Endocrine Abstracts

We describe a case of a male who presented to a rheumatologist with hypercalceamia at the age of 22 in 1995. Investigations were incomplete and he was lost to follow up. He was referred to a general surgeon in 2002 as another blood test had showed hypercalcaemia of 2.8 mmol/l (2.2–2.6), parathyroid hormone 9.5 pmol/l (1.6–6.9). A spot urine calcium/creatinine excretion ratio was 0.014. It was felt he probably had primary hyperparathyroidism and he was managed conserv...

Context: Preeclampsia is a leading cause of maternal and perinatal morbidity and mortality worldwide, affecting 2–8% of all pregnancies. We have previously shown that women with high-normal levels of FT4 during early pregnancy have a 2.1-fold increased risk of preeclampsia – but there was no apparent association with low TSH. However, the thyroid is stimulated by human chorionic gonadotropin (hCG) during early pregnancy and therefore we hypothesized that women with h...

Objectives: Endothelial cells (ECs) have major role in post-ischemic angiogenesis. EZH2 (enhancer of zest homology) carries out trimethylation of lysine 27 on histone3 (H3K27me3) – repressive mark, thus modulating gene expression. We tested if the EZH2 inhibitor 3-deazaneplanocin (DZNep) regulates angiogenesis under hypoxia and limb ischemia (LI) in vivo in ECs and mouse limb muscles respectively.Methods: Human umbilical vein ECs...

Aims: Hiponatremia is the most frequent electrolyte disturbance. Our aim is to retrospectively analyze the diagnosis of hyponatremia in the discharge sheets of a tertiary hospital during 5 yearsMethod: Data from the MBDS (Minimum Basic Data Set) of the discharged patients from the Hospital Clínico San Carlos (Madrid) between the years 2005 and 2009 was analysed. The 5th Edition of the ICD-9-CM was used for the codification of the diagnoses and proce...

Endothelial cells (ECs) may display distorted epigenetic features in diabetes mellitus (DM) but this has not been investigated under combined conditions of diabetes and limb ischemia. Initial screening for histone H3 and H4 trimethylation (me3) and panacetylation of lysine residues (causing transcriptional repression and activation) were conducted using two models of hyperglycemia and ischemia. Human umbilical vein ECs (HUVECs) were cultured in EGM2 or EBM2 media (low growth f...

It has been hypothesized that primary hyperparathyroidism (PH) has a biphasic disease course. During the first phase hypercalcemia is not yet present. Subsequently, the second phase starts with the development of hypercalcemia. In an attempt to identify differences in the calcium regulating hormonal handling in the clinical setting, we evaluated the hormonal responses of gastrin, PTH, phosphate and ionized calcium to oral peptones (a mixture containing L-amino acids with known...

Background: GH deficiency due to iron overload is well known in thalassaemic children, yet this association has not been described in children with haemochromatosis.Case report and methods: We report on a girl who was first seen at the age of 9.6 years (height 117.5 cm, −3.3 SDS, weight 21 kg, −2.5 SDS). She had a normal physical examination and was prepubertal. Target height-SDS was −0.9. Her bone age was delayed by 10 months. IGF1 was...

Cyclic AMP (cAMP) is the principal intracellular mediator of TSH effects in the thyroid, inducing both thyroid hormone production and cell proliferation. This notion derives from a large series of evidences, mainly obtained by biochemical approaches on cell lines or primary thyrocytes. However, given the limited resolution in time and space of the techniques employed so far, little is know about the spatial localization and temporal dynamics of cAMP signaling in thyroid cells....

Recent studies demonstrate an essential role of the EGF network in propagating the LH signal within ovarian preovulatory follicles. However, the molecular bases for the integration are poorly characterized. Here, we propose that the early LH signal leading to ovulation is amplified through activation of the EGF network.For this study, preovulatory follicles from euthanized gonadotropin-primed mice were isolated and cultured with or without recombinant LH...

Polycystic ovary syndrome (PCOS) is a common endocrine disorder among premenopausal women affecting up to 20% of the population. Even though androgen excess is the main diagnostic criterion for PCOS, the evaluation of hyperandrogenemia relies on methods with low accuracy and mainly able to measure total androgen amounts. Liquid chromatography-tandem mass spectrometry (LC–MS/MS) allows high accuracy steroids measurement and if applied to saliva, it also allows the measurem...