Searchable abstracts of presentations at key conferences in endocrinology

ea0056gp135 | Female Reproduction | ECE2018

Liquid chromatography-tandem mass spectrometry characterization of a broad serum and salivary steroid profile in classical form of polycystic ovary syndrome

Mezzullo Marco , Fanelli Flaminia , Zanotti Laura , Pagotto Uberto , Gambineri Alessandra

Polycystic ovary syndrome (PCOS) is a common endocrine disorder among premenopausal women affecting up to 20% of the population. Even though androgen excess is the main diagnostic criterion for PCOS, the evaluation of hyperandrogenemia relies on methods with low accuracy and mainly able to measure total androgen amounts. Liquid chromatography-tandem mass spectrometry (LC–MS/MS) allows high accuracy steroids measurement and if applied to saliva, it also allows the measurem...

ea0063gp142 | Interdisciplinary Endocrinology 1 | ECE2019

Impact of age and metabolic profile on the definition of 12 circulating steroid reference intervals measured by LC-MS/MS in an Italian healthy adult male cohort

Mezzullo Marco , Di Dalmazi Guido , Fazzini Alessia , Ibarra Gasparini Daniela , Pelusi Carlotta , Pagotto Uberto , Fanelli Flaminia

Background: Steroid profiling by LC-MS/MS is increasingly used in endocrine research, and is being introduced in the clinical practice. Due to the large disagreement with previous immunoassays, and to the enlarged panel of measurable steroids, novel and robust reference intervals (RIs) need to be established. Steroid circulating levels are known to be influenced by several physio-pathological factors, such as age, gender, stress and metabolic status. Therefore, generating unbi...

ea0063gp143 | Interdisciplinary Endocrinology 1 | ECE2019

Impact of age, menstrual phase, menopausal status and metabolic profile on the definition of 12 circulating steroid reference intervals measured by LC-MS/MS in an Italian healthy adult female cohort

Mezzullo Marco , Di Dalmazi Guido , Fazzini Alessia , Ibarra Gasparini Daniela , Gambineri Alessandra , Pagotto Uberto , Fanelli Flaminia

Endocrine clinical practice strongly relies on normative data for the assessment of hormonal imbalance. However, the generation of robust steroids reference intervals (RIs) remains a challenge. As the steroids circulating levels are influenced by many procedural aspects related to blood collection (time, fasting state) and physio-pathological factors, such as age, sex, stress and metabolic status, the need for partitioning RIs is mandatory. Furthermore, unbiased healthy, drug-...

ea0037gp.05.08 | Developmental and paediatric endocrinology | ECE2015

A simple and rapid method for steroid profiling by two dimensional liquid chromatography–tandem mass spectrometry: toward routine application

Fanelli Flaminia , Mezzullo Marco , Fazzini Alessia , Pedercini Matthew , Pasquali Renato , Pagotto Uberto

Steroid testing has a central role in clinical decision-making and in research studies on diseases such as hypercortisolisms, female hyperandrogenism, male hypogonadism or inborn disorders of steroid synthesis. Recently, liquid chromatography–tandem mass spectrometry (LC–MS/MS) proved its superiority to routine immunoassays (IA) in accurately and sensitively measuring low level steroids. However, the replacement of automated IA with LC–MS/MS platforms is limited...

ea0035p626 | Female reproduction | ECE2014

Simultaneous determination of salivary androgens by liquid chromatography–tandem mass spectrometry: analytical and biological validation

Mezzullo Marco , Fanelli Flaminia , Benetta Elisa Dalla , Garelli Silvia , Gambineri Alessandra , Pagotto Uberto , Pasquali Renato

The hormone assessment in saliva is emerging as a new valuable tool for clinical and research purposes. Salivary levels are supposed to reflect the free fraction of circulating steroids, thus representing a more informative indicator of their activity. Moreover, saliva collection is not stressful and does not require medical assistance, allowing reliable and multiple sampling in a day. Liquid chromatography–tandem mass spectrometry (LC–MS/MS) technology offers high s...

ea0035p757 | Obesity | ECE2014

Circulating endocannabinoids are associated with cardiometabolic impairment independently of adiposity in normal weight subjects

Fanelli Flaminia , Belluomo Ilaria , Cuomo Gaia , Mezzullo Marco , Baccini Margherita , Casadio Elena , Pasquali Renato , Pagotto Uberto

Obesity is characterized by hyperactivated endocannabinoid System (ECS) in brain and periphery and by increased levels of circulating endocannabinoids (cECs), anandamide (AEA) and 2-arachidonoylglycerol (2AG). It is still debated whether in absence of obesity cECs are only modulated by adiposity or if they are associated with cardiometabolic parameters.We recruited 155 healthy normal weight (NW, BMI: 18.5–24.9 kg/m2) drug-free volunteers ...

ea0016p620 | Reproduction | ECE2008

Basal INSL3 levels predict LH and androgen response to GnRH analogue in PCOS women

Patton Laura , Gambineri Alessandra , De Iasio Rosaria , Fanelli Flaminia , Pasquali Renato

In a previous study, we demonstrated that the concept of functional ovarian hyperandrogenism (FOH), as documented by the exaggerated 17OHP response to buserelin (GnRH-analogue) does not represent a specific feature of all women with PCOS, being present in less than half of PCOS and is a condition characterized by more severe hyperandrogenemia, glucose-stimulated B-cell insulin secretion and worse insulin resistance. INSL3 is produced in the Leydig cells and at reduced levels i...

ea0014p214 | (1) | ECE2007

Effects of pharmacological stimulation or blockade of cannabinoid receptor type 1 (CB1) on gene expression in mouse cultured adipocyte cells

Bellocchio Luigi , Cervino Cristina , Fanelli Flaminia , Vicennati Valentina , Pasquali Renato , Pagotto Uberto

The endocannabinoid system has recently emerged as an important modulator of several functions of adipose tissue by altering cell proliferation and gene expression. In this work, we investigated the effects of CB1 activation/blockade in mouse 3T3-L1 adypocite cells by using WIN55,212, a CB1/CB2 agonist and rimonabant, a specific CB1 antagonist, in different experimental settings such as acute treatment on pre-adipocytes and on mature adipocytes, and chronic treatment during di...

ea0056p889 | Adrenal cortex (to include Cushing's) | ECE2018

Are there specific biomarkers able to differentiate non classical congenital adrenal hyperplasia (NCAH) due to 21-hydroxylase deficiency from non-NCAH in a population of naive hyperandrogenic women in the reproductive age?

Oriolo Claudia , Menabo Soara , Baldazzi Lilia , Castelli Silvia , Pagotto Uberto , Mezzullo Marco , Fanelli Flaminia , Gambineri Alessandra

Objective: This study was aimed to evaluate the prevalence of non classical congenital adrenal hyperplasia due to 21-hydroxylase deficiency (21-NCAH) in hyperandrogenic women in the reproductive age attending our Endocrinology Unit and to identify specific phenotypic traits among clinical, biochemical and hormonal features.Setting: Outpatient Unit of Endocrinology, S. Orsola-Malpighi University Hospital of Bologna, Italy.Patients a...

ea0081p273 | Adrenal and Cardiovascular Endocrinology | ECE2022

Salivary profiles of cortisol and cortisone in patients with primary adrenal insufficiency under replacement therapy: a pilot study

Tucci Lorenzo , Vicennati Valentina , Magagnoli Matteo , Colombin Giacomo , Coscia Kimberly , Rotolo Laura , Fanelli Flaminia , Pagotto Uberto , Di Dalmazi Guido

Background: Evaluation of steroid replacement therapy (SRT) in adrenal insufficiency (AI) is challenging for the lack of reliable parameters. Measurement of salivary cortisol and cortisone emerged as a non-invasive tool for AI management, however poorly investigated. Aim: To analyse the cortisol and cortisone circadian rhythm in normal controls (NC) and patients with primary AI (PAI) under different SRTs, and to identify useful biomarkers. <p class="...