Searchable abstracts of presentations at key conferences in endocrinology

ea0020p12 | Adrenal | ECE2009

Short term regulation of aldosterone secretion after stimulation and suppression experiments in mice

Spyroglou Ariadni , Manolopoulou Jenny , Reincke Martin , Bidlingmaier Martin , Beuschlein Felix

Aldosterone is synthesized acutely upon stimulation of the renin-angiotensin-aldosterone system from the cells of the zona glomerulosa. Several enzymes are involved in this steroidogenic process including the steroidogenic acute regulatory protein (StAR), P450 side chain cleavage enzyme (Cyp11a1) and aldosterone synthase, the product of the gene Cyp11b2.We investigated the short time transcriptional regulation of these genes in wild type mi...

ea0035p203 | Cardiovascular Endocrinology & Lipid Metabolism | ECE2014

ARR screening using IDS-iSYS aldosterone and direct renin: effects of medication in essential and renovascular hypertension, Cushing's syndrome and primary aldosteronism

Sustarsic Riia , Lonati Chiara , Manolopoulou Jenny , Reincke Martin , Bidlingmaier Martin , Morganti Alberto

Background: Identifying hypertensive patients with primary aldosteronism (PA) using the aldosterone to renin ratio (ARR) is crucial for optimizing their treatment. Various medications used to treat hypertension are known to alter levels of aldosterone and renin, thus affecting the ARR. As withdrawing all medication before screening is often unrealistic, we aimed to determine the degree of interference of some commonly used agents on the ARR using assays on the automated IDS-iS...

ea0035p568 | Endocrine tumours and neoplasia | ECE2014

Expression of prolactin receptor in aldosterone-producing adrenal adenomas

Sustarsic Elahu , Riester Anna , Bielohuby Max , Beuschlein Felix , Reincke Martin , Bidlingmaier Martin

Scattered case reports have described patients who have been diagnosed with both primary hyperaldosteronism and prolactinoma. Prolactin receptor (PRLR) is known to be expressed in human adrenal cortex. Furthermore, PRLR mRNA and protein have both been detected in human adrenal tumors. It has also been shown that PRL treatment of adrenal cells is capable of stimulating aldosterone secretion. The contribution of PRL to the development of hyperaldosteronism is u...

ea0020p7 | Adrenal | ECE2009

Use of diurnal rhythm in salivary aldosterone to discriminate between bilateral adrenal hyperplasia and aldosterone producing adenoma

Manolopoulou Jenny , Gerum Sabine , Mulatero Paolo , Spyroglou Ariadni , Reincke Martin , Bidlingmaier Martin

Differential diagnosis between bilateral adrenal hyperplasia (BAH) and aldosterone producing adenoma (APA) in aldosteronism remains challenging in many cases due to the high prevalence of incidentalomas during imaging techniques, the limited sensitivity of orthostatic testing and the technical difficulties of adrenal vein sampling (AVS).We investigated circadian variation in salivary aldosterone (SA) in patients with APA (n=22) and BAH (n=2...

ea0020htb1 | Hot topics: Basic | ECE2009

Hot topics: Basic

Spyroglou Ariadni , Wagner Sibylle , Manolopoulou Jenny , Hantel Constanze , Reincke Martin , Bidlingmaier Martin , Hrabe de Angelis Martin , Beuschlein Felix

Although primary aldosteronism (PA) is considered to be the most prevalent cause of secondary hypertension the underlying genetic mechanisms have been elucidated only for the rare familial forms of the disease. In an attempt to define novel genetic loci involved in the pathophysiology of PA a phenotype-driven mutagenesis screening after treatment with the alkylating agent N-ethyl-N-nitrosourea was established for the parameter aldosterone. The aldosterone values ...

ea0016p28 | Adrenal | ECE2008

Establishment of a mutagenesis screen to identify mice with high aldosterone levels

Spyroglou Ariadni , Wagner Sibylle , Manolopoulou Jenny , Hantel Constanze , Reincke Martin , Bidlingmaier Martin , de Angelis Martin Hrabe , Beuschlein Felix

According to recent studies, primary aldosteronism is considered to be responsible for almost 10% of all cases of arterial hypertension. The genetic background of this common disease, however, has been elucidated only for the rare familial types whereas in the large majority of sporadic cases it still remains unclear. In an attempt to define novel genetic mechanisms of hyperaldosteronism we utilized a random mutagenesis screen after treatment with the alkylating agent N...

ea0063p34 | Adrenal and Neuroendocrine Tumours 1 | ECE2019

Aldosterone measurement in the Diagnosis of primary aldosteronism – comparison between two automated immunoassays and two liquid chromatography tandem mass-spectrometry methods

Fagotto Valentina , Uhl Olaf , Heinrich Daniel , Nirschl Nina , Sturm Lisa , Kratzsch Jurgen , Bae Yoon Ju , Fries Charlotte , Fenske Wiebke , Reincke Martin , Bidlingmaier Martin

Poor agreement between different analytical methods measuring aldosterone concentrations impedes applicability of uniform cut-offs in the diagnostic work-up of primary aldosteronism (PA). We compared 4 analytical methods (2 immunoassays and 2 LC-MS/MS) in plasma samples before and after saline infusion test (SIT). 80 hypertensive patients underwent the standardized diagnostic work-up within the German Conn’s registry. Patients (PA n=39/non-PA n=41) were ...

ea0035s24.1 | Nutrient regulation of metabolism and endocrine systems | ECE2014

Nutrient regulation of metabolism and endocrine systems: using low-carbohydrate/high-fat diets to dissect macronutrient effects on endocrine systems

Bidlingmaier Martin

Low-carbohydrate/high-fat diets (LC–HFD) are a popular way of dieting among the general population as several reports in humans indicate that LC–HFD induce weight loss in overweight subjects without caloric restriction and may improve glucose metabolism. However, regarding this type of diet there is also much controversy in scientific literature because clinical studies and data from animal experiments suggest severe side effects and thus warrant caution. Furthermore...

ea0022s16.3 | Hot topics in pituitary disease | ECE2010

Problems with GH and IGF1 assays: progress?

Bidlingmaier Martin

Measuring circulating concentrations of GH and insulin-like growth factor (IGF1) is crucial to biochemically establish the diagnosis of GH-related disorders such as acromegaly and GH deficiency. Many commercial or in-house immunoassays are in use. Controlled studies and regular external quality assessment schemes document considerable disparities in GH and IGF1 concentrations reported when different assays are used. Among the reasons for the differences are the molecular heter...

ea0016s18.3 | Doping – an issue for clinical endocrinologists | ECE2008

Distinction between endogenous and exogenous GH

Bidlingmaier Martin

Recombinant human growth hormone (rhGH) is abused as a performance enhancing drug, but our knowledge about this is mainly based on rumours, confessions of former athletes and on actions of the police. For many years, the detection of rhGH application by biochemical methods was thought to be impossible. Physiological and biochemical properties of hGH made the development of a test method difficult. rhGH is identical in amino acid sequence and chemical properties to the main, 22...