Searchable abstracts of presentations at key conferences in endocrinology

ea0016p25 | Adrenal | ECE2008

Dehydroepiandrosterone (DHEA) exerts a prominent effect on chromaffin PC12 cell differentiation processes

Ziegler Christian G , Langbein Heike , Vukicevic Vladimir , Bornstein Stefan R , Ehrhart-Bornstein Monika , Krug Alexander W

Within the adrenal gland, chromaffin cells and their progenitors are exposed to a wide variety of growth factors and hormones, including adrenal androgens such as DHEA. The DHEA producing-zona reticularis of the adrenal cortex is in close proximity to the neural crest-derived catecholamine-producing chromaffin cells of the medulla, enabling strong paracrine interactions. In vivo studies in humans revealed that congenital adrenal hyperplasia due to 21-hydroxylase ...

ea0016p27 | Adrenal | ECE2008

Modulation of adrenocortical aldosterone and cortisol synthesis by in vitro oxidized low density lipoprotein

Kopprasch Steffi , Ansurudeen Ishrath , Graessler Juergen , Bornstein Stefan R , Pietzsch Jens

Objectives: Oxidative stress is of critical importance in the pathogenesis of endocrinopathies. Since cholesterol serves as a major source of steroid hormone synthesis we investigated the effect of hypochlorite-modified low density lipoprotein (LDL) on aldosterone and cortisol release from human adrenocortical NCI-H295R cells.Methods: Native LDL obtained from healthy volunteers was oxidized to varying degrees by sodium hypochlorite. The resulting modifie...

ea0016p58 | Adrenal | ECE2008

Characterization and differentiation of chromospheres isolated from bovine adrenal medulla

Chung Kuei-Fang , Sicard Flavie , Vukicevic Vladimir , Gebauer Linda , Bornstein Stefan R , Ehrhart-Bornstein Monika

Chromaffin cells from adrenal medulla are the main source of epinephrine and norepinephrine hormones which are known to mediate so called fight-or-flight response to multiple environmental stress conditions. Chromaffin cells together with sympathetic neurons share the same sympathoadrenal cell lineage, but in contrast to neurons they maintain their proliferation during whole life span. However, little is known about chromaffin cells regeneration in an adult adrenal medulla.</p...

ea0016p306 | Endocrine tumours | ECE2008

Distinct catecholamine phenotypes in hereditary pheochromocytoma

Eisenhofer Graeme , Lenders Jacques WM , Mannelli Massimo M , Bornstein Stefan R , Pacak Karel

This study examined whether different forms of hereditary pheochromocytoma are characterized by different catecholamine phenotypes and whether this is reflected by differences in plasma concentrations of normetanephrine, metanephrine and methoxytyramine – the respective O-methylated metabolites of norepinephrine, epinephrine and dopamine. Subjects included 154 patients with hereditary pheochromocytoma, 72 with tumors associated with von Hippel–Lindau (VHL) syndrome, ...

ea0016p563 | Obesity | ECE2008

ERK1/2 MAPKs and Wnt signaling pathways are independently involved in adipocyte-mediated aldosterone secretion

Vleugels Kim , Ehrhart-Bornstein Monika , Bornstein Stefan R , Krug Alexander W

One important risk factor for the development of arterial hypertension is abdominal obesity. Aldosterone, secreted by adrenocortical cells, promotes sodium and water retention and by that regulates blood pressure homeostasis. Increased serum aldosterone levels have been linked to the development of obesity hypertension. Therefore, identifying the link between obesity and increased aldosterone secretion is of high importance for the management of obesity hypertension.<p cla...

ea0081rc14.2 | Rapid Communications 14: Late Breaking | ECE2022

Prevention of glucocorticoid-induced adipose dysfunction and hepatic lipid deposition through cold acclimation in aged mice

Gado Manuel , Ghane Zahra , Heinrich Annett , Wiedersich Denise , Bornstein Stefan R , Rauner Martina , Hofbauer Lorenz C , Henneicke Holger

Glucocorticoids (GC) are one of the most potent anti-inflammatory pharmacological agents. However, owing to their pleiotropic nature, their clinical effectiveness is frequently limited by their deleterious off-target effects. Frequently, metabolic abnormalities arise from GC excess involving the development of dyslipidaemia, insulin resistance and muscle atrophy as well as excessive fat accumulation in both white adipose tissue (WAT) and the liver. In addition, suppressed ther...

ea0081ep55 | Adrenal and Cardiovascular Endocrinology | ECE2022

The asymmetric adrenal in mice and men: Sexual dimorphism and potential hormonal consequences

Bechmann Nicole , Moskopp Mats Leif , Watts Deepika , Steenblock Charlotte , Kanczkowski Waldemar , Wielockx Ben , Bornstein Stefan R , Peitzsch Mirko , Eisenhofer Graeme

Evidence indicates that the adrenals are sexually dimorphic and asymmetric. These differences may have implications for the prevalence and progression of adrenal diseases, which also show asymmetry and sexual dimorphism. The present study aims to characterize the morphological and metabolic differences associated with adrenal sex and asymmetry in mice and humans. Adrenals were compared bilaterally in male and female C57B/6NRj mice with regard to morphological and hormonal char...

ea0073oc12.1 | Oral Communications 12: Diabetes, Obesity, Metabolism and Nutrition | ECE2021

Prevention of glucocorticoid-induced adipose dysfunction through selective activation of β3-adrenergic signaling in mice

Gado Manuel , Heinrich Annett , Wiedersich Denise , Noll Monique , Bornstein Stefan R. , Rauner Martina , Hofbauer Lorenz C. , Henneicke Holger

Despite their therapeutic effectiveness, glucocorticoids’ (GC) clinical usage is frequently limited due to the deleterious metabolic effects. GC-induced obesity arises from excessive fat accumulation in white adipose tissue (WAT) as well as suppressed thermogenic capacity in brown adipose tissue (BAT). Since the sympathetic nervous system (SNS) plays a fundamental role in adipose tissue biology, we examined the interaction between GCs and SNS signaling in the adipose orga...

ea0044p4 | Adrenal and Steroids | SFEBES2016

Generation of human urine-derived steroidogenic cells through lineage conversion: A new technology to study the adrenal gland

Ruiz-Babot Gerard , Hadjidemetriou Irene , Ajodha Sharon Jane , Ghataore Lea , Taylor David , Taylor Norman , Balyura Mariya , Bornstein Stefan R , Guasti Leonardo

Cellular reprogramming describes the process where a fully differentiated, specialized cell type is induced to transform into a different cell. Cell reprogramming techniques can become powerful tools for modelling diseases, drug testing and for personalized cellular therapy. The adrenal cortex is the primary site of steroid synthesis. Adrenal insufficiency, which can be life threatening, is caused by a number of adrenal disorders, and lifelong management of these patients with...

ea0032oc6.5 | Diabetes &amp; Obesity | ECE2013

ESE Young Investigator Award

Chatzigeorgiou Antonios , Chung Kyoung-Jin , Martin Ruben Garcia , Phieler Julia , Grossklaus Sylvia , Bornstein Stefan R , Tzanavari Theodora , Karalis Katia , Chavakis Triantafyllos

Introduction: Macrophages and lymphocytes are considered as major players in adipose tissue (AT) inflammation and liver steatosis, contributing to metabolic dysregulation during obesity. The costimulatory molecules B7.1 and B7.2 are important regulators of T-cell activation and inflammatory reactions, however, their contribution in obesity-related AT and liver low-grade inflammation is poorly characterized.Methods: Mice sufficient or deficient in B7.1 an...