Searchable abstracts of presentations at key conferences in endocrinology

ea0073aep22 | Adrenal and Cardiovascular Endocrinology | ECE2021

Impact of adrenal insufficiency on female sexual function: A preliminary study

Zamponi Virginia , Mazzilli Rossella , Maggio Roberta , Lardo Pina , Olana Soraya , Pugliese Giuseppe , Faggiano Antongiulio , Stigliano Antonio

IntroductionAdrenal insufficiency is a clinical condition that leads to depletion of glucocorticoids, mineralocorticoids and androgens. While in men adrenal androgen deficiency is not clinically significant for testicular testosterone synthesis, in women so far androgen treatment is suggested for the reduction of libido and depression. However, no data is currently available on female sexual function.Primary endpoints<p class="...

ea0081p391 | Endocrine-Related Cancer | ECE2022

Effect of mitotane on male gonadal function

Innocenti Federica , Di Persio Sara , Taggi Marilena , Maggio Roberta , Lardo Pina , Elena Aloini Maria , Canipari Rita , Stigliano Antonio

Mitotane (MTT) currently represents the treatment of choice for adrenocortical carcinoma (ACC). Clinical evidence shows the occurrence of hypogonadism following treatment with this drug, observed more frequently in male patients. The aim of the study, therefore, was to evaluate the impact of MTT treatment on male gonadal function on adult CD1 mice. At the end of the 45 days of treatment, testes were collected for morphological examination, and a blood sample of each animal was...

ea0070ep3 | Adrenal and Cardiovascular Endocrinology | ECE2020

Advantage and trustworthy of cortisol and dexamethasone evaluation in different biological matrices in patients with adrenal masses

Lionetto Luana , Maggio Roberta , Lardo Pina , De Bernardini Donatella , Cipolla Fabiola , Capi Matilde , Simmaco Maurizio , Pugliese Giuseppe , Stigliano Antonio

Biochemical function of adrenal masses is currently based on 1 mg post-overnight dexamethasone suppression test (pDST). Several approaches are recently developed, in order to reduce false positive/negative samples, only in retrospective series. They are based on the correlation of some different parameters, i.e. late-night salivary cortisol (LNSC) vs serum and salivary cortisol pDST; LNSC vs serum and salivary cortisol and serum dexamethasone pDST; LNSC and cortisone vs serum ...

ea0020p623 | Reproduction | ECE2009

Effects of thyroid hormones on ovary granulosa cells: regulation of proliferation, survival and function

Misiti Silvia , Falzacappa Cecilia Verga , Mangialardo Claudia , Bucci Barbara , Raffa Salvatore , Silvestrini Giuliana , Stigliano Antonio , Toscano Vincenzo

It is clinically evident that women suffering from thyroid disorders are associated with frequent occurrence of menstrual disturbances and impaired fertility, and these abnormalities are improved by restoring the euthyroid state. The exact mechanism for such reproductive aberrations is not well known; however, it is conceivable that thyroid hormones might have a direct role in ovarian physiology via receptors in granulosa cells. We evaluated the effect of thyroid hormones (T<s...

ea0016p5 | Adrenal | ECE2008

Modulation of proteomic profile in h295r adrenocortical cell line induced by mitotane

Stigliano Antonio , Cerquetti Lidia , Sampaoli Camilla , Misiti Silvia , Borro Marina , Gentile Giovanna , Argese Nicola , Simmaco Maurizio , Toscano Vincenzo

Background: Mitotane, 1,1-dichloro-2-(o chlorophenyl) -2- (p-chloro phenyl) ethane (o,p′-DDD) is a compound which represents the effective agent in the treatment of the adrenocortical carcinoma (ACC), able to block cortisol synthesis. Nevertheless the biological mechanism induced by this treatment in this cancer remains unknown. Recently, there has been a strong interest in applying proteomics to foster a better understanding of disease proces...

ea0016p691 | Thyroid | ECE2008

The TRβ 1 is essential in mediating T3 action on Akt pathway in human pancreatic insulinoma cells

Misiti Silvia , Falzacappa Cecilia Verga , Patriarca Valentina , Mangialardo Claudia , Michienzi Simona , Moriggi Giulia , Bucci Barbara , Stigliano Antonio , Toscano Vincenzo

Thyroid hormone action, widely recognized on cell proliferation and metabolism, has recently been related to the phosphoinositide 3 kinase (PI3K), an upstream regulator of the Akt kinase and the involvement of the thyroid hormone receptor β1 has been hypothesized. The serine-threonine kinase Akt can regulate various substrates that drive cell mass, proliferation and survival. Its action has also been characterized in pancreatic β-cells. We previously demonstrated tha...

ea0073aep28 | Adrenal and Cardiovascular Endocrinology | ECE2021

Effects of sorafenib, a tyrosin kinase inhibitor, on adrenocortical cancer cell line

Cerquetti Lidia , Bucci Barbara , Raffa Salvatore , Lardo Pina , Maggio Roberta , Simonini Chiara , Petrangeli Elisa , Toscano Vincenzo , Pugliese Giuseppe , Stigliano Antonio

The lack of an effective medical treatment for adrenocortical carcinoma (ACC) has prompted the, search for better treatment protocols for ACC neoplasms. Sorafenib, a tyrosine kinase inhibitor has exhibited effectiveness in the treatment of different human tumors. Therefore, the aim of this study was to understand the mechanism through which sorafenib acts on ACC, especially since treatment with sorafenib alone is sometimes unable to induce a long-lasting antiproliferative effe...

ea0081oc13.5 | Oral Communications 13: Adrenal and Cardiovascular Endocrinology 2 | ECE2022

Machine learning-based texture analysis in the characterization of cortisol secreting vs non secreting adrenocortical incidentalomas in CT scan

Maggio Roberta , Messina Filippo , D'Arrigo Benedetta , Maccagno Giacomo , Lardo Pina , Palmisano Claudia , Biondo Irene , Matarazzo Iolanda , Laghi Andrea , Pugliese Giuseppe , Stigliano Antonio

Adrenal nodular disease is a frequently increasing in the general population with an incidence that reaches almost 10% in the seventh decade of life. More and more evidences show these lesions discovered through diagnostic imaging (CT, MRI) performed for other medical problems (incidentalomas). New radioimaging techniques, exploiting the quantitative variables of imaging, permit to identify an hypothetical pathological tissue. We have applied this potential in a retrospective ...

ea0056p14 | Adrenal cortex (to include Cushing's) | ECE2018

Preoperative treatment with metyrapone in patient with Cushing’s syndrome due to adrenal adenoma: a pilot prospective study

Puglisi Soraya , Perotti Paola , Barbot Mattia , Cosio Paolo , Scaroni Carla , Stigliano Antonio , Lardo Pina , Morelli Valentina , Polledri Elisa , Chiodini Iacopo , Reimondo Giuseppe , Pia Anna , Terzolo Massimo

Background and objectives: Metyrapone has been approved for the treatment of patients with Cushing’s syndrome from all causes, but only few retrospective clinical studies are available. The aim of our study was the prospective assessment of metyrapone as pre-operative treatment.Methods: Before adrenalectomy, 7 patients with ACTH-independent Cushing’s syndrome due to adrenal adenoma were prospectively treated with metyrapone for 3 months, with e...

ea0090p410 | Pituitary and Neuroendocrinology | ECE2023

Impact of Surgery or Medical Treatment with the Selective Glucocorticoid Receptor Modulator Relacorilant on Hypercoagulopathy in Patients with Cushing Syndrome

Simeoli Chiara , Di Paola Nicola , Stigliano Antonio , Lardo Pina , Kearney Tara , Mezosi Emese , Ghigo Ezio , Giordano Roberta , Mariash Cary N. , Donegan Diane , Feelders Richard , Hand Austin , Moraitis Andreas G. , Pivonello Rosario

In patients with Cushing syndrome (CS), hypercoagulability represents a significant concern, leading to elevated risk for thrombotic events. After curative surgery, hypercoagulability persists for several months; CS treatment guidelines recommend anticoagulation therapy for ≤ 3 months. In patients with Cushing disease, hemostatic parameters may even worsen after surgery, independent of surgical outcome; improvements begin ~3 months after successful surgery (Casonato ...