Searchable abstracts of presentations at key conferences in endocrinology

ea0063gp99 | Adrenal and Neuroendocrine - Basic | ECE2019

Implementation of two preclinical ACC models for a comparative drug screen and initial mechanistic investigations

Sharma Ashish , Shapiro Igor , Perge Pal , Igaz Peter , Hantel Constanze

Current systemic treatments are not satisfying for the treatment of adrenocortical carcinoma (ACC). However, translation of preclinically promising approaches were often disappointing indicating that existing tumor models might have inadequately predicted clinical applicability. Thus, our workgroup initiated a comparative drug screen of relevant chemotherapies and therapies targeting IGFR, EGFR, VEGFR/PDGFR and Wnt signalling pathway in the classical NCI-H295R and recently dev...

ea0041gp19 | Adrenal (1) | ECE2016

MUC-1: update on a newly established tumor model for adrenocortical carcinoma

Hantel Constanze , Shapiro Igor , Jung Sara , Reincke Martin , Beuschlein Felix

In an attempt, to overcome the lack of preclinical models for adrenocortical carcinoma (ACC), we recently aimed at the development of patient-individual tumor models for ACC. During these studies one xenograft (MUC-1), derived from a neck metastasis of an ACC, showed extraordinary engraftment properties and sustained tumor growth over several passages in the murine host. During ongoing studies we investigated and compared all currently available xenograft models for ACC (NCI-H...

ea0016oc4.3 | Bone and adrenal | ECE2008

BMP dependent modulation of adrenocortical growth and function

Johnsen Inga K , Spady Lilia , Shapiro Igor , Beuschlein Felix

Bone morphogenic proteins (BMPs) have been demonstrated to impact tumorigenesis in a variety of tumors. As for the adrenal cortex, BMP6 has been implicated as an important modulator of aldosterone secretion. To screen for alterations of BMP dependent pathways in adrenal tumorigenesis we performed gene profiling experiments. By comparing human adrenal carcinoma (ACC) against normal adrenal glands (Co) we detected a down-regulation of various BMPs (e.g. BMP2, BMP5) which was fur...

ea0073pep11.2 | Presented ePosters 11: Adrenal and Cardiovascular Endocrinology | ECE2021

Establishment of novel multidimensional models for the adrenal gland and adrenal tumors

Shapiro Igor , Peitzsch Mirko , Steenblock Charlotte , Bornstein Stefan , Constanze Hantel

The adrenal gland displays an important role in integrating neuronal, immune, vascular, metabolic and endocrine signals under a common organ capsule. The adrenal is the central organ of the stress response system, responsible for responses to acute and chronic stress stimuli and, thereby, playing a major role in numerous stress-related disorders. While for other diseases translational scientists developed programs to generate healthy cells to regenerate damaged tissues in pati...

ea0020p25 | Adrenal | ECE2009

Towards an aldosterone producing cell line from an aldosterone producing adenoma

Lichtenauer Urs , Zwermann Oliver , Shapiro Igor , Mussack Thomas , Reincke Martin , Beuschlein Felix

To date, the in depth analysis of the key molecular mechanisms involved in functional autonomy and tumor formation in aldosterone producing adenomas has been hampered by the rarity of the disease and the lack of adequate tumor cell lines. Herein, we cultivated a primary cell culture of an aldosterone producing adenoma taken from a 40 year old male patient with a left sided adrenal tumor mass. The cells have been passaged 24 times and still continue to grow after nearly 11 mont...

ea0014p112 | (1) | ECE2007

Isolation of the Side population (SP) from murine adrenal glands renders cells with adrenocortical stem cell properties

Lichtenauer Urs , Shapiro Igor , Geiger Klaus , Pulichino Anne-Marie , Drouin Jaques , Beuschlein Felix

Radioactive and transgenic tracing experiments indicate that in the adult adrenal stem cells persist in the periphery of the cortex, which migrate centripetally and populate the inner cortical zones upon differentiation. However, investigation of these cells has been hampered by the lack of known marker genes. Vital Hoechst dye exclusion has been described as a method for isolating a side population (SP) from mouse bone marrow, which was enriched with stem cells. Utilizing thi...

ea0073oc11.2 | Oral Communications 11: Adrenal and Cardiovascular Endocrinoloyg | ECE2021

Extensive preclinical screening of chemotherapeutic agents and molecular targeted Inhibitors reveals potent combinatory treatment for adrenocortical carcinoma (ACC)

Bothou Christina , Sharma Ashish , Shapiro Igor , Adrian Oo , Kim Baek , Beuschlein Felix , Perge Pal , Igaz Peter , Ronchi Cristina L. , Hantel Constanze

Current systemic treatment options for patients with ACCs are far from being satisfactory. DNA damage/repair mechanisms, which involve e.g. ATM/ATR-signalling or RRM1/RRM2 encoded ribonucleotide reductase (RNR) activation commonly contribute to drug resistance. Moreover, also the regulation of RRM2b, the p53-induced alternative to RRM2, is of unclear importance for ACC. Upon extensive drug screening, including a large panel of classical chemotherapies (doxorubicin, etoposide, ...