Searchable abstracts of presentations at key conferences in endocrinology

ea0063gp142 | Interdisciplinary Endocrinology 1 | ECE2019

Impact of age and metabolic profile on the definition of 12 circulating steroid reference intervals measured by LC-MS/MS in an Italian healthy adult male cohort

Mezzullo Marco , Di Dalmazi Guido , Fazzini Alessia , Ibarra Gasparini Daniela , Pelusi Carlotta , Pagotto Uberto , Fanelli Flaminia

Background: Steroid profiling by LC-MS/MS is increasingly used in endocrine research, and is being introduced in the clinical practice. Due to the large disagreement with previous immunoassays, and to the enlarged panel of measurable steroids, novel and robust reference intervals (RIs) need to be established. Steroid circulating levels are known to be influenced by several physio-pathological factors, such as age, gender, stress and metabolic status. Therefore, generating unbi...

ea0063gp143 | Interdisciplinary Endocrinology 1 | ECE2019

Impact of age, menstrual phase, menopausal status and metabolic profile on the definition of 12 circulating steroid reference intervals measured by LC-MS/MS in an Italian healthy adult female cohort

Mezzullo Marco , Di Dalmazi Guido , Fazzini Alessia , Ibarra Gasparini Daniela , Gambineri Alessandra , Pagotto Uberto , Fanelli Flaminia

Endocrine clinical practice strongly relies on normative data for the assessment of hormonal imbalance. However, the generation of robust steroids reference intervals (RIs) remains a challenge. As the steroids circulating levels are influenced by many procedural aspects related to blood collection (time, fasting state) and physio-pathological factors, such as age, sex, stress and metabolic status, the need for partitioning RIs is mandatory. Furthermore, unbiased healthy, drug-...

ea0037gp.05.08 | Developmental and paediatric endocrinology | ECE2015

A simple and rapid method for steroid profiling by two dimensional liquid chromatography–tandem mass spectrometry: toward routine application

Fanelli Flaminia , Mezzullo Marco , Fazzini Alessia , Pedercini Matthew , Pasquali Renato , Pagotto Uberto

Steroid testing has a central role in clinical decision-making and in research studies on diseases such as hypercortisolisms, female hyperandrogenism, male hypogonadism or inborn disorders of steroid synthesis. Recently, liquid chromatography–tandem mass spectrometry (LC–MS/MS) proved its superiority to routine immunoassays (IA) in accurately and sensitively measuring low level steroids. However, the replacement of automated IA with LC–MS/MS platforms is limited...

ea0081ep879 | Reproductive and Developmental Endocrinology | ECE2022

Daytime salivary androgen rhythm by LC-MS/MS in women affected by Polycystic Ovary Syndrome (PCOS) fulfilling the three Rotterdam diagnostic criteria

Fanelli Flaminia , Hawley James M , Mezzullo Marco , Fazzini Alessia , Zanotti Laura , Magagnoli Matteo , Pagotto Uberto , Keevil Brian , Gambineri Alessandra

Background: Excess testosterone in PCOS is accompanied by increased levels of other ovarian and adrenal androgens and precursors. Recently, 11-oxygenated adrenal androgens have been postulated as major components of the circulating androgen pool in PCOS. Obesity is strictly connected with androgen excess and with the derangement of hormone circadian rhythmicity. To date, it has not been clarified whether hyperandrogenism in PCOS, either complicated by obesity or not, is accomp...

ea0041oc10.5 | Reproduction & Endocrine Disruption | ECE2016

Effect of clomiphene citrate and metformin on testosterone levels in hypogonadal obese men with impaired glucose tolerance (IGT) or type 2 diabetes (DM2)

Pelusi Carla , Giagulli Vito , Baccini Margherita , Fanelli Flaminia , Mezzullo Marco , Fazzini Alessia , Casadio Elena , Prontera Olga , Pagotto Uberto , Pasquali Renato

Low testosterone (T) levels are often found in obese men with metabolic abnormalities, however the mechanism underling this condition is unclear. This study aimed to evaluate the effectiveness of clomiphene citrate (CC) in increasing T levels and improving metabolic control in hypogonadal obese men with IGT or DM2. A randomized cross-over double blind controlled study was conducted in twenty obese hypogonadal caucasian men, mean age 47 ys, classified in IGT (n=11) or ...

ea0041ep658 | Female Reproduction | ECE2016

Sex hormone and steroid precursor measurement by simple and rapid liquid chromatography-tandem mass spectrometry (Lc-Ms/Ms) method: comparison with current routine immunoassays

Fanelli Flaminia , Mezzullo Marco , Fazzini Alessia , Baccini Margherita , Gambineri Alessandra , Vicennati Valentina , Pelusi Carla , Pasquali Renato , Pagotto Uberto

Though LC-MS/MS is replacing immunoassays for major glucocorticoids and androgens routine assessment, the measurement of other sex steroids and precursors is still challenging and available only in experienced research laboratories. Our aim was to develop a simple-prep and rapid 2D-LC-MS/MS method for the simultaneous measurement of estrone (E1), estradiol (E2), dihydrotestosterone (DHT) and 17OHpregnenolone.Serum volumes of 300ul were spiked with intern...

ea0056p927 | Female Reproduction | ECE2018

Disentangling polycystic ovary syndrome (PCOS) components by liquid-chromatography – tandem mass spectrometry (LC-MS/MS) steroid profiling

Fanelli Flaminia , Mezzullo Marco , Zanotti Laura , Fazzini Alessia , Mastroroberto Marianna , Morselli-Labate Antonio Maria , Pagotto Uberto , Pasquali Renato , Gambineri Alessandra

Introduction: Clinical and laboratory hyperandrogenisms indistinctly contribute to PCOS diagnosis; however, they differ in hormonal and metabolic correlates and require proper therapeutic strategies. Circulating biomarkers of hirsutism were not identified so far, with elevated testosterone being observed only in half of hirsute patients. Reasons for such inconsistencies possibly relies in previously used immunoassays, nowadays recognized as not adequate because of poor reliabi...

ea0049ep1166 | Male Reproduction | ECE2017

Testosterone is poorly related to erectile dysfunction in young/middle aged human immunodeficiency virus-infected men

Vincentis Sara De , Santi Daniele , Decaroli Maria Chiara , Fanelli Flaminia , Mezzullo Marco , Fazzini Alessia , Ansaloni Anna , Pagotto Uberto , Guaraldi Giovanni , Rochira Vincenzo

Background: human immunodeficiency virus (HIV)-infection is strongly associated to erectile dysfunction (ED) in men. Preliminary data suggests that ED is poorly associated with serum T in HIV+ men.Aim: To investigate in HIV-infected men the relationship between sexual function as assessed by the validated International Index of Erectile Function (IIEF-15) and T deficiency using Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS).<p class="abste...

ea0041ep784 | Obesity | ECE2016

The circulating fingerprint revealed by targeted metabolomic as biomarker of metabolic impairment in female overweight/obesity

Mezzullo Marco , Fanelli Flaminia , Fazzini Alessia , Baccini Margherita , Casadio Elena , Ibarra-Gasparini Daniela , Mazza Roberta , Vicennati Valentina , Fontanesi Luca , Pasquali Renato , Pagotto Uberto

The comprehension of the network of metabolic processes may help the understanding of the molecular pathways driving obesity and related complications. However this is a hard task due to the large number of actors and their complex interplay. We aimed at exploring by targeted metabolomic the circulating metabolite profile in lean (NW, n=42; BMI: 18.5–24.9 kg/m2) and age-matched overweight/obese (OB, n=37, BMI≥25.0 kg/m2), drug...