Searchable abstracts of presentations at key conferences in endocrinology

ea0022p726 | Steroid metabolism & action | ECE2010

Rapid non-genomic effects of cortisol on glucose-induced insulin secretion in humans

Kerlik Jana , Penesova Adela , Vlcek Miroslav , Imrich Richard , Radikova Zofia

For a long time it has been suggested that glucocorticoids (GC) mediate their effects solely on the genomic level. A number of studies reported an existence of rapid GC effects independent on gene expression. These effects may play an important role in stress response or during acute GC therapy. As far as we know, rapid inhibitory GC effects on glucose-induced secretion of insulin have been reported only in animals (1). The aim of our study was to evaluate effects of GC on ear...

ea0070aep54 | Adrenal and Cardiovascular Endocrinology | ECE2020

Sympathoadrenal system function in patients with multiple sclerosis

Vlcek Miroslav , Penesova Adela , Radikova Zofia , Havranova Andrea , Turcani Peter , Kollar Branislav , Sivakova Monika , Imrich Richard

Background: Multiple sclerosis (MS) is a chronic inflammatory autoimmune and neurodegenerative disease of the central nervous system typically affecting young adults. Autonomic dysfunction is commonly detected in patients with multiple sclerosis. Lower response of sympathoadrenal system to various stressors is mainly connected with more developed disease. However, data evaluating sympathoadrenal system function in early MS are limited. Present study investigates stress respons...

ea0029p332 | Cardiovascular Endocrinology and Lipid Metabolism | ICEECE2012

Sympathetic over-activity and low adrenocortical responses to hypoglycemia in patients in early stage of hypertension

Penesova A. , Radikova Z. , Cizmarova E. , Kvetnansky R. , Vlcek M. , Imrich R.

Increased activity of sympathetic nervous system (SNS) was observed in early stage of hypertension onset. The aim of our present study was to estimate catecholamines response to stress stimulus (insulin induced hypoglycaemia) in young males with early diagnosed non-treated hypertension grade 1 (HT) and normotensive controls (NT).Methods: Insulin tolerance test (ITT, 0.1 IU/kg body weight, Actrapid HM, i.v.) was performed in 21 HT male subjects aged 20.0&...

ea0019p311 | Steroids | SFEBES2009

Effects of endogenous and exogenous hypercortisolemia on low-dose ACTH outcome in humans

Kerlik Jana , Penesova Adela , Vlcek Miroslav , Imrich Richard , Starka Luboslav , Radikova Zofia

Context: The interpretation of low-dose ACTH test during endogenous and exogenous hypercortisolemia induced by stress and glucocorticoid therapy in critically ill patients with possible relative adrenal insufficiency.Objective: The hypothesized inhibitory effects of hypercortisolemia on adrenal responsiveness in humans were tested using two model situations in low-dose ACTH testing.Subjects and methods: Nine young healthy women und...

ea0014p65 | (1) | ECE2007

Decreased insulin sensitivity in young lean hypertensive men is not associated with increased visceral fat and changes in plasma adipocytokines

Penesova Adela , Radikova Zofia , Cizmarova Eva , Belan Vitazoslav , Vigas Milan , Koska Juraj

Objective: Increased abdominal visceral adipose tissue (VAT) deposition is associated with insulin resistance in obese and/or hypertensive patients. We investigated the association of insulin sensitivity with the amount of VAT in young, lean, non-treated males with recently established high normal blood pressure or hypertension grade 1 (HT).Subjects and methods: Twenty-one subjects with HT (age 20.3±0.6 years, BMI 22.4±0.5 kg/m2, sys...

ea0014p568 | (1) | ECE2007

The hypothalamic-pituitary-adrenal axis in premenopausal females with psoriatic arthritis

Vlcek Miroslav , Rovensky Jozef , Penesova Adela , Radikova Zofia , Raffayova Helena , Imrich Richard

Objective: Changes of hypothalamic-pituitary-adrenal (HPA) axis activity, in particular decreased production of adrenal androgens, have been observed in patients with rheumatic diseases. However, data on adrenal steroid status in patients with psoriatic arthritis (PsA) are scarce. The present study was aimed at evaluation of HPA status in context of chronic inflammation in glucocorticoid-naïve premenopausal females with PsA.Subjects and methods: Con...

ea0056p571 | Obesity | ECE2018

Effect of 8 weeks of hypocaloric diet and physical activity on thyroid hormones, irisin and insulin sensitivity in obese otherwise healthy subjects.

Penesova Adela , Bajer Boris , Radikova Zofia , Havranova Andrea , Imrich Richard , Vlcek Miroslav

Hypothyroidism is associated with modest weight gain, but there is a lack of clarity regarding subclinical hypothyroidism and obesity. Studies with bariatric surgery showed that 15–25% of extremely obese subjects had subclinical hypothyroidism. Therefore the goal of this study was to evaluate the effect of intensified life style intervention on insulin sensitivity, irisin concentration and thyroid function parameters.Methods: A randomized i...

ea0029p1196 | Obesity | ICEECE2012

Prediabetes in adult GH deficiency is associated with a substantial reduction in serum and adipose tissue expression levels of zinc-α2-glycoprotein

Balaz M. , Ukropcova B. , Kurdiova T. , Penesova A. , Skyba P. , Vlcek M. , Belan V. , Klimes I. , Payer J. , Imrich R. , Smith S. , Tkacova R. , Gasperikova D. , Ukropec J.

Adult GH deficiency (GHD) is associated with prediabetes and low levels of GH are found in obesity. We hypothesized that zinc-α2-glycoprotein (ZAG), a novel adipokine reduced in obesity, is regulated by GH. ZAG serum and adipose tissue expression were measured in three study populations: i) cachectic (BMI=19.3±0.5 kg/m2), lean/overweight (BMI=26.0±0.5 kg/m2) and obese (BMI=36.0±1.6 kg/m2) patients with chronic obstructive pu...

ea0014p22 | (1) | ECE2007

Screening of 120 adipokines in subcutaneous adipose tissue of patients with growth hormone deficiency reveals changed protein levels

Gasperikova Daniela , Ukropec Jozef , Penesova Adela , Sell Henrike , Skopkova Martina , Vlcek Miroslav , Radikova Zofia , Koska Juraj , Belan Vitazoslav , Pura Mikulas , Vanuga Peter , Payer Juraj , Eckel Jurgen , Klimes Iwar

The role of adipokines and inflammatory cytokines of adipose tissue for development of the growth hormone deficiency (GHD)-related metabolic derangements has not yet been completely understood. Therefore, we screened the protein level of 120 adipokines in subcutaneous adipose tissue (ScAT) of patients with GHD in adulthood.Subjects and methods: Sixteen GHD (10M/6F) with BMI 27±1.0 kg/m2, age 30±2 yrs and sixteen controls matched for ...

ea0011p241 | Cytokines and growth factors | ECE2006

Effect of growth hormone deficiency in adults on insulin action and gene expression of selected adipokines in subcutaneous adipose tissue

Penesova A , Skopkova M , Pura M , Vlcek M , Radikova Z , Vorzakova I , Tajtakova M , Vanuga P , Belan V , Koska J , Payer J , Klimes I , Gasperikova D

Growth hormone deficiency (GHD) in adults seems to predispose to insulin resistance (IR). Development of GHD related IR could be associated with adipose tissue (AT) derived signals. To investigate the nature of this pathological regulation we 1) measured gene expressions for leptin, adiponectin, TNFalpha, IL-6, 11beta HSD1, SREBP, PPARgamma in the AT of untreated GHD adults, and 2) assessed effect of euglycemic hyperinsulinemia (HI) at the gene level.Met...