Searchable abstracts of presentations at key conferences in endocrinology

ea0063s25.3 | Late Breaking Session | ECE2019

Maternal smoking and high BMI disrupt thyroid gland development

Filis Panagiotis

Around half of expectant mothers worldwide are overweight or obese and 20% smoke. Exposure to such factors during fetal life is linked to diseases including obesity, cardiovascular disease, and behavioural disorders. Because thyroid hormones control fetal metabolism, cardiac output and brain development, altered fetal thyroid signalling can contribute to future disease. We examined the impact of maternal smoking and overweight/obese (body mass index, BMI, ≥25) on the thy...

ea0038p347 | Reproduction | SFEBES2015

Analysis of the human foetal gonadal proteome at 13–14 weeks of gestation

Matthews Rebecca , Filis Panagiotis , O'Shaughnessy Peter , Fowler Paul

Introduction: The human masculinisation programming window (8–14 weeks of gestation) sees testis-derived androgen drive the foetus towards a male phenotype. However, there are few systematic studies of human foetal gonad development.Aim: To conduct a pilot analysis of the foetal gonadal proteome at the end of the masculinisation programming window (13–14 weeks of gestation).Methods: Twenty-eight electively terminated foet...

ea0044oc6.5 | Pregnancy and Reproductive Health | SFEBES2016

Human placental and fetal liver molecular transporters are affected by maternal smoking

Walker Natasha , Filis Panagiotis , Soffientini Ugo , Bellingham Michelle , O'Shaughnessy Peter , Fowler Paul

Introduction: The placenta interchanges nutrients, oxygen and waste between mother and fetus, acts as a gate-keeper to protect the fetus and creates an optimal endocrine environment to maintain the pregnancy. Placental insufficiency underpins common pregnancy complications (e.g. intrauterine growth restriction, preterm birth). Perturbed expression of molecular transporter proteins in the placental syncytiotrophoblast will affect fetal exposure to harmful drugs/xenobiotics such...

ea0044p31 | Adrenal and Steroids | SFEBES2016

Maternal smoking disrupts adrenal steroid production in the human fetus

Johnston Zoe , Bellingham Michelle , Hough Denise , Filis Panagiotis , O'Shaughnessy Peter , Fowler Paul

Introduction: The adrenal gland dominates in human fetal steroid endocrinology and produces large amounts of Δ5 androgens. Adrenal development in the human is poorly understood, and species differences make animal models only partially relevant. In this study we measured the steroid content of the human adrenal during fetal development and determined whether maternal smoking affects adrenal steroid concentrations or associated steroidogenic enzymes....

ea0044p34 | Adrenal and Steroids | SFEBES2016

The human fetal adrenal proteome: development, sex, and maternal smoking link in utero smoke-exposure to offspring disease

Filis Panagiotis , Johnston Zoe , Bellingham Michelle , Sofientini Ugo , O' Shaughnessy Peter , Fowler Paul

Introduction: The human fetal adrenal has unique structure/function and produces hormones (DHEA, corticoids, catecholamines) that control fetal development, organ maturation and parturition. Maternal smoking during pregnancy has immediate (pre-term delivery, low birth weight) and long-term effects on the offspring (metabolic syndrome, disrupted adrenal function). We performed shotgun proteomics to characterise human fetal adrenal development and to explore adverse effects of m...

ea0038p142 | Growth and development | SFEBES2015

Human foetal adrenal gland development and effects of maternal smoking

Johnston Zoe C , Filis Panagiotis , Bellingham Michelle , O'Shaughnessy Peter J , Fowler Paul A

Introduction: The human foetal adrenal gland (HFA) is a highly active endocrine organ, producing large amounts of DHEA and DHEAS. Our understanding of HFA development is limited, however, and species differences mean that animal models are only of partial use. Maternal cigarette smoking is known to increase the post-natal risk of health complications of the foetus, and the mechanisms involved may include effects on the HFA.Aim: To examine normal HFA deve...

ea0070aep810 | Reproductive and Developmental Endocrinology | ECE2020

Prenatal programming of hepatic lipid metabolism: Sex, hormones and lifelong health

Siemienowicz Katarzyna , Filis Panagiotis , Talia Chiara , Thomas Jennifer , Fowler Paul , Duncan Colin , Rae Mick

Background: The potential for a healthy life is programmed by in utero development. Fetal development is impacted by perturbed hormonal signalling, with lifelong consequences. Polycystic Ovary Syndrome (PCOS), affecting over 10% of women, is an important condition linked to an altered prenatal endocrine environment. Women with PCOS have increased androgen concentrations, including during pregnancy. Increased prenatal androgen exposure is associated with a PCOS-phenoty...

ea0063p669 | Interdisciplinary Endocrinology 1 | ECE2019

Dyslipidaemia and altered hepatic function in males - consequences of androgen excess in fetal life

Siemienowicz Katarzyna , Filis Panagiotis , Shaw Sophie , Douglas Alex , Thomas Jennifer , Howie Forbes , Fowler Paul , Duncan Colin , Rae Mick

Introduction: Adult male offspring of women with PCOS have increased dyslipidaemia, characterised by elevated triglycerides (TG), increased total and LDL-cholesterol (LDL-C), and hyperinsulinaemia. As altered intrauterine endocrine environments can ‘programme’ adverse health outcomes in adulthood we hypothesised that this dyslipidaemia was a consequence of a hyperandrogenic intrauterine environment. We used an outbred large animal model to identify if prenatal androg...

ea0063p1009 | Interdisciplinary Endocrinology 2 | ECE2019

Decreased hepatic detoxification potential in males - consequences of androgen excess in fetal life

Siemienowicz Katarzyna , Filis Panagiotis , Shaw Sophie , Douglas Alex , Thomas Jennifer , Howie Forbes , Fowler Paul , Duncan Colin , Rae Mick

Introduction: Altered intrauterine endocrine environments can ‘programme’ adverse health outcomes. Linkage between altered androgen exposure in utero and adverse offspring health is robust. For example, increased maternal androgen concentrations and PCOS in female offspring and dyslipidaemia in male offspring. We hypothesised that the liver was a major target for androgenic programming in utero and hepatic dysfunction would be present in offspring. ...

ea0038p250 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

Lifelong exposure to sewage sludge chemicals causes proteome-wide and sex-specific disturbances in the liver

Filis Panagiotis , Eaton-Turner Emily , Bellingham Michelle , Amezaga Maria , Mandon-Pepin Beatrice , Evans Neil , Sharpe Richard , Cotinot Corinne , Rhind Stewart , O'Shaughnessy Peter , Fowler Paul

Introduction: A complex cocktail of endocrine-disrupting and other chemicals is ubiquitous in the modern environment. Exposure to such chemicals contributes to diseases including metabolic syndrome and infertility. The liver is the primary defence organ against xenotoxicants, but also the source of major of plasma proteins, and growth factors/hormones.Aim: To understand how chronic exposure to complex mixtures of chemicals at human and environmentally re...