Endocrine Abstracts

Objective: Immunoassays of steroids are still used in the diagnosis and biochemical monitoring of patients with congenital adrenal hyperplasia due to 21-hydroxylase deficiency (21 OHD). However, high levels of steroid intermediates can promote cross-reactivity between steroids giving rise to falsely elevated levels.Design: Fifty-nine patients with classic 21OHD (38 women) were studied. Blood samples were collected in the morning after overnight medicatio...

Introduction: Acromegaly is usually not a difficult condition to diagnose if the possibility of this diagnosis has been raised. However, there are a few conditions presenting with some aspects of acromegaly or gigantism but without growth hormone excess. Such cases are described as ‘pseudoacromegaly’ (acromegaloidism).Methods: A female patient was first investigated for GH excess at 10 y for tall stature since infancy (over 97th perc...

Background: Graves’ orbitopathy (GO) is a severe organ-specific autoimmune inflammatory ocular complication most often associated with Graves’ disease (GD). Besides the cosmetic problems these patients develop, GO may also cause severe eye complications threatening the vision of the patient. Additionally, GO complicates the treatment of patients with GD, making the identification of Graves patients at risk for eye disease before they develop symptoms a critical step ...

The prevalence and possible excess risk of thyroid cancer in T1D compared to individuals without T1D is poorly known. Therefore, the aim of this study was to assess excess risk of thyroid cancer in adults with long-term T1D compared to sex- and age-matched control individuals.Study included 4758 individuals with T1D from the Finnish Diabetic Nephropathy (FinnDiane) Study. For each individual, three nondiabetic control individuals who were matched for sex...

Background: Carpal tunnel syndrome (CTS) is common in patients with acromegaly, with a reported prevalence of 19–64%. We have studied CTS in a large national cohort of patients with acromegaly, and the temporal relationship between the diagnosis of the two diseases.Methods: Retrospective nationwide cohort study including patients diagnosed with acromegaly in Sweden between 2005 and 2017. Patients with acromegaly were identified in the Swedish Natio...

BackgroundNon-classical congenial adrenal hyperplasia (NCCAH) is an important differential diagnosis in women with acne, hirsutism, menstrual abnormalities and infertility. To diagnose NCCAH can be challenging, and currently used cutoff levels are based on unstandardized immunological assays, no longer in use.ObjectiveDefine LC-MS/MS based cut-off levels for steroid hormones, to improve diagnosis of NCCAH and...

Objective: The low dose overnight dexamethasone suppression test (1 mg DST) is a sensitive screening test for Cushing’s syndrome. The specificity of the test can be improved by simultaneous analysis of plasma dexamethasone concentrations. A simple and accurate alternative to analysis of plasma cortisol is analysis of salivary cortisone or cortisol following 1 mg DST [1]. We evaluated if analysis of salivary dexamethasone following 1 mg DST could further improve the specif...

Introduction: Glucocorticoids (GCs) play a crucial role in various physiological processes, with adipose tissue (AT) being an important target organ for their metabolic actions. Besides lipolytic action, the mechanistic regulation of GCs in human AT during physiological exposure is still unclear. We integrated transcriptomic and metabolomic data within the AT to identify mechanistic markers of GC action.Methods: In a randomised, cross-over trial, ten pat...

Background: Saliva samples offer the possibility to obtain stress-free non-invasive samples, also for home-testing, especially useful when blood collection is either undesirable or difficult.The aim of this work was to develop an LC–MS/MS method for the determination of clinically relevant steroid hormones cortisol, cortisone, 11-deoxycortisol, 21-deoxycortisol, 17OH-progesterone, aldosterone, corticosterone, deoxycorticosterone, betamethasone, dexamethasone, testosterone...

Background: Indirect methods is developed to estimate reference limits from patient data, i.e. data from a mix of sick and healthy people. The indirect methods use a large number of results. A prerequisite for the methods to work satisfactorily is that the proportion of patients is not too large (typically < 20-30%).Methods: We used direct and indirect methods for estimating reference limits for TSH, FT4, FT3, total T4 and total T3. All analysis were...