Searchable abstracts of presentations at key conferences in endocrinology

ea0088007 | Abstracts | BES2022

The impact of interferon-α on global gene expression in iPSC-derived β- and α-like cells

Sandra Marin-Canas , Florian Szymczak , Maria Ines Alvelos , Stephane Demine , Maikel L Colli , de Beeck Anne Op , Sofia Thomaidou , Lorella Marselli , Arnaud Zaldumbide , Piero Marchetti , Decio L Eizirik

Aim: IFNα is a key regulator of the initial dialogue between pancreatic β-cells and the immune system in type 1 diabetes (T1D). IFNα induces endoplasmic reticulum (ER) stress, insulitis and a massive HLA-ABC overexpression in human β-cells, three histological hallmarks of T1D. Against this background we investigated the global role of IFNα on iPSC-derived islet-like cells, used here to mimic islet cells in the early neonatal period when autoimmunity ag...

ea0057004 | GLP-1 analogs protect beta cells and prevent diabetes in models of Wolfram syndrome | BES2018

GLP-1 analogs protect beta cells and prevent diabetes in models of Wolfram syndrome

Mariana Igoillo-Esteve , Sanna Toivonen , Paraskevi Salpea , Cristina Cosentino , Bahareh Rajaei , Anyishai Musuaya , Nathalie Pachera , Piero Marchetti , Cris Brown , Fumihiko Urano , Eizirik Decio L , Miriam Cnop

Background and aims: Wolfram syndrome is a rare autosomal recessive orphan disease. The clinical manifestations are young onset diabetes, optic nerve atrophy and deafness. Most Wolfram patients carry mutations in WFS1. WFS1 deficiency results in endoplasmic reticulum (ER) stress, leading to neurodegeneration and pancreatic β-cell dysfunction and death. Glucagon-like peptide-1 (GLP-1) analogs and the cAMP inducer forskolin have been shown to protect β-cells f...

ea0065p224 | Metabolism and Obesity | SFEBES2019

Regulation and role of MiR-125b in β-cells

Pizza Grazia , Cheung Rebecca , Rolando Delphine , Nguyen-Tu Marie-Sophie , Cebola Ines , Chabosseau Pauline , Marchetti Piero , Shapiro James , Piemonti Lorenzo , Sakamoto Kei , Smith David M , Rutter Guy A , Martinez-Sanchez Aida

MicroRNAs (miRNAs) are small non-coding RNAs that repress protein production post-transcriptionally. MiRNAs play crucial roles in metabolism, endocrine cells development and in processes altered in T2D, such as insulin secretion. MiR-125b controls proliferation, apoptosis and differentiation of various cell types, although its role in β-cells remains unclear. Recent studies show an association between high levels of circulating miR-125b and hyperglycaemia (HbA1c) in predi...

ea0059p104 | Diabetes & cardiovascular | SFEBES2018

Glucose regulates miR-184 via AMP-activated protein kinase (AMPK) in pancreatic β-cells

Pizza Grazia , Nguyen-Tu Marie-Sophie , Cebola Ines , Yavari Arash , Marchetti Piero , Piemonti Lorenzo , De Koning Eelco , Shapiro AM James , Johnson Paul , Sakamoto Kei , Smith David M , Leclerc Isabelle , Ashrafian Houman , Ferrer Jorge , Martinez-Sanchez Aida

Introduction: Pancreatic β-cells control glucose homeostasis by secreting insulin in response to high glucose. MiRNAs regulate β-cell function and contribute to β-cell failure in type 2 diabetes. MiR-184 regulates β-cell compensatory expansion during pregnancy and obesity and its expression is reduced by glucose through unknown mechanisms. AMPK is a suggested target of antidiabetic drugs and an important energy sensor. Its β-cell-selective inactivation...

ea0094p65 | Metabolism, Obesity and Diabetes | SFEBES2023

Differential DNA methylation during postnatal development establishes a subpopulation of pancreatic beta cells with expression of the insulin secretion-enhancing gene, neuronatin

Yu Vanessa , Yong Fiona , Marta Angellica , Khadayate Sanjay , Bhattach Supriyo , Chen Keran , Georgiadou Eleni , Parveen Nazia , Marselli Lorella , De Luca Carmela , Suleiman Mara , Whilding Chad , Marchetti Piero , Dhawan Sangeeta , Withers Dominic , Rutter Guy , Millership Steven

Subpopulations of beta cells dictate overall ‘pacing’ of insulin secretion across the islet and therefore the control of glycaemia. Functional beta cell subpopulations also exist in human islets and have an altered subtype distribution in type 2 diabetes. However, the molecular control of the establishment and maintenance of beta cell hierarchy is poorly understood. We hypothesised that the epigenome would govern this heterogeneity. We have identified a novel subpopu...

ea0059oc3.1 | Obesity & diabetes | SFEBES2018

Kisspeptin stimulates insulin secretion and modulates serum metabolites in humans

Izzi Engbeaya Chioma , Comninos Alexander N , Clarke Sophie A , Jomard Anne , Yang Lisa , Jones Sophie , Abbara Ali , Narayanaswamy Shakunthala , Chia Eng Pei , Papadopoulou Deborah , Prague Julia K , Bech Paul , Godsland Ian F , Bassett Paul , Sands Caroline , Camuzeaux Stephane , Gomez Romero Maria , Pearce Jake TM , Lewis Matthew R , Holmes Elaine , Nicholson Jeremy , Tan Tricia , Ratnasabapathy Risheka , Hu Ming , Carrat Gaelle , Piemonti Lorenzo , Bugliani Marco , Marchetti Piero , Johnson Paul R , Hughes Stephen J , James Shapiro AM , Rutter Guy A , Dhillo Waljit S

Background: Limited data exists on the hormonal mediators connecting metabolism and reproduction. Animal studies show that the reproductive hormone, kisspeptin, may also be important in metabolism. We investigated the effects of kisspeptin on human metabolism for the first time, to explore possible kisspeptin-mediated links between reproduction and metabolism.Methods: We performed intravenous glucose tolerance tests (IVGTTs) in 15 healthy men (age 25&#17...