Searchable abstracts of presentations at key conferences in endocrinology

ea0014oc7.4 | Reproductive endocrinology I | ECE2007

Capacitation and acrosome reaction in human ejaculated spermatozoa involve activation of a novel SRC tyrosine kinase

Varano Gabriele , Lombardi Adriana , Forti Gianni , Baldi Elisabetta , Luconi Michaela

Tyrosine phosphorylation of proteins is one of the main processes associated with the development of some specific functions of ejaculated human spermatozoa. Although this process, as well as the identity of the phosphorylated targets, has been well characterized, only few tyrosine kinases (TKs) have been identified so far. Moreover, their roles in regulating sperm functions are still unknown.In the present work, we report the presence and localization o...

ea0020p54 | Adrenal | ECE2009

Rosiglitazone interferes with human adrenocortical carcinoma growth in a xenograft mouse model

Mangoni Monica , Gelmini Stefania , Nesi Gabriella , Poli Giada , Cantini Giulia , Lombardi Adriana , Orlando Claudio , Serio Mario , Mannelli Massimo , Luconi Michaela

Adrenocortical carcinoma (ACC) is a rare and aggressive tumour with a poor prognosis, characterized by radio/chemotherapy resistance. The lack of an effective medical treatment is due to the poor knowledge of the mechanisms underlying malignant tumour transformation and aggressiveness. In vitro studies on the ACC H295R cell model have demonstrated that RGZ, an antidiabetic drug belonging to the thiazolidinedione ligands of PPARgamma, blocks cell proliferation/migration ...

ea0014p117 | (1) | ECE2007

A novel activating germline mutation in the RET gene (Y606C) in a patient with medullary thyroid carcinoma

Luconi Michaela , Ercolino Tonino , Lombardi Adriana , Becherini Lucia , Piscitelli Elisabetta , Sole Gaglianò Maria , Serio Mario , Mannelli Massimo

Germline mutations in the RET gene cause MEN2, an inherited cancer syndrome associated with medullary thyroid carcinoma (MTC). We performed genetic analysis on DNA from whole blood of a 58 yr old female affected by a multifocal MTC. Exons 10, 11, 13, 15 and 16 of RET gene were amplified by PCR using specific primers and characterised by direct automatic sequencing. Here, we report a new RET point mutation: a heterozigous missense mutation Y606C, a G to A nucleotide substitutio...

ea0022p722 | Signal transduction | ECE2010

Peroxisome-proliferator-activated receptor gamma is required for modulating endothelial inflammatory response through a novel nongenomic mechanism

Cantini Giulia , Lombardi Adriana , Borgogni Elisa , Francalanci Michela , Ceni Elisabetta , Gelmini Stefania , Poli Giada , Galli Andrea , Serio Mario , Forti Gianni , Luconi Michaela

Besides their well-known anti-diabetic effects, the peroxisome proliferator-activated receptor γ (PPARγ) thiazolidinedione ligands (TZD) have been suggested to also display anti-inflammatory properties. The receptor role in mediating such effects is far from being elucidated. Here, we demonstrate that PPARγ is necessary for TZD to interfere with TNFα and IFNγ inflammatory activity in human endothelial cells. Different PPARγ ligands similarly inhib...

ea0014p13 | (1) | ECE2007

Rosiglitazone interferes with the inflammatory response induced in human endothelial cells by TNFalpha and IFNgamma through a new mechanism involving extracellular signal-regulated kinases (ERKs)

Lombardi Adriana , Piscitelli Elisabetta , Francalanci Michela , Mello Tommaso , Santarlasci Veronica , Gelmini Stefania , Cantini Giulia , Galli Andrea , Cosmi Lorenzo , Annunziato Francesco , Forti Gianni , Serio Mario , Luconi Michaela

Microvascular endothelium is one of the main character and target involved in the inflammatory response. Upon specific activation, endothelial cells massively recruit Th1 IFNgamma secreting lymphocytes at the inflammatory site. In the present study, we investigate the intracellular signalling mediating TNFalpha (T) and IFNgamma (I) inflammatory response in a human endothelial cell line (HMEC-1) in vitro and the interfering effects of rosiglitazone (RGZ), a peroxisome pr...

ea0020htb2 | Hot topics: Basic | ECE2009

Characterization of human adult stem cell populations isolated from subcutaneous and visceral adipose tissue

Baglioni Silvana , Francalanci Michela , Squecco Roberta , Lombardi Adriana , Cantini Giulia , Angeli Roberta , Gelmini Stefania , Guasti Daniele , Benvenuti Susanna , Annunziato Francesco , Bani Daniele , Liotta Francesco , Francini Fabio , Perigli Giuliano , Serio Mario , Luconi Michaela

White adipose tissue acts as an endocrine organ that secretes a variety of adipokines and coordinates a number of biological processes such as energy homeostasis, neuroendocrine and immune functions. Recent studies demonstrated that abundant adipose tissue depots (particularly visceral adipose tissue), by producing inflammatory cytokines, contribute to chronic low-grade inflammation processes which may underlie the pathogenesis of metabolic disorders such as obesity, atheroscl...