Searchable abstracts of presentations at key conferences in endocrinology

ea0037ep37 | Adrenal cortex | ECE2015

Pregnancy-induced Cushing's syndrome

Andreescu Corina , Hofland Leo , Hofland Hans , Looijenga Leendert , de Herder Wouter , Feelders Richard

CushingÂ’s syndrome (CS) during pregnancy is a rare metabolic condition with only a few cases reported in the literature. Misdiagnosis of CS is common because of the overlapping features of fatigue, weight gain, striae, and emotional changes that occur during normal pregnancy. The clinical presentation together with laboratory and imaging findings help to make a diagnosis. However, changes in maternal hormones and their binding proteins complicate assessment of the normal ...

ea0013s1 | Society for Endocrinology Dale Medal Lecture | SFEBES2007

The ever expanding role of somatostatin receptors in endocrine disease

Lamberts Steven WJ , Hofland Leo J

Studies on the distribution of Somatostatin Receptor (sst)-subtypes on endocrine tumors, the development of sst-subtype specific analogues, the elucidation of phenomena like receptor desensitization and ligand-receptor internalization, and the potential consequences (and reversal) of the epigenetic silencing of sst offer new diagnostic and therapeutic modalities in endocrine diseases:1. apart from sst2 activation, Somatostatin analogues target...

ea0081rc6.2 | Rapid Communications 6: Endocrine-Related Cancer | ECE2022

Reciprocal interactions between fibroblast and pancreatic neuroendocrine tumor cells: putative impact of the tumor microenvironment

Cuny Thomas , Mondielli Gregoire , van Koetsveld Peter , de Herder Wouter , Barlier Anne , Hofland Leo

Introduction: Pancreatic neuroendocrine neoplasms (PNEN) present with a fibrotic stroma which constitutes the tumor microenvironment (TME). The role played by stromal fibroblasts over the growth of PNEN and their sensitivity to the mTOR inhibitor, RAD001, are as yet unestablished.Methods: We investigated reciprocal interactions between 1) human PNEN cell lines (BON-1/QGP-1) or primary cultures of human ileal neuroendocrine neoplasm (iNEN) or PNEN, and 2)...

ea0063p40 | Adrenal and Neuroendocrine Tumours 1 | ECE2019

Available 3D cultures methods: study on a Pancreatic Neuroendocrine Neoplasm cell line

Bresciani Giulia , Hofland Leo J , Giamas Georgios , Gagliano Teresa , Zatelli Maria Chiara

Background: Pancreatic Neuroendocrine Neoplasms (pNENs) are malignancies arising from the endocrine pancreas. Past in vitro studies have led to a better comprehension and characterization of this malignancy under several points of view. However, effective medical therapies are still not available, therefore, it is essential to continue studying this malignancy in order to identify a successful approach. Due to tumour complexity, techniques such as 3D cultures, able to...

ea0049ep182 | Endocrine tumours and neoplasia | ECE2017

Effects of ketoconazole on ACTH-producing and non ACTH-producing neuroendocrine tumor cells

Herrera-Martinez Aura D , Feelders Richard , Castano Justo , Dogan Fadime , Koetsveld Peter van , Hofland Leo

Prolonged spontaneous remission of hypercortisolemia in ectopic ACTH syndrome after long-term treatment with steroidogenesis inhibitors has been described. Direct drug effect on the adrenal glands, effects on tumoral ACTH secretion and/or POMC gene expression have been suggested. Medical treatment could be used for symptoms, but also for disease control.Materials and methods: In human BON-1 and DMS-79 cells, we have evaluated the effects of keto...

ea0049ep196 | Neuroendocrinology | ECE2017

Identification of molecular targets of Sunitinib in pancreatic neuroendocrine tumours

Bresciani Giulia , Gagliano Teresa , Hofland Leo J , Gentilin Erica , Falletta Simona , Riva Eleonora , Chiara Zatelli Maria

: Pancreatic neuroendocrine tumours (pNETs) are rare neoplasms arising from the endocrine pancreas. The fist line treatment is surgery that is often not curative in the presence of metastatic disease. Therefore, there is an increasing need for medical therapy. Sunitinib is a multi-target receptor tyrosine-kinase (RTK) inhibitor, described as having as main target VEGF receptor, with antitumor and antiangiogenic effects, approved for pNETs medical treatment. This study is aimed...

ea0049ep197 | Neuroendocrinology | ECE2017

Study of different in vitro systems for the evalutation of Sunitinib effects in pancreatic neuroendocrine tumour cells

Bresciani Giulia , Gagliano Teresa , Hofland Leo J , Gentilin Erica , Falletta Simona , Riva Eleonora , Chiara Zatelli Maria

Finding new preclinical models to study the effects of anticancer drugs is one of the aims of biomedical research. Indeed, testing different in vitro systems can lead to a better understanding of the molecular pathways regulating tumor development and growth, and help to find new therapeutic approaches. This is crucial especially in the settings of pancreatic neuroendocrine tumours (pNET), where one of the main drugs approved for medical treatment is Sunitinib, a mult...

ea0049ep933 | Pituitary - Basic | ECE2017

Acquired temozolomide resistance in ACTH-secreting pituitary tumour cells

Silva Ticiana , Van Koetsveld Peter , Dogan Fadime , Feelders Richard , Hofland Leo

Introduction: Temozolomide (TMZ) treatment has been used for aggressive pituitary tumours with positive results, but has proven ineffective in controlling tumour regrowth in cases of disease recurrence. TMZ resistance has been linked to the expression of O6-methyl-guanine-DNA-methyltransferase (MGMT) protein and mismatch repair components (MMR). In the present study, we describe the development of an in vitro model of acquired resistance to TMZ and the mechanisms invo...

ea0014p128 | (1) | ECE2007

Expression of the neuropeptide cortistatin in haematological malignancies

Dalm Virgil , Staal Frank , Hofland Leo , van der Lely Aart-Jan , van Hagen Martin

Introduction: Cortistatin (CST) is a 17 amino-acid neuropeptide involved in sleep regulation. Due to its structural resemblance to somatostatin (SS), CST binds with high affinity to the 5 known SS receptors. CST also binds to the putative MrgX2 receptor. Previously we demonstrated that various types of human immune cells and tissues as well as lymphoid cell lines express CST mRNA. We suggested that CST plays a regulatory role in immune cell function both in physiological and p...

ea0099ep702 | Pituitary and Neuroendocrinology | ECE2024

Long-term exposure of corticotroph pituitary tumor cells to glucocorticoid results in resistance to its anti-proliferative effect

Silva Ticiana , Dogan Fadime , Iyer Anand , van Koetsveld Peter , Feelders Richard , Hofland Leo

Background: Cortisol has a suppressive effect on corticotroph proliferation. In CushingÂ’s disease (CD), the relative resistance to glucocorticoid (GC) action may favor corticotroph tumor formation and autonomous ACTH secretion.Objective: To analyze the consequences of long-term GC exposure on corticotroph tumor cell proliferation and to evaluate the modification of gene expression attributable to prolonged GC treatment.Methods...