Searchable abstracts of presentations at key conferences in endocrinology

ea0081p14 | Adrenal and Cardiovascular Endocrinology | ECE2022

Profound changes of inflammatory and cardiovascular biomarkers in patients with autonomous cortisol secretion and Cushing syndrome

Astrom Ueland Grethe , Methlie Paal , Lovas Kristian , Saevik Ase Bjorvatn , Heie Anette , Sverre Husebye Eystein

Objective: Determine inflammatory and cardiometabolic biomarkers in patients with autonomous cortisol secretion (ACS), compared with healthy controls and patients with overt Cushing syndrome (CS).Method: Serum from prospectively included patients with ACS (n=65), overt CS (n=8), and healthy subjects (n=120) were analysed for 92 different inflammatory biomarkers using proximity extension assay.Results: ACS...

ea0063p426 | Adrenal and Neuroendocrine Tumours 2 | ECE2019

Validation of 24-hour human adrenal steroid rhythms measured by ULTRADIAN interstitial automated microdialysis: Comparison with plasma

Upton Thomas , Methlie Paal , Russell Georgina , Henne Nina , Tsagarakis Stelios , Kampe Olle , Lightman Stafford , Husebye Eystein

Background: Hormones oscillate in circadian and ultradian rhythms. Consequently, single time point measurements are very difficult to interpret. To address this, we developed an automated system of 24-hour ambulatory microdialysis. This allows measurement of free hormone concentrations in subcutaneous interstitial fluid collected while participants continue normal daily activities. To validate the technique for adrenal steroid hormones, we simultaneously sampled interstitial f...

ea0049ep49 | Adrenal cortex (to include Cushing's) | ECE2017

The short Synacthen test revisited – reevaluation of the normal reference range using LCMSMS

Ueland Grethe AEstrom , Methlie Paal , Oksnes Marianne , Lovas Kristian , Thordarson Hrafnkell , Husebye Eystein

Background: The Synachten test is used to diagnose for adrenal insufficiency (AI) and non-classical congenital adrenal hyperplasia (CAH). The cut-off levels for s-cortisol and s-17-hydroxyprogesterone are derived from immunoassays that were not well standardized and are no longer in use. Introduction of liquid chromatography tandem mass spectrometry (LCMSMS) could resolve the lack of standardization of steroid hormone assays and enable increased diagnostic accuracy.<p clas...

ea0044p159 | Neuroendocrinology and pituitary | SFEBES2016

Dynamic hormonal diagnostics of acromegaly and Cushing’s disease

Simunkova Katerina , Russel Georgina , Upton Thomas , Husebye Eystein , Methlie Paal , Lovas Kristian , Lightman Stafford

Pituitary and adrenal gland assessment, is one of the most important aspects of the management of a pituitary and adrenal adenoma.Clinical practice varies widely with regard to assessment of pituitary and adrenal status pre- and post-operative. Pre-operative testing includes dynamic testing to assess function which is not practical in the immediate post-operative period. Instead a single morning serum total hormones are measured while a more definitive a...

ea0041gp24 | Adrenal (2) | ECE2016

Does measurement of serum dexamethasone increase diagnostic accuracy of the overnight dexamethasone-suppression test?

Ueland Grethe AEstrom , Methlie Paal , Thordasron Hrafnkell Baldur , Lovas Kristian , Kelp Oskar , Mellgren Gunnar , Husebye Eystein

Background: 1-mg overnight dexamethasone-suppression test (DST) is commonly used to screen for hypercortisolism. Sensitivity is high (95%), but specificity is low (80%), leading to false positive results. Identifying individuals with abnormal dexamethasone absorption or metabolism could enhance diagnostic accuracy.Aim: Use serum dexamethasone (s-DXT) to increase diagnostic accuracy of DST.Methods: Prospective study of DST for clini...

ea0059oc2.1 | The best of the best | SFEBES2018

24-hour adrenal steroid rhythms are readily detected by ULTRADIAN automated ambulatory microdialysis in man

Upton Thomas , Methlie Paal , Russell Georgina , Tsagarakis Stelios , Kampe Olle , Lightman Stafford , Husebye Eystein

Background: Hormones oscillate in circadian and ultradian rhythms. Single time point samples are difficult to interpret and high frequency measurements are time consuming, expensive and invasive. We developed a minimally invasive technique of ambulatory, automated microdialysis. This allows frequent 24-hour sampling of interstitial fluid while participants continue normal daily activities.Methods: Healthy volunteers (age 18–68, no regular medication...

ea0073aep26 | Adrenal and Cardiovascular Endocrinology | ECE2021

Adrenal steroid profiling in the diagnostics of partial enzyme defects in the adrenals. Establishment of normal cut-off levels using LC-MS/MS

Grethe Åstrøm Ueland , Dahl Sandra R. , Methlie Paal , Husebye Eystein , Thorsby Per Medboe

BackgroundNon-classical congenial adrenal hyperplasia (NCCAH) is an important differential diagnosis in women with acne, hirsutism, menstrual abnormalities and infertility. To diagnose NCCAH can be challenging, and currently used cutoff levels are based on unstandardized immunological assays, no longer in use.ObjectiveDefine LC-MS/MS based cut-off levels for steroid hormones, to improve diagnosis of NCCAH and...

ea0094op1.4 | Neuroendocrinology and Pituitary | SFEBES2023

Ambulatory dynamic assessment in endocrinology: Insights and possibilities

Upton Thomas , Zavala Eder , Methlie Paal , van Faassen Martijn , Lightman Stafford

Rhythms and change are fundamental properties of all living things, having evolved to allow adaptation to life on earth. Rhythmicity can be observed both in behaviour and internal biology of all mammals including humans. Hormonal secretion patterns that occur at circadian and or ultradian frequencies occur commonly in the endocrine system but for practical and pragmatic reasons clinicians tend to sample and attempt to interpret single time point measures. These results can oft...

ea0065p7 | Adrenal and Cardiovascular | SFEBES2019

ULTRADIAN adrenal steroid metabolodynamics successfully discriminates pituitary Cushing’s disease from healthy normal variation

Upton Thomas , Zavala Eder , Fraser Diane , Methlie Paal , Russell Georgina , Tsagarakis Stelios , Kampe Olle , Lightman Stafford , Husebye Eystein

Background: Establishing endocrine diagnoses including pituitary Cushing’s remains challenging with delayed or misdiagnosis being very costly for both patient and the health care system. A key issue is that standard diagnostic tests are typically single time point, single analyte samples, which do not consider dynamic variation intrinsic to endocrine systems. Having previously demonstrated that free tissue hormones correlate strongly with plasma concentrations, we present...

ea0077p139 | Adrenal and Cardiovascular | SFEBES2021

The ULTRADIAN consortium - Ambulatory in vivo micro dialysis in primary adrenal insufficiency, preliminary data

Russell Georgina , Upton Thomas , Zavala Eder , Methlie Paal , Simunkova Katerina , Berinder Katerina , Botusan Ileena , Vassiliadi Dimitria , Tsagarakis Stelios , Kampe Olle , Husebye Eystein , Lightman Stafford , Oksnes Marianne , Bensing Sophie

Patients on long term glucocorticoid replacement therapy have higher rates of morbidity and mortality. One causative factor may be non-physiological cortisol replacement. We have developed as part of the ULTRADIAN consortium ( the U-RHYTHM, an ambulatory bio-sampling device that can collect clinical samples using in-vivo microdialysis every 20 minutes over the 24hour day whilst individuals continue with their normal everyday activities....