Searchable abstracts of presentations at key conferences in endocrinology

ea0032p695 | Neuroendocrinology | ECE2013

Des-acyl ghrelin acts directly and independently and counteracts acyl-ghrelin-induced neuronal activity in the central melanocortin system of rats

Stevanovic Darko , Delhanty Patric , Themmen Axel , Popovic Vera , Holstege Joan , van der Lely Aart-Jan

Ghrelin, the endogenous GH secretagogue, has an important role in metabolic homeostasis. It exists in two major molecular forms: acylated (AG) and des-acylated (DAG). Many studies suggest different roles for these two forms in energy balance regulation. In the present study, we compared the effects of acute intracerebroventricular (ICV) administration of AG, DAG and their combination (AG+DAG) to young adult Wistar rats on food intake and central melanocortin system modulation....

ea0032p835 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

FSH and LH cells in normal-fed male rats after centrally applied ghrelin

Milosevic Verica , Ajdzanovic Vladimir , Nesic Dejan , Starcevic Vesna , Ristic Natasa , Rakocevic Rastko , Stevanovic Darko

Ghrelin, the endogenous ligand of GH secretagogue receptor type 1a, has emerged as pleiotropic modulator of diverse biological functions, including energy homeostasis and recently, reproduction. The effects of i.c.v. administered ghrelin on morphological characteristics of pituitary FSH- and LH-cells were examined in adult male Wistar rats. The animals were randomized in two groups: control and experimental group, each consisted of seven animals, and implanted with an i.c.v. c...

ea0026p190 | Neuroendocrinology | ECE2011

The role of centrally applied ghrelin on hypothalamic AMPK activity and pituitary–adrenal responses in differently fed rats

Stevanovic Darko , Milosevic Verica , Nesic Dejan , Micic Dragan , Sumarac-Dumanovic Mirjana , Popovic Vera , Starcevic Vesna

The pandemic increase of obesity, malnutrition and associated comorbidities encouraged intensive studies on energy balance control. Ghrelin is a brain-gut orexigenic hormone that is involved in both the short-term and long-term regulation of energy homeostasis. The aim of this research was to investigate the role of centrally applied ghrelin on the pituitary morphofunctional features and corticosterone secretion in normal-fed, food-restricted and obese 8 weeks old Wistar male ...

ea0022p700 | Obesity | ECE2010

Obesity and malnutrition are associated with different inflammation patterns in rats

Stevanovic Darko , Micic Dragan , Sumarac-Dumanovic Mirjana , Nesic Dejan , Popovic Vera , Starcevic Vesna , Trajkovic Vladimir

The inflammatory response in rat models of obesity and malnutrition was examined. Rats received standard chow (control), chow enriched with 30% lard (obese) or 70, 50, 40 and 40% of expected food intake weekly for 4 weeks (starved). The serum levels of cytokines were determined by ELISA, while the expression of cytokine mRNA in heart and liver was analyzed by real-time RT-PCR. The blood concentrations of pro-inflammatory cytokines (TNF, IL-1) and Th1 cytokine IFN-gamma were si...

ea0020oc2.3 | Diabetes &amp; Obesity | ECE2009

Central ghrelin administration reduces starvation-induced inflammation in rats

Stevanovic Darko , Trajkovic Vladimir , Nesic Dejan , Micic Dragan , Sumarac-Dumanovic Mirjana , Popovic Vera , Starcevic Vesna

The aim of the study was to investigate the influence of intracerebroventricular (ICV) injections of ghrelin on secretion of pro-inflammatory (TNF, IL-1, IFN-γ) and anti-inflammatory cytokines (TGF-β) in starved rats. Male Wistar rats (4 weeks old, 12 per group) were fed ad libitum or starved by caloric restriction (40% of chow consumed by their ad libitum fed controls from previous day) for 4 weeks. Afterwards, half of the animals in each group receive...

ea0020p36 | Adrenal | ECE2009

Central ghrelin modulates morphology and function of adrenal cortex in male rats

Milosevic Verica , Sosic-Jurjevic Branka , Stevanovic Darko , Terzic Milica , Nesic Dejan , Starcevic Vesna , Sekulic Milka

Ghrelin is a growth hormone secretagogue that plays an important role in appetite and weight regulation. We have previously demonstrated that central ghrelin stimulated pituitary ACTH synthesis and secretion. In this study we further examined the effects of intracerebroventricular (ICV) ad-mi-nistration of ghrelin on zona glomerulosa (ZG), zona fasciculata (ZF) and zona reticularis (ZR) of the adrenal cortex, as well as the blood concentrations of aldosterone, corticosterone a...

ea0016p568 | Obesity | ECE2008

Relationship between obesity, IL-17 and IL-23 and insulin resistance

Sumarac-Dumanovic Mirjana , Micic Dragan , Simic Maja , Stamenkovic-Pejkovic Danica , Stevanovic Darko , Jorga Jagoda , Cvijovic Goran , Zoric Svetlana , Trajkovic Vladimir

In most obese patients, obesity is associated with a low-grade inflammation of white adipose tissue resulting from chronic activation of the innate immune system and which can subsequently lead to insulin resistance, impaired glucose tolerance and even diabetes. It is still not clear role of acquired immune response in obesity and related comorbidites such as asthma, cancer or autoimmune diseases. The aim of our study was to compare level s of IL-17 and IL23 in sever obese wom...

ea0016p561 | Obesity | ECE2008

The interrelationship between leptin, visfatin and CRP levels and insulin sensitivity assessed by euglycaemic clamp in obese women

Micic Dragan , Sumarac-Dumanovic Mirjana , Stamenkovic-Pejkovic Danica , Simic Maja , Cvijovic Goran , Zoric Svetlana , Kendereski Aleksandra , Stevanovic Darko , Jorga Jagoda , Trajkovic Vladimir

Visfatin is a newly discovered adipokine found in abundance in visceral fat. Leptin is well known marker of fat mass in the body but also one aspect of leptin is its action as a proinflammatory cytokine. Recent studies, both in vitro and in vivo, indicated that visfatin is also one of the inflammatory cytokines, although the relationship between visfatin and insulin resistance remains still unclear. The aim of our study was to assess the association between visfa...