Searchable abstracts of presentations at key conferences in endocrinology

ea0020oc5.2 | Thyroid: Basic and Clinical | ECE2009

Selenium and iodine determination from single murine thyroid lobes by TXRF-spectroscopy

Renko Kostja , Behrends Thomas , Stosnach Hagen , Kohrle Josef , Schomburg Lutz

Iodine (I) and selenium (Se) are two essential trace elements for regular thyroid gland functioning and thyroid hormone metabolism. Most Europeans are only marginally supplied with both elements through their daily nutrition. The mammalian organism has developed efficient and highly complex mechanisms to control I and Se uptake, metabolism and recycling. The thyroid gland is equipped with the necessary transporters and metabolizing enzymes for I uptake and bioconversion into t...

ea0020oc6.6 | Paediatric Endocrinology/Bone | ECE2009

Reduced selenoprotein P expression affects bone formation

Hog Antonia , Renko Kostja , Seemann Petra , Kornak Uwe , Schomburg Lutz

Selenium (Se) is an essential trace element and is important for maintaining health and preventing disease. Se exhibits its biological function as the 21st proteinogenic amino acid selenocysteine (Sec). Different cis and trans acting factors are necessary for incorporation of Sec into proteins. Se can be transported and stored as Sec in selenoprotein P (SePP), a liver-derived protein which contains up to 10 Sec residues per protein in humans. Thus, SePP functions...

ea0020p685 | Signal Transduction | ECE2009

On the importance of the selenium status for the inflammatory response

Stoedter Mette , Renko Kostja , Behrends Thomas , Schomburg Lutz

Many endocrine disorders bear an immunological component and involve local cytokines as paracrine signals. Moreover, certain auto-antibodies appear as causative pathological agents in some of the most common endocrine diseases e.g. in type I diabetes mellitus or different forms of autoimmune thyroid disease (AITD). Recently, selenium (Se) status and selenoproteins have emerged as important modifiers of the inflammatory response, AITD or sepsis. Notably, mortality risk of patie...

ea0016p221 | Diabetes and cardiovascular diseases | ECE2008

SelS/Tanis controls insulin sensitivity and is regulated by acute phase and diet

Stoedter Mette , Renko Kostja , Kohrle Josef , Schomburg Lutz

Background: Selenoproteins contain the 21st proteinogenic amino acid selenocysteine (Sec) and exert important biological functions. Selenoprotein S (SelS) has only recently been identified and appears to be implicated in type 2 diabetes and inflammation. In vitro studies have shown that SelS expression is controlled by circulating cytokine and glucose levels. Mechanistically, SelS participates in the retro-translocation during quality control in the ER and the ER-associ...

ea0016p353 | Growth and development | ECE2008

Impaired Se metabolism provokes gender-specific growth defects in mice

Renko Kostja , Chadt Alexandra , Michaelis Marten , Schweizer Ulrich , Al-Hasani Hadi , Kohrle Josef , Schomburg Lutz

Background: Selenoproteins are involved in oxidative stress defence, cell signalling and hormone metabolism. Accordingly, impairment of selenium (Se) metabolism or transport results in a complex phenotype as exemplified in selenoprotein P knockout mice (SePP-KO). This mouse model is characterized by a disrupted metabolism of Se resulting in neurological and growth defects. Hypothesis: Se impairs regular tissue development by modifying growth signal biosynthesis, anabolic respo...

ea0014p183 | (1) | ECE2007

Selenium supply modulates growth spurt of selenoprotein P knockout mice

Renko Kostja , Michaelis Marten , Schweizer Ulrich , Köhrle Josef , Schomburg Lutz

Background: Selenoproteins are playing an important role in body homeostasis and development. They control thyroid hormone metabolism and are of prime importance for intracellular redox regulation and cellular defence. The SePP knockout mouse (SePP-KO) is a model of impaired Se metabolism characterized by a disrupted distribution system for organified Se. One of the major phenotypes of the homozygote SePP-KO mice is a reduced increase in size and weight during the growth spurt...

ea0073oc5.1 | Oral Communications 5: Thyroid | ECE2021

Identification of iodothyronine deiodinase 2 inhibitors among FDA-approved drug library using high throughput screening

Frädrich Caroline , Renko Kostja , Wiese Niklas , Fischbach Anja , Seyffarth Carola , Neuenschwander Martin , von Kries Jens Peter , Köhrle Josef

BackgroundDevelopment and application of novel drugs frequently fail due to adverse changes of TH-levels or thyroid histology in animal experiments. Such effects potentially arise from interference with thyroid gland function or key regulators of local thyroid hormone (TH) transport, metabolism and action. Deiodinases are key enzymes in TH in-/activation, with impact on development, cell differentiation and energy metabolism among other physiological pro...

ea0073pep10.1 | Presented ePosters 10: Thyroid | ECE2021

A semi-automatic, non-radioactive 384-well high throughput screening DIO2 assay

Frädrich Caroline , Renko Kostja , Wiese Niklas , Fischbach Anja , Seyffarth Carola , Neuenschwander Martin , von Kries Jens Peter , Köhrle Josef

Thyroid hormone (TH) homeostasis depends on the coordination of several key events to maintain proper local TH signaling, including iodide uptake, hormone synthesis, metabolism, and elimination. The three isoenzymes, Deiodinases (DIO1-3), are essential components of hormone metabolism. They catalyze iodide release the outer and/or inner ring of TH structure to convert between their active and inactive forms. The activity of DIOs has been identified as an important endpoint reg...