Searchable abstracts of presentations at key conferences in endocrinology

ea0070s4.1 | Unveiling signatures in pituitary neuroendocrine tumours | ECE2020

Circulating microRNAs: from PitNET pathogenesis to diagnostics

Butz Henriett

Introduction: MicroRNAs are short, single-stranded, protein non-coding RNA molecules which can be secreted into the circulation by mammalian cells. Their altered expression pattern has been described in many different physiological and pathological conditions. In the extracellular compartments they are encapsulated within vesicles, associated to proteins or apoptotic bodies. Due to their stability they are suggested as promising circulating biomarkers. Regarding pituitary aden...

ea0037ep162 | Reproduction, endocrine disruptors and signalling | ECE2015

Modulation of the circadian clock by glucocorticoid receptor in H295R cell line

Nagy Zsolt , Butz Henriett , Liko Istvan , Racz Karoly , Patocs Attila

Background: Peripheral clocks are set by different nervous, hormonal and metabolic stimuli and regulate the circadian expression of several genes. It has been demonstrated that circadian oscillation of gene expression can be detected in various cell lines in vitro.Aim: To explore whether a peripheral clock could be induced in human adrenocortical cell line H295R and what are the effects of glucocorticoids on this clock system.<p class="abste...

ea0063p711 | Pituitary and Neuroendocrinology 2 | ECE2019

Whole genome demethylation status of somatic DNA extracted from different pituitary adenoma types

Szabo Borbala , Nemeth Kinga , Meszaros Katalin , Szucs Nikolett , Czirjak Sandor , Reiniger Lilla , Patocs Attila , Butz Henriett

Background: Although the role of hypermethylation of certain tumor suppressor genes are known in pituitary adenomas little information is available regarding whole methylation-demethylation status and especially regarding its correlation with clinical parameters. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is an easy and accurate method to detect the level of methylcytosine (5 mC) and the demethylation intermedier hydroxymethylcytosine (5 hmC) ...

ea0025oc3.3 | Pituitary and thyroid | SFEBES2011

miR-107 inhibits the expression of aryl hydrocarbon receptor interacting protein (AIP) and is potentially involved in pituitary tumorigenesis

Trivellin Giampaolo , Igreja Susana , Garcia Edwin , Chahal Harvinder , Butz Henriett , Patocs Attila , Grossman Ashley , Korbonits Marta

Background: Abnormal microRNAs (miRNAs) expression profiles have been recently associated with sporadic pituitary adenomas, suggesting that miRNAs can contribute to tumor formation. miRNAs are small noncoding RNAs which inhibit post-transcriptional expression of target mRNAs by binding to complementary sequences usually located in the 3′ untranslated region (3′UTR). However, the substantial lack of knowledge about miRNAs’ targets hinder full understanding of t...

ea0020p566 | Neuroendocrinology, Pituitary and Behaviour | ECE2009

MicroRNA expression in human sporadic pituitary adenomas

Butz Henriett , Liko Istvan , Boyle Belema , Czirjak Sandor , Igaz Peter , Patocs Attila , Racz Karoly

Introduction: MicroRNAs (miRs) are 16–29 nucleotide long, non-coding RNA molecules that post-transcriptionally regulate gene expression via RNA interference. It has been shown that they participate in control of cell proliferation, cell differentiation, signal transduction, cell death and carcinogenesis.Aim: To examine the role of the miRs in sporadic pituitary tumourigenesis.Methods: Twenty-five sporadic pituitary adenoma spe...

ea0037gp.18.06 | Pituitary–Basic and IGF-1 | ECE2015

Comparison of high-throughput platforms in evaluation of whole genome miRNA expression profiles in pituitary tissues

Butz Henriett , Darvasi Otto , Szabo Peter M , Liko Istvan , Pongor Lorinc , Czirjak Sandor , Racz Karoly , Patocs Attila

Introduction: There are three principal high-throughput methods that have been widely used to determine whole genome miRNAs expression profiles: i) microarrays, ii) qPCR based arrays, and iii) next generation sequencing (NGS). Our aim was to compare the results obtained from these platforms in normal and adenomatous pituitary samples and to validate the results by an independent sample set using qPCR.Material and methods: Using four normal pituitary (NP)...

ea0056p125 | Endocrine tumours and neoplasia | ECE2018

Heterogeneous genetic background of Hungarian patients with pheochromocytoma/paraganglioma requires gene panel testing

Sarkadi Balazs , Zakarias Sara , Liko Istvan , Grolmusz Vince Kornel , Butz Henriett , Toth Miklos , Szucs Nikolette , Igaz Peter , Patocs Attila

Introduction: Pheochromocytomas and paragangliomas (Pheo/PGL) are rare neuroendocrine tumours arising from the adrenal medulla or the symphathetic paraganglia, respectively. Germline mutations are present in ~40% of the patients. To date, at least 16 genes have been demonstrated to be involved in the genetic background of Pheo/PGL. Prioritization in order of genes tested can be applied, but if the probability of a disease-associated germline mutation exceeds 10% the testing of...

ea0056p779 | Pituitary - Basic | ECE2018

Next generation sequencing for characterization of mitochondrial genome in pituitary adenomas

Nemeth Kinga , Darvasi Otto , Liko Istvan , Szucs Nikolette , Czirjak Sandor , Reiniger Lilla , Szabo Borbala , Igaz Peter , Patocs Attila , Butz Henriett

Introduction: Disrupted mitochondrial functions and genetic variations of mitochondrial DNA (mtDNA) have been observed in different tumors. Regarding pituitary adenomas mtDNA was evaluated only in oncocytic type using PCR based methods and it showed high prevalence of Complex I variants. Next generation sequencing (NGS) allows high throughput sequencing and it is useful for accurate identification of heteroplasmy of mitochondrial genome as well.Aim: We a...

ea0073aep3 | Adrenal and Cardiovascular Endocrinology | ECE2021

Effective metyrapon treatment of a case of neonatal cushing syndrome of unknown origin

Gacs Zsofia , Borbála Tobisch , Ágnes Sallai , Viktória Kemény , Butz Henriett , Varga Edit

Neonatal Cushing syndrome (CS) is a rather rare disease. The majority of these few cases are of ACTH dependent origin or caused by a unilateral adrenal tumour (carcinoma or adenoma), however ACTH independent bilateral hyperplasias stand for only a few percent of all cases. The management of neonatal CS depends on the underlying cause – if found in time - of the disease. In the past the survival rate of children with CS was low, new and renewed medical attempts have improv...

ea0081ep691 | Pituitary and Neuroendocrinology | ECE2022

Genomic and epigenomic aspects of Aspirin antitumoral effect in pituitary adenoma

Szabo Borbala , Krokker Lilla , Meszaros Katalin , Saskői Eva , Nemeth Krisztina , Szabo Pal , Szucs Nikolette , Czirjak Sandor , Patocs Attila , Butz Henriett

Background: In our previous works the interdependence of DNA demethylation with proliferation and differentiation of pituitary neuroendocrine tumours (PitNET) and the inhibitory effect of Aspirin on pituitary cell proliferation were demonstrated. Although the role of Aspirin in epigenetic regulation was described in other malignancies, its correlation with pituitary tumorigenesis is currently unknown.Our objective: was to investigate the genomic and epig...