Searchable abstracts of presentations at key conferences in endocrinology

ea0022p823 | Thyroid | ECE2010

DNA repair genes in the thyroid cell lines and human tumors

Janik Justyna , Hanusek Karolina , Czarnocka Barbara

Free radicals and reactive oxygen species (ROS) participate in physiological and pathological processes in the thyroid gland. Oxidative stress caused by ROS is involved in many types of DNA damage that are associated with the initiation or the progression of numerous human cancers including thyroid tumors. One of the most mutagenic products of oxygen radical forming agents is 7,8-dihydro-8-oxoguanine (8-oxoG). The aim of the current study was to investigate expression of OGG1 ...

ea0016p41 | Adrenal | ECE2008

Addison's disease: aetiological role of oncological factors

Kasperlik-Zaluska Anna , Czarnocka Barbara , Bednarek-Papierska Lucyna , Zgliczynski Wojciech

Autoimmune destruction of the adrenocortical cells is the most frequent form of Addi-son’s disease, however modern imaging methods make possible to discover bilateral neoplastic laesions resulting in adrenal insufficiency more frequently than about twenty years ago. In our group of 315 patients with Addison’s disease we diagnosed metastatic infiltrations of the adrenals in 18 cases and lymphoma of both adrenals in four cases (in sum 7%). Typical clinical signs, bioch...

ea0035p1131 | Thyroid Cancer | ECE2014

The role of the transcription factor: Prospero homeobox 1: in the biology of differentiated thyroid cancer

Rudzinska Magdalena , Gawel Damian , Karpinska Kamila , Kierdrowski Miroslaw , Stepien Tomasz , Marchlewska Magdalena , Domek Hanna , Czarnocka Barbara

One of the pathways of DTC dissemination is the lymphatic system, however the molecular basis of this process is still unknown. One of the key regulators of lymphangiogesis is Prospero homeobox 1 (Prox1) specifically expressed in LEC and in some human cancers. The present study investigates the expression and function Prox1 in biology of differentiated cancer cells.Studies were performed in a series of thyroid cancer FTC and PTC cell lines. Protein and t...

ea0035p1135 | Thyroid Cancer | ECE2014

The role of podoplanin in the biology of differentiated thyroid cancers

Karpinska Kamila , Rudzinska Magdalena , Gawel Damian , Kiedrowski Miroslaw , Stepien Tomasz , Marchlewska Magdalena , Hanna Domek , Czarnocka Barbara

Podoplanin (PDPN), a mucine type transmembrane glycoprotein specific to lymphatic system is expressed in a variety of human tumors and regarded as a factor promoting tumor progression. In a recent work, we found PDPN expression in tumor cells of 40% of papillary thyroid carcinomas (PTC), however it role remains unclear. The purpose of this study was to elucidate the molecular role of PDPN in the biology of thyroid cancer cells.PDPN gene and prot...

ea0020oc1.6 | Endocrine Tumours | ECE2009

Implication of the Wnt/β-catenin signaling pathway activation in the adenoma and in the malignant adrenocortical cancer

Czarnocka Barbara , Kasperlik-Zaluska Anna , Bardadin Krzysztof , Roszkowska Katarzyna , Cichocki Andrzej

The commonly expressed multifunctional protein and protooncogene β-catenin displays important functions in cell–cell adhesion and in the Wnt signaling pathway. Transforming activity of the Wnt/β-catenin pathway is thought to be involved in the development of a variety of human cancers and many β-catenin target genes have been shown to play critical functions in tumors. Therefore, the aim of this project was to determine the expression levels and cellular di...

ea0016p687 | Thyroid | ECE2008

Expression of RNA-binding proteins TIAR and TIA-1 in thyroid tissues from patients with immune and non-immune thyroid diseases

Bossowski Artur , Czarnocka Barbara , Bardadin Krzysztof , Urban Miroslawa , Czerwinska Jolanta , Lyczkowska Anna , Dadan Jacek

TIAR and TIA-1 are two closely related RNA-binding proteins, which possess three RNA recognition motifs (RMMs). These proteins are involved in several mechanisms of RNA metabolism, including regulation of mRNA translation. In cellular apoptosis continuously TIAR shuttled between the cytoplasm and nucleus.The aim of this study was to estimate expression of closely related mRNA binding proteins TIAR and TIA-1 in thyroid tissues from young patients with Gra...

ea0014p553 | (1) | ECE2007

Growing incidence of idiopathic isolated secondary adrenal insufficiency.

Kasperlik-Zaluska Anna , Czarnocka Barbara , Papierska Lucyna , Bensing Sophie , Hulting Anna , Crock Patricia

Objective: The origin of idiopathic isolated secondary adrenal insufficiency (IISAI) is uncertain, however autoimmunity seems to be the most probable cause. Within last eight years the initial number of about 100 such cases, increased in our registry by 250%. We searched for features of autoimmune diseases in our group of patients to prove autoimmune etiology in a majority of these patients.Materials and methods: The material consisted of 260 patients wi...

ea0022p12 | Adrenal | ECE2010

Autoimmune disorders in Addison's disease and in isolated secondary adrenal insufficiency

Kasperlik-Zaluska Anna A , Czarnocka Barbara , Jeske Wojciech , Bednarek-Papierska Lucyna , Hulting Anna-Lena , Bensing Sophie , Crock-Ludecke Patricia , Kampe Olle

Autoimmunity is the most frequent cause of Addison’s disease (AD). Isolated secondary adrenal insufficiency (ISAI) in patients not treated with corticosteroids seems to be an autoimmune disease too. In the registry of the Department of Endocrinology in Warsaw we have 328 patients with AD and 305 patients with ISAI. Autoimmune AD was diagnosed in 262 of 328 patients (77%) and in 217 of them (83%) some associated autoimmune disorders were found, while in the group with ISAI...

ea0020p13 | Adrenal | ECE2009

Autoimmune adrenal insufficiency: Addison's disease versus idiopathic isolated secondary adrenal insufficiency

Kasperlik-Zaluska Anna , Czarnocka Barbara , Jeske Wojciech , Papierska Lucyna , Hulting Anna-Lena , Bensing Sophie , Crock Patricia , Kampe Olle

Studies on autoimmune Addison’s disease (AD) have a long tradition, while knowledge on idiopathic isolated secondary adrenal insufficiency (SAI), which also seems to be caused by autoimmunity, is not so popular. Our presentation aimed at comparing two groups of patients: 1) 238 patients with AD (183 women, F/M=4.3), aged 8–61 years at time of diagnosis and 2) 301 patients with SAI (269 women, F/M=8.4), aged 17–87 years at time of diagnosis. Maximum time of durat...

ea0037gp.24.01 | Thyroid–genetics | ECE2015

Prox1 transcription factor is differentially expressed in thyroid cancer and contributes to the regulation of invasion and migration of thyroid cancer cells

Rudzinska Magdalena , Gawel Damian , Karpinska Kamila , Kiedrowski Miroslaw , Stepien Tomasz , Marchlewska Magdalena , Krasuska Wanda , Ledwon Joanna , Macios Maria , Lange Dariusz , Czarnocka Barbara

Introduction: The differentiated thyroid cancer (DTC) is the most frequent endocrine malignancy with the incidence increasing worldwide. Although, DTCs are considered to be mostly indolent lesions, the proportion of patients develop metastases. Therefore, identification of molecular factors that are involved in thyroid tumour progression might help to better understand the mechanism of thyroid cancer metastasis. In the present study we examine the role of the transcription fac...