Searchable abstracts of presentations at key conferences in endocrinology

ea0032p731 | Obesity | ECE2013

Brite adipocytes: a role for cannabinoid receptor type 1 in brown adipose cell recruitment

Mazza Roberta , Quarta Carmelo , Pasquali Renato , Pagotto Uberto

Recent data showed the existence of a specific adipocyte population composed of ‘brite’ cells in white adipose tissue (WAT), characterized by a peculiar gene expression pattern including PRDM16. Despite showing a low basal UCP1 expression, these cells display higher UCP1 expression when stimulated. In view of the involvement of the endocannabinoid system in energy homeostasis, the aim of our work is to establish whether the CB1 receptor could be a target for brown ph...

ea0073oc12.6 | Oral Communications 12: Diabetes, Obesity, Metabolism and Nutrition | ECE2021

Molecular and functional mapping of POMC neuronal heterogeneity in obesity

Léon Stéphane , Simon Vincent , Clark Samantha , Dupuy Nathalie , Cota Daniela , Quarta Carmelo

The brain is critically involved in the regulation of energy balance and glucose homeostasis. Depending on the levels of energy available in our body, the activity of a group of hypothalamic neurons expressing the neuropeptidergic marker proopiomelanocortin (POMC) changes and it plays a key role in maintaining energy balance. When POMC neuronal activity is altered, this can lead to impaired energy homeostasis and therefore to obesity. However, POMC neurons are highly diverse, ...

ea0081oc10.1 | Oral Communications 10: Diabetes, Obesity, Metabolism and Nutrition 3 | ECE2022

Single-cell molecular and functional mapping of POMC neurons in obesity: a multi-modal approach

Leon Stephane , Simon Vincent , Lee Thomas H , Clark Samantha , Dupuy Nathalie , Le Feuvre Yves , Fioramonti Xavier , Cota Daniela , Quarta Carmelo

The brain plays a crucial role in maintaining the body’s energy needs, a process involving the activity of a group of hypothalamic neurons that express the neuropeptidergic marker pro-opiomelanocortin (POMC). POMC neuronal dysfunction can cause obesity and its associated metabolic sequelae. However, this population of neurons is highly diverse at a molecular and functional level, and whether or not such heterogeneity is implicated in disease establishment or progression h...

ea0041oc12.4 | Obesity | ECE2016

GLP-1 directed delivery of dexamethasone ameliorates hypothalamic inflammation and reverses diet-induced obesity

Quarta Carmelo , Clemmensen Christoffer , Yang Bin , Zhu Zhimeng , Muller Timo D , DiMarchi Richard D , Finan Brian , Tschop Matthias H

Abstract: Inflammation plays a contributing role in the pathogenesis of several metabolic disorders, including type 2 diabetes and obesity. Intriguingly, obesogenic diets induce markers of hypothalamic inflammation and neuronal injury in both rodents and humans, suggesting that targeting inflammatory pathways in the hypothalamus might represent a novel strategy to control energy metabolism. Unfortunately, the majority of steroid-based anti-inflammatory drugs are fraught with a...

ea0081oc10.3 | Oral Communications 10: Diabetes, Obesity, Metabolism and Nutrition 3 | ECE2022

Glucose-dependent insulin secretion is regulated by mitochondrial-associated cannabinoid receptor type 1 (mtCB1)

Allard Camille , Zizzari Philippe , Clark Samantha , Dupuy Nathalie , Bermudez-Silva Francisco-Javier , Delgado Concepcion Lillo , Bellocchio Luigi , Marsicano Giovanni , Quarta Carmelo , Cota Daniela

Type 2 diabetes is among the most prevalent chronic diseases worldwide. Further research is needed to identify new mechanisms that may help preserve the function of insulin-secreting pancreatic β-cells. The cannabinoid receptors type 1 (CB1) and their endogenous ligands, endocannabinoids, exert a key role in regulating of glucose homeostasis, β-cells function, and insulin secretion. Intriguingly, CB1 is not only located at the plasma membrane (pmCB1), but also at the...

ea0070ep169 | Diabetes, Obesity, Metabolism and Nutrition | ECE2020

Peripheral cannabinoid–1 receptor blockade potentiates the anti–obesity and anti–diabetic effects of GLP–1 mimetics

Zizzari Philippe , He Rongjun , Bellocchio Luigi , Allard Camille , Clark Samantha , Thierry Leste–lasserre , Marsicano Giovanni , Perez–Tilve Diego , Finan Brian , Cota Daniela , Quarta Carmelo

Obesity and type–2 diabetes (T2D) represent a global health crisis. However, only a few, and often sub–effective pharmacological tools are available to treat these disorders. Though beneficial for glycemic control, GLP–1 receptor (GLP–1R) agonists show scarce weight–lowering and insulin–sensitizing efficacy.New molecules able to selectively block the activity of the cannabinoid receptor type–1 (CB1R) in periphera...

ea0022h2.1 | Oral Communications Highlights 2 | ECE2010

ESE Young Investigator Award

Quarta Carmelo , Bellocchio Luigi , Mancini Giacomo , Mazza Roberta , Cota Daniela , Braulke Lutzie J , Fekete Csaba , Nisoli Enzo , Pasquali Renato , Lutz Beat , Marsicano Giovanni , Pagotto Uberto

The pharmacological blockade of cannabinoid receptor type 1 (CB1) has been shown to counteract diet induced obesity (DIO). An unsolved question is whether the effects of CB1 blockade on energy balance are due to peripheral or central mechanisms. This is an important clinical issue since the CB1 antagonist drugs have recently been withdrawn from the market due to their psychiatric side effects, and novel drugs selectively acting at peripheral le...

ea0016s14.6 | Basic highlights | ECE2008

Specific neuronal deletion of cannabinoid type 1 receptor (CB1) mice exhibits reduced fat mass and diet-induced obesity resistance

Cervino Cristina , Bellocchio Luigi , Quarta Carmelo , Mariani Corea Vania A , Mancini Giacomo , Vicennati Valentina , Lutz Beat , Marsicano Giovanni , Pasquali Renato , Pagotto Uberto

Aims: Recently, the endocannabinoid system emerged as a pivotal regulator of food intake, ingestive behavior and energy metabolism, acting through CB1 and its endogenous ligands, the endocannabinoids. CB1 antagonists may reduce body weight and improve metabolic profiles in animals and humans by a double mechanism: at first, they target mesolimbic and hypothalamic nuclei and, thereafter, peripheral organs involved in energy storage and expenditure. Nevertheless, it is still unk...