , Falletta Simona , Riva Eleonora , degli Uberti Ettore , Zatelli Maria Chiara

Acromegaly results from excess growth hormone (GH) secretion, due to a pituitary adenoma. Surgery is the first option recommended for treatment of GH secreting pituitary adenomas; medical therapy, mostly represented by somatostatin analogues (SSA), is most often used if surgery is not successful. Insulin-like Growth Factor-1 (IGF-1) physiologically reduces GH levels through an endocrine negative feedback loop. IGF-1 exerts its effects also through PI3K/Akt/mTO...

ea0035p11 | Adrenal cortex | ECE2014

VEGF autocrine secretion is enhanced by EGFR activation trough ERK1/2 phosphorylation in human adrenocortical carcinoma cell lines

Gagliano Teresa , Di Pasquale Carmelina , Gentilin Erica , Mole Daniela , Tassinari Martina , Bellio Mariaenrica , degli Uberti Ettore , Zatelli Maria Chiara

Adrenocortical cancer (ACC) is still orphan of medical treatment. Our preliminary data show that EGF induces ACC cell lines proliferation (+20 and +10% vs control in SW13 and NCI-H295 cell lines respectively). EGF receptor (EGFR) expression is higher and ubiquitous in SW13 cells, while it is weaker in NCI-H295 cells, where it is present only on the membrane. Aim of our study is to analyze EGFR downstream signalling in ACC cell lines.EGF induces VEGF synt...