Searchable abstracts of presentations at key conferences in endocrinology

ea0032p388 | Diabetes | ECE2013

Identification of plasma biomarkers in human diabetic retinopathy

Lee Ying-Ray , Lu Chieh-Hsiang , Chan Hong-Lin

Retinopathy has been observed in around 25% of patients with type one and type two diabetes for 3–5 years. Diabetic retinopathy can cause poor vision and even blindness since high glucose has been evidenced to weaken retinal capillary leading to leakage of blood into the surrounding space. Therefore, the prevention, diagnosis and therapy of diabetes retinopathy are very important in diabetes patients. Here, we adopted a proteomics-based approach using 2D-DIGE and MALDI-TO...

ea0031p340 | Steroids | SFEBES2013

Successful use of subcutaneous infusion of cortisol in an adult case of congentital adrenal hyperplasia

Mahgoub Yahya , Kalathil Dhanya , Cuthbert Gary , Hemantha Chan , Purewal Tejpal

Congenital adrenal hyperplasia (CAH) is a group a rare autosomal recessive disorders characterised by a deficiency on one of the enzymes necessary for cortisol biosynthesis. More than 90% of CAH is caused by mutations or deletions in cytochrome P450 21-hydroxylase gene. Impaired glucocorticoid synthesis results in chronic elevation of ACTH causing adrenal hyperplasia and accumulation of steroid precursors such as 17-hydroxyprogesterone (17-HOP). The main goal in CAH managemeng...

ea0029s10.1 | Thyroid: From fetal life to adulthood | ICEECE2012

Placental transport of thyroid hormone

Franklyn J. , Chan S. , Vasilopolou E. , Kilby M.

The transplacental passage of thyroid hormones (TH) from the maternal to fetal circulations is important for normal fetal development, particularly the fetal central nervous system. This is particularly so before the onset of endogenous fetal TH production from the second trimester of pregnancy. The human hemochorial placenta regulates the quantity of TH passing through and the complement of the different forms of TH to ensure requisite levels are present in the fetus for each...

ea0028p311 | Steroids | SFEBES2012

Adrenal expression of the melanocortin-2-receptor and its accessory proteins during sepsis.

Gorrigan Rebecca , Elias Lucila , Clark Adrian , Chan Li

Background: The melanocortin 2 receptor (MC2R)/melanocortin 2 receptor accessory protein (MRAP) are critical components of the hypothalamo-pituitary-adrenal axis (HPA). MRAP2, homologue of MRAP, can also regulate the MC2R in vitro, however, the physiological significance of this is unclear. We studied the expression of the MRAPs and MC2R in a well-defined rat model of endotoxin induced septic shock. These animals have been shown to have higher ACTH and corticosterone le...

ea0025p270 | Reproduction | SFEBES2011

Expression and functional activity of thyroid hormone transporters in microvillous plasma membranes from human term placental syncytiotrophoblasts

Loubiere Laurence , Vasilopoulou Elisavet , Franklyn Jayne , Kilby Mark , Chan Shiao

Background: Thyroid hormones (TH) are vital for fetal and placental development. TH transporters including monocarboxylate transporters 8 and 10 (MCT8, MCT10), organic anion transporters (OATP1A2, OATP4A1) and system-L amino acid transporters (LAT1, LAT2) are expressed in human placenta from 6 weeks of gestation. All of these TH transporters have been localized to the human syncytiotrophoblast layer of placental villi, which is in direct contact with maternal bl...

ea0025p306 | Steroids | SFEBES2011

Why does MRAP2 fail to save familial glucocorticoid deficiency type 2 patients?

Gorrigan Rebecca , Guasti Leonardo , Clark Adrian , Chan Li

Background and aims: The melanocortin-2-receptor accessory protein (MRAP) is essential for melanocortin-2-receptor (MC2R) function through receptor trafficking and signalling, enabling adrenal glucocorticoid synthesis in response to ACTH stimulation. Disabling mutations of MRAP result in life-threatening glucocorticoid deficiency, known as familial glucocorticoid deficiency type 2. MRAP has a single paralogue in the human genome, MRAP2. In vitro MRAP2 has a similar acti...

ea0013s66 | Dealing with MEN | SFEBES2007

Dealing with MEN: A paediatric perspective

Martin Lee , Chan Li , Savage MO , Johnston LB

Multiple endocrine neoplasia type 2 (MEN2) is an autosomal dominant condition that is rare in childhood. MEN2 is sub-classified into three categories that all include familial medullary thyroid carcinoma (MTC). Familial MTC has no other associated tumours. MEN2A is characterised by MTC, phaeochromocytoma and parathyroid tumours and MEN2B consists of MTC, phaeochromocytoma, ganglioneuromatosis and usually a Marfanoid habitus. The identification of RET as the causative gene has ...

ea0007p138 | Growth and development | BES2004

A dominant negative thyroid hormone receptor (TR) beta1 mutant enhances N-Tera-2 (NT2) cell proliferation

Chan S , McCabe C , Franklyn J , Kilby M

The vulnerability of early central nervous system (CNS) development to thyroid hormone (TH) deprivation has been highlighted by studies showing an association between maternal hypothyroxinemia during the first trimester and long-term neurodevelopmental delay in the offspring. The molecular mechanisms underlying this observation are, however, poorly understood. It is known that the actions of triiodothyronine (T3), the active TH metabolite, are primarily mediated by TRs, which ...

ea0005p5 | Bone | BES2003

Determination of a correlation between release of beta c-terminal telopeptides and area of resorption by recombinant rankl-generated human osteoclasts

Chan B , Buckley K , Dutton J , Gallagher J , Fraser W

The discovery of the fundamental involvement of receptor activator for nuclear factor-KB ligand (RANKL) in osteoclast (OC) formation, has enabled the routine generation of human OCs in vitro from human peripheral blood mononuclear cells (PBMCs). Beta C-terminal telopeptide (beta-CTx) is a specific resorption marker for degradation of bone type I collagen by OCs. We have evaluated the correlation between beta-CTx released from dentine wafers on which OC were c...

ea0056gp85 | Diabetes Epidemiology | ECE2018

Association of glucagon-to-insulin ratio and nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus

Mok Ji Oh , Jung Chan Hee , Lee Ki Young

Objective: Although the importance of islet α-cell dysfunction in the pathogenesis of type 2 diabetes has been reappraised, data on whether increase or decrease of glucagon relative to insulin is related with glucose metabolism parameters or metabolic diseases such as nonalcoholic fatty liver disease(NAFLD) in clinical settings are very limited. Therefore, we investigated the association between glucagon-to-insulin ratio(G/I ratio) and presence of NAFLD and metabolic para...