Searchable abstracts of presentations at key conferences in endocrinology

ea0016s12.3 | Addicted to food? | ECE2008

Hypothalamic regulation of energy balance

Cota Daniela

Obesity and its consequences, such as type-2 diabetes, cardiovascular disease and cancer, are serious health threats. However, despite what the obesity epidemic might suggest, the balance between caloric intake and expenditure is regulated with tremendous precision under most circumstances. Thus, a regulatory system exists, where deviations from the defended body adiposity level trigger signals that can be monitored by specific intracellular metabolic pathways. Such pathways a...

ea0037gp.09.08 | Nuclear receptors and signalling | ECE2015

Study of a possible interaction between reactive oxygen species and the mTORC1 pathway in the regulation of energy balance

Haissaguerre Magalie , Clark Samantha , Tabarin Antoine , Cota Daniela

Obesity and its consequent metabolic disorders are severe health problems. The mechanistic target of rapamycin complex 1 (mTORC1) pathway is an important hypothalamic integrator of the actions of nutrients and hormones on food intake (FI). Nutrient availability also affects the formation of reactive oxygen species (ROS) in the hypothalamus and regulates neuronal activity. In the present study, we hypothesise that modulation of mTORC1 activity mediates ROS effects on FI. To thi...

ea0014oc10.1 | Obesity & metabolism | ECE2007

The selective neuronal deletion of cannabinoid type 1 receptor is still able to provide resistance to diet-induced obesity

Cervino Cristina , Cota Daniela , Bellocchio Luigi , Mancini Giacomo , Lutz Beat , Marsicano Giovanni , Pasquali Renato , Pagotto Uberto

It is well known that cannabinoid type 1 receptor (CB1) antagonist drugs may reduce body weight and improve metabolic profiles in obese animals and humans by a double mechanism: at first, targeting mesolimbic and hypothalamic nuclei and, thereafter, peripheral organs involved in energy storage and expenditure. However, it is still unknown which of these sites of action may have a predominant role in the endocannabinoid effect on energy balance regulation. To solve this questio...

ea0073oc12.6 | Oral Communications 12: Diabetes, Obesity, Metabolism and Nutrition | ECE2021

Molecular and functional mapping of POMC neuronal heterogeneity in obesity

Léon Stéphane , Simon Vincent , Clark Samantha , Dupuy Nathalie , Cota Daniela , Quarta Carmelo

The brain is critically involved in the regulation of energy balance and glucose homeostasis. Depending on the levels of energy available in our body, the activity of a group of hypothalamic neurons expressing the neuropeptidergic marker proopiomelanocortin (POMC) changes and it plays a key role in maintaining energy balance. When POMC neuronal activity is altered, this can lead to impaired energy homeostasis and therefore to obesity. However, POMC neurons are highly diverse, ...

ea0081oc10.1 | Oral Communications 10: Diabetes, Obesity, Metabolism and Nutrition 3 | ECE2022

Single-cell molecular and functional mapping of POMC neurons in obesity: a multi-modal approach

Leon Stephane , Simon Vincent , Lee Thomas H , Clark Samantha , Dupuy Nathalie , Le Feuvre Yves , Fioramonti Xavier , Cota Daniela , Quarta Carmelo

The brain plays a crucial role in maintaining the body’s energy needs, a process involving the activity of a group of hypothalamic neurons that express the neuropeptidergic marker pro-opiomelanocortin (POMC). POMC neuronal dysfunction can cause obesity and its associated metabolic sequelae. However, this population of neurons is highly diverse at a molecular and functional level, and whether or not such heterogeneity is implicated in disease establishment or progression h...

ea0063oc6.3 | Obesity | ECE2019

Role of hypothalamic bile acid-TGR5 signaling in the regulation of energy balance

Castellanos Ashley , Quevedo Omar Guzman , Fenelon Valerie , Zizzari Philippe , Clark Samantha , Dupuy Nathalie , Bellocchio Luigi , Marsicano Giovanni , Cota Daniela

Bile acids (BA) are cholesterol-derived molecules that are mostly known for their involvement in lipid digestion and absorption. Recent evidence recognizes them as metabolic integrators able to reduce body weight (BW), increase energy expenditure (EE) and improve glycemic control by activating their specific receptor, the Takeda G protein-coupled receptor 5 (TGR5) in peripheral organs. These outcomes have increased importance in the context of diet-induced obesity and associat...

ea0081oc10.3 | Oral Communications 10: Diabetes, Obesity, Metabolism and Nutrition 3 | ECE2022

Glucose-dependent insulin secretion is regulated by mitochondrial-associated cannabinoid receptor type 1 (mtCB1)

Allard Camille , Zizzari Philippe , Clark Samantha , Dupuy Nathalie , Bermudez-Silva Francisco-Javier , Delgado Concepcion Lillo , Bellocchio Luigi , Marsicano Giovanni , Quarta Carmelo , Cota Daniela

Type 2 diabetes is among the most prevalent chronic diseases worldwide. Further research is needed to identify new mechanisms that may help preserve the function of insulin-secreting pancreatic β-cells. The cannabinoid receptors type 1 (CB1) and their endogenous ligands, endocannabinoids, exert a key role in regulating of glucose homeostasis, β-cells function, and insulin secretion. Intriguingly, CB1 is not only located at the plasma membrane (pmCB1), but also at the...

ea0070ep169 | Diabetes, Obesity, Metabolism and Nutrition | ECE2020

Peripheral cannabinoid–1 receptor blockade potentiates the anti–obesity and anti–diabetic effects of GLP–1 mimetics

Zizzari Philippe , He Rongjun , Bellocchio Luigi , Allard Camille , Clark Samantha , Thierry Leste–lasserre , Marsicano Giovanni , Perez–Tilve Diego , Finan Brian , Cota Daniela , Quarta Carmelo

Obesity and type–2 diabetes (T2D) represent a global health crisis. However, only a few, and often sub–effective pharmacological tools are available to treat these disorders. Though beneficial for glycemic control, GLP–1 receptor (GLP–1R) agonists show scarce weight–lowering and insulin–sensitizing efficacy.New molecules able to selectively block the activity of the cannabinoid receptor type–1 (CB1R) in periphera...

ea0022h2.1 | Oral Communications Highlights 2 | ECE2010

ESE Young Investigator Award

Quarta Carmelo , Bellocchio Luigi , Mancini Giacomo , Mazza Roberta , Cota Daniela , Braulke Lutzie J , Fekete Csaba , Nisoli Enzo , Pasquali Renato , Lutz Beat , Marsicano Giovanni , Pagotto Uberto

The pharmacological blockade of cannabinoid receptor type 1 (CB1) has been shown to counteract diet induced obesity (DIO). An unsolved question is whether the effects of CB1 blockade on energy balance are due to peripheral or central mechanisms. This is an important clinical issue since the CB1 antagonist drugs have recently been withdrawn from the market due to their psychiatric side effects, and novel drugs selectively acting at peripheral le...