Searchable abstracts of presentations at key conferences in endocrinology

ea0031p180 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

A component of transcriptional PRC2 complex, enhancer of zest homology, modulates endothelial cell responses to hypoxia and post-ischemic angiogenesis in a mouse model of limb ischemia

Mitic Tijana , Anannya Orchi , Emanueli Costanza

Endothelial cells (ECs) have major role in post-ischemic angiogenesis. Trimethylation of lysine 27 of histone3 (H3K27me3) is a repressive epigenetic mark carried by EZH2 enzyme, the catalytic part of Polycomb complex2 (PRC2, also comprising the suppressor of zeste 12 homolog (Suz12)). We investigated if in vitro hypoxia and in vivo limb ischemia affect the expression of PRC2 in ECs and mouse limb muscles, respectively. Then, modulation of angiogenic genes by ...

ea0034p231 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2014

EZH2 regulates responses of endothelial cells under hypoxia and during tissue regeneration following limb ischemia

Mitic Tijana , Marchetti Micol , Meloni Marco , Caporali Andrea , Emanueli Costanza

Objectives: Endothelial cells (ECs) have major role in post-ischemic angiogenesis. EZH2 (enhancer of zest homology) carries out trimethylation of lysine 27 on histone3 (H3K27me3) – repressive mark, thus modulating gene expression. We tested if the EZH2 inhibitor 3-deazaneplanocin (DZNep) regulates angiogenesis under hypoxia and limb ischemia (LI) in vivo in ECs and mouse limb muscles respectively.Methods: Human umbilical vein ECs...

ea0028p203 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2012

Epigenetic signature of endothelial cells and muscle tissue in the diabetic models of limb ischemia

Mitic Tijana , Floris Ilaria , Meloni Marco , Madeddu Paolo , Emanueli Costanza

Endothelial cells (ECs) may display distorted epigenetic features in diabetes mellitus (DM) but this has not been investigated under combined conditions of diabetes and limb ischemia. Initial screening for histone H3 and H4 trimethylation (me3) and panacetylation of lysine residues (causing transcriptional repression and activation) were conducted using two models of hyperglycemia and ischemia. Human umbilical vein ECs (HUVECs) were cultured in EGM2 or EBM2 media (low growth f...

ea0086p160 | Adrenal and Cardiovascular | SFEBES2022

Mapping the chromatin-associated-lncRNAs and their enhancer regions between lymphatic and endothelial cells

Mitic Tijana , Chaloner Emma , Zhang Chenyun , Dudnakova Tatiana , Dunn-Davies Hywel

Blood vessels supply nutrients, oxygen and other key molecules necessary for the function of all organs in the body. The endothelial cell (EC) repair and response to injury or hypoxia is also regulated by the interplay of chromatin-modifying enzymes. Likewise, dysregulation of the lymphatic system underlies the development of the metabolic syndrome. New technologies using omics approaches have now allowed the detection of chromatin and RNA molecules that directly interact in n...

ea0013p167 | Diabetes, metabolism and cardiovascular | SFEBES2007

11β-Hydroxysteroid dehydrogenase-1: key regulator in oxysterol metabolism?

Mitic Tijana , McNae Iain , Webster Scott P , Wamil Malgorzata , Walker Brian R , Hadoke Patrick W F , Andrew Ruth

7-Oxysterols modulate lipid transport and promote oxidative stress and apoptosis in the vascular wall. 7-Ketocholesterol (7KC) and 7β-hydroxycholesterol (7βOHC) are inter-converted by 11β-hydroxysteroid dehydrogenase-1 (11βHSD1), better known for metabolising glucocorticoids. Pharmacological inhibition of 11βHSD1 protects against atherosclerosis and these beneficial effects may be mediated through changes in glucocorticoids or oxysterols. To establish ...

ea0025oc1.5 | Young Endocrinologists prize session | SFEBES2011

Atheroprotection by 11β-HSD1 deficiency in ApoE−/− mice: role of both glucocorticoid and 7-oxysterol factors

Mitic Tijana , Hadoke Patrick W F , Chuaiphichai Surawee , Man Taq Y , Miller Eileen , Andrew Ruth , Walker Brian R , Chapman Karen E , Seckl Jonathan R

11β-Hydroxysteroid dehydrogenase type 1 (11β-HSD1) regenerates active glucocorticoids thus amplifying their intracellular actions. 11β-HSD1 deficiency or inhibition, which improve metabolic syndrome and attenuate atherosclerosis in vulnerable rodent strains, is a target for drug development. However, 11β-HSD1 also converts 7-ketocholesterol (7KC) (which accumulates in fatty tissues), to the potentially more atherogenic, 7β-hydroxycholesterol. Whether a...