Searchable abstracts of presentations at key conferences in endocrinology

ea0020s4.4 | Gonadal steroid replacement | ECE2009

Diagnosis and treatment of estrogen deficiency in men

Carani Cesare

The discovery of naturally occurring, inactivating mutations of the aromatase gene and of the estrogen receptor (ER) gene in humans shed new light on the precise role of estrogen in several metabolic processes, both in male and female.To date, few clinical cases of males with well-documented congenital aromatase deficiency (11 females and 8 males) have been reported in medical literature. All mutations accounting for aromatase deficiency have been locate...

ea0022p531 | Male reproduction | ECE2010

Prevalence and characterization of hypogonadism among men with human immunodeficiency virus infection: preliminary results

Rochira Vincenzo , Brigante Giulia , Santi Daniele , Zirilli Lucia , Diazzi Chiara , Orlando Gabriella , Carani Cesare , Guaraldi Giovanni

Introduction: Among various comorbidities of human immunodeficiency virus-1 (HIV-1) infection, male hypogonadism is very frequent with a prevalence of 19% in patients treated with highly active anti-retroviral therapy. However, literature data are still lacking and achieved by studies with <300 subjects each.Aim of the study: Prevalence and clinical characterization of hypogonadism among a large number of men with HIV-1.Methods...

ea0022p742 | Steroid metabolism &amp; action | ECE2010

Tissue expression of the CYP19A1 gene and aromatase protein in male cynomolgus monkeys (Macaca fascicularis)

Pignatti Elisa , Lachhab Asmaa , Casarini Livio , Wistuba Joachim , Schlatt Stefan , Carani Cesare , Simoni Manuela

Objective: Aromatase, the key enzyme involved in estrogen synthesis, is expressed in a variety of cells, including peripheral blood leukocytes (PBLs), and tissues. The systematic study of aromatase expression and activity in different tissues in the human and other primates, however, is difficult and still missing. Although is not really known, aromatase expression in PBLs could be used as parameter of aromatase expression/activity making PBLs a potential alternative for other...

ea0022p788 | Thyroid | ECE2010

Role of calcitonin (Ct) in wash-out fluid from fine-needle aspiration (FNAB) in the diagnosis of medullary thyroid cancer (MTC): comparison with basal calcitonin, cytology and pentagastrin stimulation test (Pg-Test)

Rochira Vincenzo , Diazzi Chiara , Zirilli Lucia , Romano Stefania , Carani Cesare , Madeo Bruno

Introduction: Serum Ct is considered the most sensitive tool for MTC screening, but with poor specificity. Pg-test sensitivity and specificity are high, but doubtful for 30–100 pg/ml of Ct peak. FNAB is not routinely used due to its very low sensitivity and specificity. Only few studies from a small number of patients are available on the role of Ct measurement in the wash-out fluid (Ct-FNAB) for MTC diagnosis, providing conflicting results.Aim of t...

ea0020p483 | Obesity and Metabolism | ECE2009

Aromatase expression in peripheral blood leukocytes from adult and elderly female and male subjects

Pignatti Elisa , Rossi Alessandra , Scaltriti Sara , Taliani Erica , Rochira Vincenzo , Simoni Manuela , Carani Cesare

Objective: Aromatase, the key enzyme involved in estrogen synthesis, is expressed in a variety of cells and tissues including human peripheral blood leukocytes (PBLs). The present study was designed to evaluate PBL aromatase gene expression in male and female elderly subjects compared to young male controls.Design: CYP19A1 mRNA and protein were measured in PBLs obtained from young men (n=13) aged < 35 years, postmenopausal women (n</...

ea0020p596 | Neuroendocrinology, Pituitary and Behaviour | ECE2009

GH deficiency in HIV-infected patients with lipodystrophy: preliminary data on the effects of r-hGH treatment on body composition

Zirilli Lucia , Orlando Gabriella , Brigante Giulia , Squillace Nicola , Diazzi Chiara , Carani Cesare , Guaraldi Giovanni , Rochira Vincenzo

Introduction: HIV-infected patients often display a moderate to severe GH deficiency (GHD). To investigate the effects of r-hGH on hormonal parameters and body composition in patients with HIV-related lipodystrophy (HIV-r-L) and concomitant GHD, we studied 60 patients with HIV-r-L, 28 male and 32 females aged 18–65 years. According to their response to GHRH+Arginine (GH peak assumed to be normal when >7.5 ng/ml) patients were assigned to the following 3 groups: Group ...

ea0014p645 | (1) | ECE2007

Effect of age and testosterone on sleep related erections

Granata Antonio R M , Rochira Vincenzo , Madeo Bruno , Pugni Valeria , Zirilli Lucia , Carani Cesare

Introduction: In order to study the effect of age and testosterone on sleep related erections, we enrolled 209 men (122: age 30–49 years; 87: age over 50 years), including mild and severe hypogonadal subjects (129) and eugonadal subjects (80).Subjects and methods: The subjects were assigned to four groups, according to their testosterone serum levels. All the subjects underwent nocturnal penile tumescence and rigidity monitoring (NPTRM). The followi...

ea0037oc4.3 | Diabetes | ECE2015

Chronic, long-term administration of Vardenafil improves endothelial function and corrects hypogonadism in patients with type 2 diabetes mellitus. A longitudinal, prospective, randomized, placebo-controlled, double blind, clinical trial

Santi Daniele , Guidi Alessandro , Granata Antonio , Pignatti Elisa , Bozic Roberto , Zaza Stefano , Roli Laura , Trenti Tommaso , Carani Cesare , Simoni Manuela

Background: Endothelial dysfunction leads to cardiovascular complications in type 2 diabetes mellitus (T2DM), through a reduction of nitric oxide (NO)-mediated relaxation. Phosphodiesterase-5 inhibitors (PDE5i) have hemodynamic effects, improving NO levels.Aim: To investigate if long term, chronic treatment with the PDE5i Vardenafil improves systemic endothelial function in men with T2DM.Methods: A longitudinal, prospective, invest...

ea0032p84 | Bone and Osteoporosis | ECE2013

Effects of treatment for acromegaly on bone mineral density: is pegvisomant protective on lumbar BMD?

Brigante Giulia , Santi Daniele , Diazzi Chiara , De Vincentis Sara , Ferrannini Giulia , Madeo Bruno , Carani Cesare , Simoni Manuela , Rochira Vincenzo

Background: GH–IGF1 status is important for bone health. Acromegaly affects bone status, but less is known on the role of treatments for acromegaly on bone mineral density (BMD). Pegvisomant (Peg) is effective in treating acromegaly by reducing IGF1. As serum GH is not influenced by Peg, it is not known if residual, direct GH effects on bone (not IGF1 mediated) are preserved during treatment.Methods: To evaluate the effects of Peg on BMD, we compare...

ea0032p640 | Male reproduction | ECE2013

Complete aromatase deficiency in four adult men: detection of a novel mutation and two known mutations in the CYP19A1 gene

Pignatti Elisa , Unluhizarci Kursad , Kartal Ermine , Ajlouni Kamel , Khawaja Nahla , Carani Cesare , Marino Marco , Simoni Manuela , Vighi Eleonora , Rochira Vincenzo

Introduction: At present, only eight men with loss-of-function mutations in the CYP19A1 gene have been described. Here we report the genetic study of four adult men with undetectable serum estrogens, unfused epiphyses, eunuchoid skeletal proportions, continuing linear growth, tall stature, genu valgum, osteoporosis, obesity and achantosis nigricans. Patient 1 (26-years/182 cm) and 2 (28-years/187 cm) are from Turkey with a history of consanguinity. Patient 3 (44-years...