Searchable abstracts of presentations at key conferences in endocrinology

ea0081p463 | Thyroid | ECE2022

Management of suspicious neck lymph nodes in patients with differentiated thyroid carcinoma

de Leo Simone , Trevisan Matteo , Colombo Carla , Persani Luca , Vicentini Leonardo , Fugazzola Laura

The management of loco-regional metastases in patients with differentiated thyroid carcinoma is still debated. Current American Thyroid Association guidelines state that in this context the main goal is to differentiate between low-volume metastatic disease that will progress and that remaining stable over time.Aim of our study was to evaluate the behavior of suspicious or cytologically confirmed lymph node metastases, detected after initial treatment (i...

ea0049ep1408 | Thyroid (non-cancer) | ECE2017

Evaluation of disease outcome in patients affected with differentiated thyroid carcinoma treated with total thyroidectomy or lobectomy

Colombo Carla , De Leo Simone , Perrino Michela , Di Stefano Marta , Cantoni Gianmaria , Vicentini Leonardo , Fugazzola Laura

Abstract: An individualized risk-based approach to the treatment of thyroid cancer is being extensively discussed in the recent literature, but controversies remain about the ideal surgical approach. In particular, if some data showed that properly selected thyroid cancer patients can be treated with lobectomy (LT) with excellent clinical outcomes, other results demonstrated an increased incidence of persistent recurrent disease in patients submitted to LT with respect to thos...

ea0041oc14.3 | Thyroid Cancer | ECE2016

Tert and the oxidative stress in papillary thyroid cancer

Muzza Marina , Colombo Carla , Cirello Valentina , Perrino Michela , Vicentini Leonardo , Fugazzola Laura

Abstract: During hormonogenesis, thyroid cells are physiologically exposed to high levels of reactive oxygen species (ROS) which could either be involved in the pathogenesis of thyroid cancer or exert a cytotoxic effect. We analyzed the oxidative status of papillary thyroid cancer (PTC) either directly, by measuring H2O2 generation by NADPH oxidases (NOXs), and indirectly, by evaluating the antioxidant activity of glutathione peroxidase (GPX), which neutr...

ea0016p764 | Thyroid | ECE2008

Clinical features and outcome of papillary thyroid cancers <2 cm: univariate and multivariate analyses

Perrino Michela , Vannucchi Guia , Vicentini Leonardo , Dazzi Davide , Peccoz Paolo Beck

An increasing prevalence of papillary thyroid carcinomas (PTC) of small size have been recorded in recent years. These tumors have a favorable outcome in most cases, and total thyroidectomy without radioiodine therapy is suggested for microcarcinomas (defined as ≤1 cm). The 6th edition of the TNM staging system for thyroid cancers includes in the T1 category tumors ≤2 cm, limited to the thyroid. Aim of the present study was to evaluate the clinical/pathological fea...

ea0014p110 | (1) | ECE2007

Thyroid cancer and pregnancy: clinical outcome and time of diagnosis in a series of 94 women

Michela Perrino , Guia Vannucchi , Leonardo Vicentini , Mannavola Deborah , Beck-Peccoz Paolo , Fugazzola Laura

Thyroid cancer represents the second more frequent tumor among those diagnosed during pregnancy. Indeed, during pregnancy thyroid volume increases by 20–30% and new nodules can appear, due to the effect of choriogonadotropin which stimulates thyroid growth. Hence, it has been proposed that thyroid cancer diagnosed during pregnancy could harbour a poorer prognosis. Aim of the present study was to compare the clinical outcome in the following 3 groups of patients affected w...

ea0063oc1.3 | Calcium and Bone | ECE2019

Menin and EZH2 activities modulate the expression of the long non-coding RNA HAR1B in parathyroid tumors

Morotti Annamaria , Verdelli Chiara , Guarnieri Vito , Muscarella Lucia Anna , Silipigni Rosa , Guerneri Silvana , Vicentini Leonardo , Vaira Valentina , Corbetta Sabrina

Epigenetic deregulation is emerging as a component of the parathyroid tumorigenesis. We identified a long non-coding RNA (LNCRNA) signature clearly distinguishing parathyroid adenomas (PAds) and carcinomas from normal glands. Among the deregulated LncRNAS, HAR1B was upregulated in PAds harboring the common chromosome 11q loss of heterozygosity (Chr11q-LOH; n=10) compared with PAds harboring normal chromosome 11 haplotype (Chr11q-WT; n=12; P&...

ea0049ep1432 | Thyroid (non-cancer) | ECE2017

Expression analyses of HABP2 in neoplastic and normal thyroid tissues: could this gene play a role in the pathogenesis of familial non medullary thyroid cancer?

Muzza Marina , Colombo Carla , Proverbio Maria Carla , Ercoli Giulia , Perrino Michela , Cirello Valentina , Vicentini Leonardo , Ferrero Stefano , Fugazzola Laura

Recently, the G534E variant of the HABP2 gene was reported as the underlying genetic defect in large kindred with non-syndromic familial non medullary thyroid cancer (FNMTC), but these data were not confirmed in additional cohorts. Consistently, we found in our wide series of FNMTC that the HABP2G534E variant is frequent, but does not segregate with the disease. Nevertheless, a possible role for this gene in the pathogenesis of FNMTC cannot definitely ruled out also because co...

ea0037oc8.3 | Endocrine tumours | ECE2015

miR-372 is aberrantly expressed in most parathyroid tumours and might contribute to parathyroid tumourigenesis by inhibiting CDKN1A/p21 and LATS2

Verdelli Chiara , Forno Irene , Vaira Valentina , Guarnieri Vito , Scillitani Alfredo , Cetani Filomena , Vicentini Leonardo , Balza Gianni , Beretta Edoardo , Corbetta Sabrina

We previously described aberrant expression of microRNAs belonging to the chromosome 19 cluster (C19MC) and the close miR-371-373 cluster in two-thirds of parathyroid carcinomas (PCas). Therefore, we investigated the involvement of the miR-371-373 cluster in parathyroid tumours, where miR-372 was the most consistently upregulated. miR-372 is known to be highly expressed in human embryonic stem cells and definitely downregulated upon differentiation. Moreover, miR-372 promotes ...

ea0037gp.30.06 | Endocrine tumours and neoplasia – General | ECE2015

The Wnt/β-catenin pathway regulates the expression of early embryonic stem cell genes in human parathyroid tumours

Verdelli Chiara , Forno Irene , Vaira Valentina , Guarnieri Vito , Scillitani Alfredo , Cetani Filomena , Vicentini Leonardo , Balza Gianni , Beretta Edoardo , Corbetta Sabrina

Though there is no evidence of a constitutive nuclear accumulation of β-catenin, the Wnt/β-catenin pathway might be deregulated in parathyroid tumours. We investigated unphosphorylated active β-catenin distribution by western blot in 16 typical parathyroid adenomas (PAds): β-catenin accumulation in the nuclear protein fractions varied from the levels detected in Caco-2 cells with constitutively active Wnt signalling (three PAds) to the levels measured in HE...

ea0016s20.5 | Translational highlights | ECE2008

V206M polymorphism of the SLC26A6 gene encoding a Cl-oxalate transporter in patients with primary hyperparathyroidism and kidney stones

Corbetta Sabrina , Eller-Vainicher Cristina , Frigerio Marcello , Valaperta Rea , Costa Elena , Vicentini Leonardo , Beck-Peccoz Paolo , Spada Anna

Primary hyperparathyroidism (PHPT) is associated with increased risk of kidney stones. Hypercalciuria and urine oxalate excretion are considered risk factors for urolithiasis in PHPT stone-formers. Recently, the anion-exchanger SLC26A6 has been involved in the oxalate metabolism. Slc26a6-null mice showed hyperoxalemia, hyperoxaluria resulting in dramatic calcium oxalate urolithiasis. We tested the hypothesis that urine oxalate excretion in PHPT patients might be modulat...