Endocrine Abstracts

The PREDIMED trial showed that long-term adherence to an ad libitum Mediterranean diet (MeDiet) supplemented with extra-virgin olive oil (EVOO) or mixed nuts reduced incident cardiovascular disease (CVD) in older subjects at high risk. No clinical trials have been conducted to demonstrate whether weight loss sustained over time with an energy-restricted healthy diet plus increased physical activity (PA) reduces CVD risk. We designed the PREDIMED-PLUS study, a 6-year, multicent...

Background: Daily injection of progesterone in men is reported to suppress the pituitary LH response to GnRH challenge. We hypothesized that the progesterone nuclear hormone receptor antagonist mifepristone (RU486) would reverse these effects.Study design: Open–label randomised, three period crossover-over study in 12 healthy male subjects. Subjects were treated for 8 days with A/ progesterone 50 mg (IM) alone; B/ progesterone 50 mg + mifepristone 1...

Diabetes is associated with an oxidative stress (OS). Free radicals are responsible of β-cell destruction in type 1 diabetes and their dysfunction in type 2. The purpose of this work is to evaluate preventive effect of antioxidative natural compounds on several models of OS induced on β-cells.The models of oxidative stress were performed with 40 μmol/l H2O2 for 30 min, with a mixture of 0.25 mmol/l hypoxanthine and 10...

Introduction: Plasma glucose concentration at 1h(1hPG) during an oral glucose tolerance test(OGTT) may be a better predictor of future diabetes mellitus(DM) than 2-h post load glucose concentration(2hPG). HIV-infected individuals may have differential risk of DM compared to the general population, and the optimal diagnosis algorithm for DM in HIV-infected persons remain unclear. We evaluated the agreement of the methods 1 hPG vs 2 hPG) for the diagnosis of pre-D...

Hyperaldosteronism and diabetes are two providers of kidney disease, showing molecular and cellular mechanisms in common, such as oxidative stress, causing renal tubular damages. The objective of this study is to evaluate in vitro the protective effect of a natural antioxidant (red wine polyphenols and green tea extract) in the onset of renal dysfunction. Hyperglycemia and hyperaldosteronism are induced respectively by increasing doses of glucose and aldosterone in prim...

Introduction: In vitro monolayer cell cultures are not able to faithfully model the biological features of the three dimensional (3D) structure of solid tumours. There are limited information about the applicability of 3D cell culturing methods for modeling of tumours of endocrine organs.Aim: The aim of our work was the establishment and characterization of three-dimensional in vitro models of various endocrine tumour cells in order to ...

Thyroid paraganglioma (TP) is a rare disorder that sometimes poses problems in differential diagnosis with medullary thyroid carcinoma (MTC). So far, differential diagnosis is solved with the help of some markers that are frequently expressed in MTC (TTF1, calcitonin, and CEA). However, some of these markers are not absolutely specific of MTC and may be expressed in other tumors. Here we report 3 new cases of TP and describe our strategy to design a diagnostic immunohistochemi...

Background: The altered DNA methylation of certain genes in Pituitary Neuroendocrine Tumors (PitNETs) are well known. However little information is available regarding global methylation changes and the process of demethylation in these tumors. In addition, influencing global methylation-demethylation could be a potential new therapeutic option especially in clinically non-functional PitNETs.Material and Methods: Overall, 44 fresh frozen pituitary adenom...