Endocrine Abstracts

Increased cortisol production (Cushing’s syndrome) is a rare disease that is sometimes very difficult to diagnose. Cyclical Cushing’s syndrome in particular is a major challenge. In this case of hypercortisolism, which is not always present, longitudinal diagnostic methods are advantageous. In the past, it has already been shown several times that the measurement of cortisol in the scalp hair shows significantly increased values in patients with Cushing’s syndro...

The objective of this project was to develop and characterise a simple immunoassay for the quantification of free 25-hydroxyvitamin D (25OH Vit D). Recent studies suggest that the concentration and genotype of vitamin D binding protein (DBP) are important factors that determine the bioavailability of 25OH Vit D in blood. It has been suggested that measurement of free, non-protein bound 25OH Vit D in serum, may provide more relevant diagnostic information than total 25OH Vit D,...

Background: Vitamin D is an important contributor to musculoskeletal health and its potential involvement has recently been underlined in several non-skeletal diseases. Measurement of circulating levels of 25-hydroxyvitamin D (25OHD) represents the most reliable assessment of vitamin D status. Several assays are available but are not commutable because of a lack of of standardization. The aim of our study was to evaluate the performance of a new ELISA for measurement of 25OHD ...

Recent studies report that nandrolone, a widely used anabolic steroid by athletes to enhance their performance, is produced endogenously in the humans. However, the detection of nandrolone has mainly been indicated by the measurement of its urinary metabolites (19-norandrosterone and 19-noretiocholanone). The aim of this study was to develop a sensitive and specific ELISA for nandrolone to investigate whether the parent steroid can be detected in the urine of healthy volunteer...

It is now generally acknowledged that local tissue concentrations of cortisol and cortisone are modulated by site-specific actions of 11β-hydroxysteroid dehydrogenase (11β-HSD) isoenzymes 1 and 2. Cortisone, the inactive metabolite of cortisol is produced by type 2 11β-HSD. To assess 11β-HSD type 1 and 2 activity, the cortisone/cortisol ratio has to be accurately determined. Immunoassays to measure cortisone levels are not widely available and tend to lack ...

Vasoinhibin, historically also known as 16K PRL, is a peptide hormone with antiangiogenic, antivasodilatatory, and antivasopermeability effects generated by proteolytic cleavage of prolactin (PRL). The discovery of its role in diabetic retinopathy and peripartum cardiomyopathy led to the development of new pharmacological treatments and their evaluation in clinical interventional trials (ClinicalTrials.gov Identifier: NCT03161652 and NCT00998556). Recent insights into the func...

Introduction: Autoantibodies against insulinoma-associated antigen-2 (anti-IA2) are an important marker for diagnosis and prediction of new-onset type 1 diabetes mellitus (T1DM). Here we report on a new prototype Anti-IA2 ELISA with shorter total incubation time (3h15min) compared to the established EUROIMMUN Anti-IA2 ELISA (minimum 17h40min). The study evaluates the performance of the prototype assay.Methods: The clinical performance of the prototype EL...

The measurement of 25OH vitamin D has tremendously evolved since the first competitive protein-binding assay. Amongst the different techniques that are now routinely employed, ELISA still represents a common tool to quantify the level of 25OH Vitamin D in individuals. Several 25OH Vitamin D ELISA assays have been developed and commercialized in the last 3 years. They all differ by the antibody used and by the technology that is applied to release 25OH Vitamin D from its bindin...

Background: Urinary concentrations of the major progesterone metabolite pregnanediol glucuronide (P3G) are used clinically and in research to monitor ovulation. This ELISA is laborious and costly. If it could be replaced by the automated sensitive chemiluminescence assays routinely used for serum this would be of great value.Objective: We aimed to determine the validity of urine progesterone as measured by two widely used automated assays in comparison t...

Background: Urinary concentrations of the major progesterone metabolite pregnanediol glucuronide (P3G) are used clinically and in research to monitor ovulation. This ELISA is laborious and costly. If it could be replaced by the automated sensitive chemiluminescence assays routinely used for serum this would be of great value.Objective: We aimed to determine the validity of urine progesterone as measured by two widely used automated assays in comparison t...