Searchable abstracts of presentations at key conferences in endocrinology

ea0032p373 | Diabetes | ECE2013

Altered reactivity of pituitary–adrenal axis to stimulation tests and altered tissue metabolism of cortisol in long-standing type 1 Diabetes

Simunkova Katerina , Vondra Karel , Duskova Michaela , Hill Martin , Starka Luboslav

This work aimed to evaluate pituitary–adrenal axis and tissue metabolism of cortisol in patients with long standing type one diabetes.Fifty patients with diabetes type 1 were investigated; age 38±10 years (mean±S.D.), age at diagnosis 25.5±10 years, disease duration 15±8 years, BMI 24.5±2.7 kg/cm2, HbA1c 7.2±1.2%. Control group consisted of 40 healthy volunteers.The study...

ea0044p159 | Neuroendocrinology and pituitary | SFEBES2016

Dynamic hormonal diagnostics of acromegaly and Cushing’s disease

Simunkova Katerina , Russel Georgina , Upton Thomas , Husebye Eystein , Methlie Paal , Lovas Kristian , Lightman Stafford

Pituitary and adrenal gland assessment, is one of the most important aspects of the management of a pituitary and adrenal adenoma.Clinical practice varies widely with regard to assessment of pituitary and adrenal status pre- and post-operative. Pre-operative testing includes dynamic testing to assess function which is not practical in the immediate post-operative period. Instead a single morning serum total hormones are measured while a more definitive a...

ea0037ep41 | Adrenal cortex | ECE2015

Effect of stress-dosed hydrocortisone on physical capacity in patients with Addison's disease

Simunkova Katerina , Jovanovic Nevena , Oksnes Marianne , Lovas Kristian , Husebye Eystein Sverre

The current conventional glucocorticoid (GC) replacement therapy in primary adrenal insufficiency (Addison’s disease) and congenital adrenal hyperplasia (CAH) renders the cortisol levels unphysiological, resulting in very high levels alternating with almost undetectable levels of cortisol over the day. This reduces not merely the patient’s quality of life but increases the patient’s morbidity and mortality. The HPA axis is characterised by a dynamic circadian va...

ea0032p31 | Adrenal cortex | ECE2013

Reliability of serum versus salivary cortisol in ACTH test

Kosak Mikulas , Hana Vaclav , Hill Martin , Simunkova Katerina , Lacinova Zdena , Krsek Michal , Marek Josef

Introduction: Salivary cortisol measurement, representing free cortisol, seems to be a promissing alternative method to serum cortisol. Besides its use in the diagnostics of hypercortisolism, it could be of help in evaluation of adrenocortical reserve. It might be useful especially in patients on estrogen replacement, which modulates transcortin and hence total cortisol levels.Aims: Compare the reliability of salivary vs. serum cortisol assessment during...

ea0026p126 | Female reproduction | ECE2011

Cigarette smoking and progesterone and androgen metabolites in premenopausal women

Duskova Michaela , Simunkova Katerina , Hill Martin , Hruskovicova Hana , Pospisilova Hana , Kralikova Eva , Starka Luboslav

Objective: Chronic smoking alters the circulating levels of sex hormones and possibly also the neuroactive steroids, however, the data available is limited.Patients and methods: Therefore, a broad spectrum of free and conjugated steroids and related substances was quantified by GC–MS and RIA in premenopausal smokers and in age-matched (38.9±7.3 years of age) non-smokers in the follicular (FP) and luteal phases (LP) of menstrual cycle (10 non-sm...

ea0026p127 | Female reproduction | ECE2011

Influence of smoking cessation on steroid spectrum in premenopausal women

Pospisilova Hana , Duskova Michaela , Simunkova Katerina , Hruskovicova Hana , Hill Martin , Kralikova Eva , Starka Luboslav

Background: Smoking represents the most widespread substance dependence in the world. Several studies show the nicotine’s ability to alter women hormonal homeostasis. Women smokers have higher testosterone and lower estradiol levels throughout life compared to women non-smokers. This negatively affects women reproductive function. Furthermore, alteration of neuroactive and neuroprotective steroids occurs in women smokers, which plays an important role in the activity of t...

ea0026p352 | Steroid metabolism | ECE2011

Effect of smoking cessation on steroid balance in postmenopausal women

Hruskovicova Hana , Duskova Michaela , Simunkova Katerina , Hill Martin , Pospisilova Hana , Kralikova Eva , Vondra Karel , Starka Luboslav

Introduction: Cigarette smoking is one of the most serious substance abuse problems. It is generally accepted that nicotine and other chemicals in tobacco smoke alter endocrine function in women, induce changes in female hormonal balance and likely contributes to reported associations of smoking with adverse reproductive outcomes, including menstrual dysfunction, infertility, and earlier menopause. Studies on the effects of smoking and smoking cessation on female sex hormones ...

ea0022p206 | Clinical case reports and clinical practice | ECE2010

The role of salivary α-amylase in prediction of failure of smoking cessation

Duskova Michaela , Simunkova Katerina , Hill Martin , Hruskovicova Hana , Hoskovcova Petra , Kralikova Eva , Starka Luboslav

Background: A high percentage of adult smokers try to quit, but most of these attempts fail. The ability to predict the success or failure of smoking cessation efforts will be useful for clinical practice. Stress response is regulated by two primary neuroendocrine systems. Salivary cortisol has been used as a marker for the hypothalamus-pituitary-adrenocortical axis and salivary α-amylase as a marker for the sympathetic adrenomedullary system.Method...

ea0022p357 | Diabetes | ECE2010

Levels of adrenomedullin during low dose Synacthen test in diabetics type 1

Simunkova Katerina , Bilek Radovan , Hampl Richard , Zamrazil Vaclav , Janickova-Zdarska Denisa , Hill Martin , Vondra Karel

The aim of the study was to obtain the basic data about adrenomedullin after 1 μg ACTH stimulation.Adrenomedullin has direct effect on adrenal cortex secretion, therefore we studied the changes of its concentrations during ACTH stimulation in diabetic patients suspected of hypocorticalism.Thirty-two diabetics type 1 (DM1) were investigated; 16 with and 16 without autoimmune thyroditis (AIT); age 44±10 years (mean±<sm...

ea0022p743 | Steroid metabolism &amp; action | ECE2010

Levels of 7-hydroxy DHEA derivates during menstrual cycle

Hruskovicova Hana , Duskova Michaela , Simunkova Katerina , Hill Martin , Vondra Karel , Starka Luboslav

Introduction: 7-hydroxyl DHEA derivatives are labelled as natural anti-glucocorticoid and neuroprotective substances. Their impact on the production of cytokines, their activation of apoptosis, influence on the migration of macrophages to peripheral tissue, their possible effect on angiogenesis, effect on inflammatory mediators (arachidonic acid metabolites) and their role in the mechanism of oxidative stress have been described.Methods: In our study we ...