Searchable abstracts of presentations at key conferences in endocrinology

ea0077p202 | Metabolism, Obesity and Diabetes | SFEBES2021

Estimation of body fatness in obesity and partial lipodystrophy in relation to eligibility for bariatric surgery: What should be measured?

Efthymiadis Agathoklis , Vasan Senthil K , Tan Garry D , Karpe Fredrik

Introduction: Eligibility for bariatric surgery in the Unighted Kingdom is based on specific body mass index (BMI) thresholds and the presence of obesity-associated complications. Patients with partial lipodystrophy (LD) often have an abundance of metabolic and cardiovascular complications, but their abdominal adiposity does not always substantially increase BMI. The consequence is that patients with LD may be inappropriately excluded from obesity treatments when judged by BMI...

ea0086p76 | Metabolism, Obesity and Diabetes | SFEBES2022

Plasma Erythroferrone is negatively correlated with total body fat

Hilton Catriona , Sabaratnam Rugivan , Neville Matt , Karpe Fredrik

Background: Abdominal fat accumulation is a risk factor for type 2 diabetes and cardiovascular disease, whereas lower body fat is protective. Erythroferrone (ERFE) is a recently discovered protein that increases intestinal iron absorption by inhibiting hepcidin. ERFE has been shown to inhibit BMP2 signalling and work from our group has shown that BMP2 is a differentiation factor for subcutaneous abdominal, but not gluteal, adipocytes. Because of this, we hypothesised that ERFE...

ea0065op4.4 | Bone and Calcium | SFEBES2019

The associations between body fat distribution and bone mineral density in the Oxford Biobank: a cross sectional study

Hilton Catriona , Vasan Senthil , Neville Matt , Karpe Fredrik

Aims: Body composition is associated with bone mineral density, but the precise associations between body fat distribution and bone mineral density (BMD) remain unclear. We hypothesised that the regional adipose tissue depots would have independent associations with BMD.Methods: We used data from 4900 healthy individuals aged 30–50 years old from the Oxford Biobank to analyse associations between regional fat mass and lean mass with total BMD.<p...

ea0038p194 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

Differences between men and women in markers of dietary fatty acid oxidation

McNeil Catriona , Pramfalk Camilla , Pavlides Michael , Karpe Fredrik , Hodson Leanne

Men have a higher prevalence and risk of non-alcoholic fatty liver disease (NAFLD) than age-matched women and this may, in part, be related to differences in fatty acid oxidation. Fasting and postprandial fatty acid oxidation was investigated in 11 healthy men and 11 healthy women matched for age (46±2 years vs 46±2 years (mean±S.E.M.)), BMI (28.0±1 vs 27.0±1 kg/m2) and liver fat content (3.4±0.9 vs 3.9±0.7%) measure...

ea0094oc6.6 | Metabolism, Obesity and Diabetes | SFEBES2023

Low carbohydrate diets in Type 1 diabetes – defining the degree of glycaemic control and nutritional ketosis

Ozoran Hakan , Karpe Fredrik , Dyson Pam , Tan Garry , Matheou Michael

Background: Carbohydrate-restricted diets in type 1 diabetes mellitus (T1DM) are highly controversial. A commonly held concern is that a low carbohydrate diet may more readily result in conversion to diabetic ketoacidosis – one of the most severe complications of poorly managed T1DM. Of note, there is no clear evidence for this phenomenon. We evaluated metabolic profiles in patients with T1DM who had self-selected carbohydrate-restricted diets.<p clas...

ea0094p221 | Metabolism, Obesity and Diabetes | SFEBES2023

Crosstalk between preadipocytes and macrophages modulate depot-specific adipose tissue responses to oestrogen

Cresswell Emily , Pinnick Katherine , Bezbradica Mirkovic Jelena , Karpe Fredrik

Fat distribution influences metabolic risk. Accumulation of fat in the upper body increases metabolic risk, while lower body adipose expansion is protective. Oestrogen promotes lower body adiposity; transgender women on oestrogen therapy show increases in gluteal adipose, while transition to the menopause is associated with a shift in fat distribution towards the upper body, concurrent with changes in metabolic risk. Still, mechanisms governing the depot-specific effects of oe...

ea0077p63 | Metabolism, Obesity and Diabetes | SFEBES2021

Polygenic lipid risk as a precipitant in Type III hyperlipidaemia

Pieri Kyriaki , Trichia Eirini , Neville Matt J , Taylor Hannah , Bennett Derrick , Karpe Fredrik , Koivula Robert W

Background: Type III hyperlipidaemia (T3HL) is characterised by equimolar increases in plasma triglyceride and cholesterol on an APOE2/2 genotype background and conveys a high risk of early-onset cardiovascular disease (CVD). Phenotypic penetrance of T3HL is <10% and precipitated by several endocrine/metabolic disorders such as obesity, diabetes and hypothyroidism. We explored the effect of triglyceride-raising polygenic score precipitating T3HL, in the context of...

ea0081rc10.3 | Rapid Communications 10: Diabetes, Obesity, Metabolism and Nutrition 3 | ECE2022

Primary bile acids differentially regulate differentiation of human abdominal and gluteal pre-adipocytes

Sabaratnam Rugivan , da Conceicao Ismael , Loh Nellie , Christodoulides Constantinos , Karpe Fredrik , Tomlinson Jeremy , Nikolaou Nikolaos

Bile acids (BAs) are synthesised from cholesterol in the liver and promote lipid digestion. An emerging body of evidence, however, suggests that BAs are also key signaling molecules with potent metabolic and endocrine functions, exerting their effects through activation of BA receptors, including the farnesoid-X- (FXR) and the G-protein-coupled- (TGR5) receptors. Disturbed BA synthesis has been associated with type 2 diabetes mellitus and insulin resistance, and recent studies...

ea0065oc1.2 | Metabolism and Obesity | SFEBES2019

Hepatic de novo lipogenesis is suppressed and fat oxidation is increased by omega-3 fatty acids at the expense of glucose metabolism

Green Charlotte , Pramfalk Camilla , Charlton Catriona , Gunn Pippa , Cornfield Thomas , Pavalides Michael , Karpe Fredrik , Hodson Leanne

Background and Aim: Hepatic de novo lipogenesis (DNL) has been implicated in the development of non-alcoholic fatty liver disease (NAFLD). Supplementation with the omega-3 fatty acids (FA) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) decreases intrahepatic triacylglycerol (IHTAG) and plasma TAG concentrations, which is suggested to be mediated through changes in hepatic DNL. We investigated the effects of omega-3 FA supplementation on intrahepatic DNL an...

ea0044p24 | Adrenal and Steroids | SFEBES2016

Tissue-specific regulation of recycling between cortisol and cortisone by insulin and obesity

Anderson Anna , Andrew Ruth , Homer Natalie , Hughes Kate , Karpe Fredrik , Stimson Roland , Walker Brian

Intracellular cortisol is regulated by 11βHSD1. Although the field has focused on regeneration of cortisol from inert cortisone by 11β-reductase activity of 11βHSD1, we have used stable isotope tracers and arteriovenous sampling to quantify simultaneous dehydrogenase (cortisone generation) and reductase (cortisol regeneration) in human adipose and skeletal muscle. In vitro studies suggest insulin regulates this balance of reductase vs dehydrogenase acti...