Searchable abstracts of presentations at key conferences in endocrinology

ea0041gp56 | Cardiovascular endocrinology | ECE2016

The dual FXR/TGR5 agonist INT-767 reduces visceral fat mass, promoting preadipocyte brown differentiation, mitochondrial function and insulin sensitivity in a rabbit model of high fat diet-induced metabolic syndrome

Vignozzi Linda , Cellai Ilaria , Filippi Sandra , Comeglio Paolo , Mello Tommaso , Bani Daniele , Guasti Daniele , Sarchielli Erica , Morelli Annamaria , Maneschi Elena , Vannelli Gabriella Barbara , Adorini Luciano , Maggi Mario

Expanding brown adipose tissue is a potential therapeutic strategy to counteract insulin resistance and metabolic syndrome (MetS). Farnesoid X receptor (FXR) and Takeda G protein-coupled receptor 5 (TGR5) activation enhances insulin sensitivity, suggesting the capacity of FXR/TGR5 agonists to promote brown differentiation in adipose tissue. Treatment with increasing doses of the dual FXR/TGR5 agonist INT-767 (3, 10, 30 mg/Kg bw, daily for 5 days a week, by oral gavage, for 12 ...

ea0081rc10.5 | Rapid Communications 10: Diabetes, Obesity, Metabolism and Nutrition 3 | ECE2022

GLP-1RAs and glucagon can reshape adipose differentiation in vitro by activating the “browning process”

Cantini Giulia , Trabucco Martina , Fei Laura , Propato Arianna , Guasti Daniele , Quartararo Giovanni , Maggi Mario , Mannucci Edoardo , Luconi Michaela

Obesity is associated with increased and dysfunctional white adipose tissue (WAT). Pharmacological approaches of obesity are still far from obtaining a stable weight loss. Glucagon-like peptide-1 receptor agonists (GLP-1RAs) have been proposed as anti-obesity drugs due to their effects on weight loss. Furthermore, dual agonists engaging both GLP-1 and glucagon are currently under investigation for their marked effects on weight loss, although their mechanisms of action is stil...

ea0049gp25 | Adrenal 3 | ECE2017

Tumor microenvironment increases migration/invasion of murine pheochromocytoma SDHB silenced spheroids

D'Antongiovanni Vanessa , Martinelli Serena , Richter Susan , Canu Letizia , Guasti Daniele , Romagnoli Paolo , Pacak Karel , Eisenhofer Graeme , Mannelli Massimo , Rapizzi Elena

Pheochromocytomas (PHEOs) and paragangliomas (PGLs) are rare neuroendocrine tumors. About 30-40% of Pheo/PGLs are due to a germ-line mutation in one of the 13 main susceptibility genes which include the genes encoding the four subunits of the succinate dehydrogenase (SDH - mitochondrial complex II). In PHEO/PGL due to SDHB mutations up to 80% of affected patients develop metastatic disease and no successful cure is at present available. To obtain an experimental model resembli...

ea0070oc1.3 | Adrenal and Cardiovascular Endocrinology | ECE2020

Tumor microenvironment adipose stem cells modulate adrenocortical carcinoma progression

Cantini Giulia , Armignacco Roberta , de Assis Goncalves Diego , Guasti Daniele , Nesi Gabriella , Romagnoli Paolo , Mannelli Massimo , Maggi Mario , Luconi Michaela

The metabolic interplay occurring between the tumor microenvironment and cancer cells may represent a potential target for novel anti-cancer approaches. Among stromal components, adipocytes and adipose precursors have been shown to actively participate in tumor progression in several solid malignancies. In adrenocortical carcinoma (ACC), a rare endocrine neoplasia with a poor prognosis, cancer cells often infiltrate the fat mass surrounding the adrenal organ, enabling a possib...

ea0032p67 | Adrenal Medulla | ECE2013

Succinate dehydrogenase subunit B mutations modify human neuroblastoma cell metabolism and proliferation

Zampetti Benedetta , Rapizzi Elena , Ercolino Tonino , Fucci Rossella , Felici Roberta , Guasti Daniele , Giache Valentino , Bani Daniele , Chiarugi Alberto , Mannelli Massimo

Paragangliomas (PGLs) are rare neuroendocrine tumors. About 30–40% of these tumors are mutated in different susceptibility genes, including those encoding the different subunits of the succinate dehydrogenase, a complex involved both in the tricarboxylic acid cycle and in the oxygen transport chain. The aim of this project was to investigate whether SDHB mutations may account for alterations in cell metabolism and functions. Since PGL cell lines are not available, we used...

ea0041gp136 | Obesity | ECE2016

A novel human fetal brown stem cell functional model to study brown adipogenesis

Franco Alessandra Di , Guasti Daniele , Squecco Roberta , Mazzanti Benedetta , Rossi Francesca , Idrizaj Eglantina , Gallego-Escuredo Jose , Villarroya Francesc , Bani Daniele , Forti Gianni , Vannelli Gabriella Barbara , Luconi Michaela

The potential therapeutic applications of targeting brown adipose tissue open new clinical avenues in fighting against metabolic pathologies. However, due to the limited brown depots in adult humans, dramatically reduced after birth, solid cell models to study human brown adipogenesis and its pathophysiological regulation are urgently needed.In our study, we characterized a novel human model of brown adipose stem cells, hfB-ASC, derived from fetal inters...

ea0035oc11.4 | Diabetes and Obesity 2 | ECE2014

A novel adult human adipose stem cell model from inducible brown fat surrounding pheochromocytoma tumors

Di Franco Alessandra , Guasti Daniele , Mazzanti Benedetta , Ercolino Tonino , Nesi Gabriella , Bani Daniele , Forti Gianni , Mannelli Massimo , Valeri Andrea , Luconi Michaela

The recent discovery of the presence of brown adipose tissue (BAT) also in human adults is pivotal for development of anti-obesity therapies. This type of fat, in fact, mainly consists of brite cells (brown adipocytes appearing for a browning process in white fat) which are involved in adiposity control. The origin of brite cells in adult white adipose tissue (WAT) is still unclear as human cell models are lacking.Our study demonstrated the presence of B...

ea0020htb2 | Hot topics: Basic | ECE2009

Characterization of human adult stem cell populations isolated from subcutaneous and visceral adipose tissue

Baglioni Silvana , Francalanci Michela , Squecco Roberta , Lombardi Adriana , Cantini Giulia , Angeli Roberta , Gelmini Stefania , Guasti Daniele , Benvenuti Susanna , Annunziato Francesco , Bani Daniele , Liotta Francesco , Francini Fabio , Perigli Giuliano , Serio Mario , Luconi Michaela

White adipose tissue acts as an endocrine organ that secretes a variety of adipokines and coordinates a number of biological processes such as energy homeostasis, neuroendocrine and immune functions. Recent studies demonstrated that abundant adipose tissue depots (particularly visceral adipose tissue), by producing inflammatory cytokines, contribute to chronic low-grade inflammation processes which may underlie the pathogenesis of metabolic disorders such as obesity, atheroscl...