Searchable abstracts of presentations at key conferences in endocrinology

ea0070aep288 | Diabetes, Obesity, Metabolism and Nutrition | ECE2020

Association between the circadian rhythm and the inhibitory effect of glucocorticoids on browning of adipocytes

Congiusta Noemi , Gesmundo Iacopo , Negri Mariarosaria , Pagliaro Pasquale , Pivonello Rosario , Penna Claudia , Ghigo Ezio , Granata Riccarda

White adipose tissue (WAT) stores excess energy as triglycerides, while brown adipose tissue (BAT) dissipates energy through heat, acting as a defense against cold and obesity and as a positive regulator of metabolic functions. The thermogenic function of BAT is mainly regulated by the mitochondrial uncoupling protein 1 (UCP1) in brown adipocytes. Moreover, it has been shown that chronic exposure to glucocorticoids (GC) or synthetic GC receptor (GR) agonists inhibits the activ...

ea0081p614 | Diabetes, Obesity, Metabolism and Nutrition | ECE2022

Dopaminergic system and adipose tissue: in vitro effect of cabergoline on white, brown and beige adipogenesis and lipogenesis processes

Negri Mariarosaria , Pivonello Claudia , Amatrudo Feliciana , Patalano Roberta , Monto Tatiana , Angelis Cristina de , Simeoli Chiara , Simona Auriemma Renata , Colao Annamaria , Pivonello Rosario

Adipose tissue is an endocrine organ releasing adipokines and expressing specific markers and intracellular mediators, which regulate whole-body energy by balancing lipid accumulation and utilization through adipogenesis, the differentiation of preadipocytes into mature adipocytes associated with gradual lipid storage, and lipogenesis, the additional lipid accumulation in mature adipocytes. Adipogenesis and lipogenesis, strongly induced by insulin, occur in white adipocytes, s...

ea0063p1020 | Interdisciplinary Endocrinology 2 | ECE2019

Circulating miRNAs expression profile in in vitro muscle cell model exposed to cortisol excess

Patalano Roberta , Pivonello Claudia , Monto Tatiana , Simeoli Chiara , Negri Mariarosaria , Di Gennaro Gilda , Papa Fortuna , de Angelis Cristina , Cristina De Martino Maria , Pivonello Rosario

CushingÂ’s Syndrome (CS) is associated with a severe myopathy mainly affecting type 2 muscle fibres, with a higher prevalence in male subjects. The excess of glucocorticoids (GC) levels represents the most common cause of myopathy due to an alteration of protein metabolism. The non-coding post-transcriptional gene regulators muscle-specific microRNAs (miRNA) control muscle development and differentiation but when dysregulated lead to skeletal muscle (SM) disorder. The aim ...

ea0049gp184 | Pituitary | ECE2017

Hormonal and metabolic effects of long-term cabergoline withdrawal in patients with hyperprolactinemia

Auriemma Renata Simona , Blasio Chiara , Grasso Ludovica Francesca Stella , Galdiero Mariano , Negri Mariarosaria , Pivonello Claudia , Granieri Luciana , Colao Annamaria , Pivonello Rosario

Hyperprolactinemia is associated with abnormalities in glucose and lipid profile with development of insulin resistance and metabolic syndrome (MetS), which have been found to be improved by treatment with cabergoline (CAB). The current study aimed at investigating hormonal and metabolic effects of long-term CAB withdrawal in patients with prolactinomas. In 46 patients (37 F, 9 M, aged 34.5±11.5 yrs, 36 microadenomas and 10 macroadenomas) anthropometric (weight, BMI, wais...

ea0049ep34 | Adrenal cortex (to include Cushing's) | ECE2017

The treatment with dual release hydrocortisone in patients with adrenal insufficiency: correlation between change of the evening cortisol exposure time profile and change in metabolic profile, depression status and quality of life

Simeoli Chiara , Ferrigno Rosario , Pivonello Claudia , Auriemma Renata Simona , Negri Mariarosaria , Di Gennaro Gilda , Iacuaniello Davide , De Martino Maria Cristina , Colao Annamaria , Pivonello Rosario

Conventional glucocorticoids (CGCs) are unable to mimic physiological cortisol rhythm in adrenal insufficiency (AI), resulting in increased metabolic morbidity and impaired quality of life (QoL). Non-physiological cortisol pattern and elevated evening cortisol levels may be responsible for the increased risk of metabolic disorders observed in AI patients under CGCs. Once daily dual-release-hydrocortisone (DR-HC), which better reproduces physiological daily cortisol profile, si...

ea0049ep786 | Endocrine tumours and neoplasia | ECE2017

Potential role of vitamin D in restoring sensitivity to mTOR inhibitors in hepatocellular carcinoma (HCC): 1,25(OH)vitamin D (VitD) reverts everolimus (EVE) resistance in a hcc cell line

Pivonello Claudia , Provvisiero Donatella Paola , Negri Mariarosaria , Gennaro Gilda Di , de Angelis Cristina , Simeoli Chiara , Galdiero Giacomo , Martino Maria Cristina De , Colao Annamaria , Pivonello Rosario

HCC is a difficult-to-treat- cancer with poor prognosis. Despite EVOLVE-1 trial demonstrated that EVE did not improve overall survival in molecularly and clinically unselected patients with advanced sorafenib resistant HCC, in selected patients, the established antitumor effect of EVE could make this drug a potential adjuvant therapy. Unfortunately, the acquired EVE resistance due to the tumour adaptation to chronic drug use is a current challenge. VitD has been deemed as pote...

ea0049ep927 | Pituitary - Basic | ECE2017

Ketoconazole induces inhibition of cell viability and apoptosis in an ACTH-secreting tumour cell line model

Patalano Roberta , Pivonello Claudia , Solari Domenico , Vitulli Francesca , Iacuaniello Davide , Leo Monica De , Negri Mariarosaria , Provvisiero Donatella Paola , Cavallo Luigi Maria , Cappabianca Paolo , Colpo Annamaria , Pivonello Rosario

Chronic cortisol excess as a consequence of ACTH overproduction from a pituitary tumour is responsible for the development of CushingÂ’s disease (CD). The first-line treatment for CD is pituitary surgery, but medical treatment is an alternative second-line approach to control cortisol excess. Among pharmacological agents, the adrenal-blocking drug ketoconazole (KT), is able to control cortisol excess in the majority of patients with CD. During KT treatment, the adrenal blo...

ea0041oc8.2 | Thyroid - Translational | ECE2016

Selenium modulates apoptosis in thyroid follicular cells: characterization of molecular mechanisms

Cristina Nettore Immacolata , Maione Luigi , Passaro Carmela , Pivonello Claudia , Negri Mariarosaria , Desiderio Silvio , De Nisco Emma , Pivonello Rosario , Ungaro Paola , Portella Giuseppe , Colao Annamaria , Emidio Macchia Paolo

: Inflammation and several oxidative processes are modulated by Selenium (Se) trough the selenoproteins. Se deficiency has been associated with thyroid autoimmune diseases and hypothyroidism, while Se-supplementation reduces antibodies titles and ameliorates ultrasound pattern of the gland.Aim of the current study, is to study the molecular effects of Se-supplementation in thyroid follicular cells (FRTL5).FRTL5 cells have been star...

ea0041gp17 | Adrenal (1) | ECE2016

Improved evening and nocturnal cortisol exposure time profile in patients with adrenal insufficiency treated with dual release hydrocortisone: correlation with improvement in metabolic profile

Simeoli Chiara , Ferrigno Rosario , De Martino Maria Cristina , Auriemma Renata Simona , Pivonello Claudia , Negri Mariarosaria , Iacuaniello Davide , Di Gennaro Gilda , Colao Annamaria , Pivonello Rosario

Conventional glucocorticoids (GCs) are unable to mimic physiological cortisol rhythm in adrenal insufficiency (AI), with a significant impact on morbidity and mortality. Possible explanations are supra-physiological doses and impaired cortisol profile. In particular, elevated evening cortisol levels were related to glucose tolerance and insulin sensitivity alterations and visceral obesity. Once-daily-dual-release-hydrocortisone (OD-DR-HC), better reproducing the physiological ...

ea0041ep620 | Endocrine tumours and neoplasia | ECE2016

Potential role of the adrenolitic drug mitotane in the treatment of hepatocarcinoma (HCC): effect on cell proliferation in HCC cell lines

Pivonello Claudia , Provvisiero Donatella Paola , Negri Mariarosaria , Di Gennaro Gilda , De Angelis Cristina , Simeoli Chiara , Patalano Roberta , De Martino Maria Cristina , Colao Annamaria , Pivonello Rosario

HCC is one of the most common malignancies worldwide. Local approaches are generally preferred for patients whose disease is restricted to the liver. In patients with extrahepatic disease systemic therapy can be considered. Chemotherapy did not demonstrate convincing survival advantages in several trails for HCC patients. Presently, the kinase inhibitor sorafenib is the only approved systemic target therapy for the treatment of advanced HCC. Mitotane (dichlorodiphenildichloroe...